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Comparative genomic analysis of Streptococcus suis reveals significant genomic diversity among different serotypes

BACKGROUND: Streptococcus suis (S. suis) is a major swine pathogen and an emerging zoonotic agent. Serotypes 1, 2, 3, 7, 9, 14 and 1/2 are the most prevalent serotypes of this pathogen. However, almost all studies were carried out on serotype 2 strains. Therefore, characterization of genomic feature...

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Autores principales: Zhang, Anding, Yang, Ming, Hu, Pan, Wu, Jiayan, Chen, Bo, Hua, Yafeng, Yu, Jun, Chen, Huanchun, Xiao, Jingfa, Jin, Meilin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3227697/
https://www.ncbi.nlm.nih.gov/pubmed/22026465
http://dx.doi.org/10.1186/1471-2164-12-523
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author Zhang, Anding
Yang, Ming
Hu, Pan
Wu, Jiayan
Chen, Bo
Hua, Yafeng
Yu, Jun
Chen, Huanchun
Xiao, Jingfa
Jin, Meilin
author_facet Zhang, Anding
Yang, Ming
Hu, Pan
Wu, Jiayan
Chen, Bo
Hua, Yafeng
Yu, Jun
Chen, Huanchun
Xiao, Jingfa
Jin, Meilin
author_sort Zhang, Anding
collection PubMed
description BACKGROUND: Streptococcus suis (S. suis) is a major swine pathogen and an emerging zoonotic agent. Serotypes 1, 2, 3, 7, 9, 14 and 1/2 are the most prevalent serotypes of this pathogen. However, almost all studies were carried out on serotype 2 strains. Therefore, characterization of genomic features of other serotypes will be required to better understand their virulence potential and phylogenetic relationships among different serotypes. RESULTS: Four Chinese S. suis strains belonging to serotypes 1, 7, 9 and 1/2 were sequenced using a rapid, high-throughput approach. Based on the 13 corresponding serotype strains, including 9 previously completed genomes of this bacterium, a full comparative genomic analysis was performed. The results provide evidence that (i) the pan-genome of this species is open and the size increases with addition of new sequenced genomes, (ii) strains of serotypes 1, 3, 7 and 9 are phylogenetically distinct from serotype 2 strains, but all serotype 2 strains, plus the serotype 1/2 and 14 strains, are very closely related. (iii) all these strains, except for the serotype 1 strain, could harbor a recombinant site for a pathogenic island (89 K) mediated by conjugal transfer, and may have the ability to gain the 89 K sequence. CONCLUSIONS: There is significant genomic diversity among different strains in S. suis, and the gain and loss of large amount of genes are involved in shaping their genomes. This is indicated by (i) pairwise gene content comparisons between every pair of these strains, (ii) the open pan-genome of this species, (iii) the observed indels, invertions and rearrangements in the collinearity analysis. Phylogenetic relationships may be associated with serotype, as serotype 2 strains are closely related and distinct from other serotypes like 1, 3, 7 and 9, but more strains need to be sequenced to confirm this.
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spelling pubmed-32276972011-12-01 Comparative genomic analysis of Streptococcus suis reveals significant genomic diversity among different serotypes Zhang, Anding Yang, Ming Hu, Pan Wu, Jiayan Chen, Bo Hua, Yafeng Yu, Jun Chen, Huanchun Xiao, Jingfa Jin, Meilin BMC Genomics Research Article BACKGROUND: Streptococcus suis (S. suis) is a major swine pathogen and an emerging zoonotic agent. Serotypes 1, 2, 3, 7, 9, 14 and 1/2 are the most prevalent serotypes of this pathogen. However, almost all studies were carried out on serotype 2 strains. Therefore, characterization of genomic features of other serotypes will be required to better understand their virulence potential and phylogenetic relationships among different serotypes. RESULTS: Four Chinese S. suis strains belonging to serotypes 1, 7, 9 and 1/2 were sequenced using a rapid, high-throughput approach. Based on the 13 corresponding serotype strains, including 9 previously completed genomes of this bacterium, a full comparative genomic analysis was performed. The results provide evidence that (i) the pan-genome of this species is open and the size increases with addition of new sequenced genomes, (ii) strains of serotypes 1, 3, 7 and 9 are phylogenetically distinct from serotype 2 strains, but all serotype 2 strains, plus the serotype 1/2 and 14 strains, are very closely related. (iii) all these strains, except for the serotype 1 strain, could harbor a recombinant site for a pathogenic island (89 K) mediated by conjugal transfer, and may have the ability to gain the 89 K sequence. CONCLUSIONS: There is significant genomic diversity among different strains in S. suis, and the gain and loss of large amount of genes are involved in shaping their genomes. This is indicated by (i) pairwise gene content comparisons between every pair of these strains, (ii) the open pan-genome of this species, (iii) the observed indels, invertions and rearrangements in the collinearity analysis. Phylogenetic relationships may be associated with serotype, as serotype 2 strains are closely related and distinct from other serotypes like 1, 3, 7 and 9, but more strains need to be sequenced to confirm this. BioMed Central 2011-10-25 /pmc/articles/PMC3227697/ /pubmed/22026465 http://dx.doi.org/10.1186/1471-2164-12-523 Text en Copyright ©2011 Zhang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Anding
Yang, Ming
Hu, Pan
Wu, Jiayan
Chen, Bo
Hua, Yafeng
Yu, Jun
Chen, Huanchun
Xiao, Jingfa
Jin, Meilin
Comparative genomic analysis of Streptococcus suis reveals significant genomic diversity among different serotypes
title Comparative genomic analysis of Streptococcus suis reveals significant genomic diversity among different serotypes
title_full Comparative genomic analysis of Streptococcus suis reveals significant genomic diversity among different serotypes
title_fullStr Comparative genomic analysis of Streptococcus suis reveals significant genomic diversity among different serotypes
title_full_unstemmed Comparative genomic analysis of Streptococcus suis reveals significant genomic diversity among different serotypes
title_short Comparative genomic analysis of Streptococcus suis reveals significant genomic diversity among different serotypes
title_sort comparative genomic analysis of streptococcus suis reveals significant genomic diversity among different serotypes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3227697/
https://www.ncbi.nlm.nih.gov/pubmed/22026465
http://dx.doi.org/10.1186/1471-2164-12-523
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