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Procedures for Rat in situ Skeletal Muscle Contractile Properties
There are many circumstances where it is desirable to obtain the contractile response of skeletal muscle under physiological circumstances: normal circulation, intact whole muscle, at body temperature. This includes the study of contractile responses like posttetanic potentiation, staircase and fati...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3227207/ https://www.ncbi.nlm.nih.gov/pubmed/22025076 http://dx.doi.org/10.3791/3167 |
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author | MacIntosh, Brian R. Esau, Shane P. Holash, R. John Fletcher, Jared R. |
author_facet | MacIntosh, Brian R. Esau, Shane P. Holash, R. John Fletcher, Jared R. |
author_sort | MacIntosh, Brian R. |
collection | PubMed |
description | There are many circumstances where it is desirable to obtain the contractile response of skeletal muscle under physiological circumstances: normal circulation, intact whole muscle, at body temperature. This includes the study of contractile responses like posttetanic potentiation, staircase and fatigue. Furthermore, the consequences of disease, disuse, injury, training and drug treatment can be of interest. This video demonstrates appropriate procedures to set up and use this valuable muscle preparation. To set up this preparation, the animal must be anesthetized, and the medial gastrocnemius muscle is surgically isolated, with the origin intact. Care must be taken to maintain the blood and nerve supplies. A long section of the sciatic nerve is cleared of connective tissue, and severed proximally. All branches of the distal stump that do not innervate the medial gastrocnemius muscle are severed. The distal nerve stump is inserted into a cuff lined with stainless steel stimulating wires. The calcaneus is severed, leaving a small piece of bone still attached to the Achilles tendon. Sonometric crystals and/or electrodes for electromyography can be inserted. Immobilization by metal probes in the femur and tibia prevents movement of the muscle origin. The Achilles tendon is attached to the force transducer and the loosened skin is pulled up at the sides to form a container that is filled with warmed paraffin oil. The oil distributes heat evenly and minimizes evaporative heat loss. A heat lamp is directed on the muscle, and the muscle and rat are allowed to warm up to 37°C. While it is warming, maximal voltage and optimal length can be determined. These are important initial conditions for any experiment on intact whole muscle. The experiment may include determination of standard contractile properties, like the force-frequency relationship, force-length relationship, and force-velocity relationship. With care in surgical isolation, immobilization of the origin of the muscle and alignment of the muscle-tendon unit with the force transducer, and proper data analysis, high quality measurements can be obtained with this muscle preparation. |
format | Online Article Text |
id | pubmed-3227207 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | MyJove Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-32272072011-12-02 Procedures for Rat in situ Skeletal Muscle Contractile Properties MacIntosh, Brian R. Esau, Shane P. Holash, R. John Fletcher, Jared R. J Vis Exp Physiology There are many circumstances where it is desirable to obtain the contractile response of skeletal muscle under physiological circumstances: normal circulation, intact whole muscle, at body temperature. This includes the study of contractile responses like posttetanic potentiation, staircase and fatigue. Furthermore, the consequences of disease, disuse, injury, training and drug treatment can be of interest. This video demonstrates appropriate procedures to set up and use this valuable muscle preparation. To set up this preparation, the animal must be anesthetized, and the medial gastrocnemius muscle is surgically isolated, with the origin intact. Care must be taken to maintain the blood and nerve supplies. A long section of the sciatic nerve is cleared of connective tissue, and severed proximally. All branches of the distal stump that do not innervate the medial gastrocnemius muscle are severed. The distal nerve stump is inserted into a cuff lined with stainless steel stimulating wires. The calcaneus is severed, leaving a small piece of bone still attached to the Achilles tendon. Sonometric crystals and/or electrodes for electromyography can be inserted. Immobilization by metal probes in the femur and tibia prevents movement of the muscle origin. The Achilles tendon is attached to the force transducer and the loosened skin is pulled up at the sides to form a container that is filled with warmed paraffin oil. The oil distributes heat evenly and minimizes evaporative heat loss. A heat lamp is directed on the muscle, and the muscle and rat are allowed to warm up to 37°C. While it is warming, maximal voltage and optimal length can be determined. These are important initial conditions for any experiment on intact whole muscle. The experiment may include determination of standard contractile properties, like the force-frequency relationship, force-length relationship, and force-velocity relationship. With care in surgical isolation, immobilization of the origin of the muscle and alignment of the muscle-tendon unit with the force transducer, and proper data analysis, high quality measurements can be obtained with this muscle preparation. MyJove Corporation 2011-10-15 /pmc/articles/PMC3227207/ /pubmed/22025076 http://dx.doi.org/10.3791/3167 Text en Copyright © 2011, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Physiology MacIntosh, Brian R. Esau, Shane P. Holash, R. John Fletcher, Jared R. Procedures for Rat in situ Skeletal Muscle Contractile Properties |
title | Procedures for Rat in situ Skeletal Muscle Contractile Properties |
title_full | Procedures for Rat in situ Skeletal Muscle Contractile Properties |
title_fullStr | Procedures for Rat in situ Skeletal Muscle Contractile Properties |
title_full_unstemmed | Procedures for Rat in situ Skeletal Muscle Contractile Properties |
title_short | Procedures for Rat in situ Skeletal Muscle Contractile Properties |
title_sort | procedures for rat in situ skeletal muscle contractile properties |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3227207/ https://www.ncbi.nlm.nih.gov/pubmed/22025076 http://dx.doi.org/10.3791/3167 |
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