Rapid empirical discovery of optimal peptides for targeted proteomics

We report a method for high-throughput, cost-efficient empirical discovery of optimal proteotypic peptides and fragment ions for targeted proteomics applications using in vitro-synthesized proteins. We demonstrate the approach using human transcription factors – which are typically difficult, low-ab...

Descripción completa

Detalles Bibliográficos
Autores principales: Stergachis, Andrew B., MacLean, Brendan, Lee, Kristen, Stamatoyannopoulos, John A., MacCoss, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3227787/
https://www.ncbi.nlm.nih.gov/pubmed/22056677
http://dx.doi.org/10.1038/nmeth.1770

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3227787/
https://www.ncbi.nlm.nih.gov/pubmed/22056677
http://dx.doi.org/10.1038/nmeth.1770

Ejemplares similares