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Gene-targeted embryonic stem cells: real-time PCR assay for estimation of the number of neomycin selection cassettes

In the preparation of transgenic murine ES cells it is important to verify the construct has a single insertion, because an ectopic neomycin phosphortransferase positive selection cassette (NEO) may cause a position effect. During a recent work, where a knockin SCA28 mouse was prepared, we developed...

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Detalles Bibliográficos
Autores principales:	Mancini, Cecilia, Messana, Erika, Turco, Emilia, Brussino, Alessandro, Brusco, Alfredo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Short Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3226651/ https://www.ncbi.nlm.nih.gov/pubmed/22035318 http://dx.doi.org/10.1186/1480-9222-13-10

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