Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves

We investigated the antiplasmodial properties of crude extracts from Carica papaya leaves to trace the activity through bioassay-guided fractionation. The greatest antiplasmodial activity was observed in the ethyl acetate crude extract. C. papaya showed a high selectivity for P. falciparum against C...

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Detalles Bibliográficos
Autores principales: Melariri, Paula, Campbell, William, Etusim, Paschal, Smith, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228370/
https://www.ncbi.nlm.nih.gov/pubmed/22174990
http://dx.doi.org/10.1155/2011/104954
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author Melariri, Paula
Campbell, William
Etusim, Paschal
Smith, Peter
author_facet Melariri, Paula
Campbell, William
Etusim, Paschal
Smith, Peter
author_sort Melariri, Paula
collection PubMed
description We investigated the antiplasmodial properties of crude extracts from Carica papaya leaves to trace the activity through bioassay-guided fractionation. The greatest antiplasmodial activity was observed in the ethyl acetate crude extract. C. papaya showed a high selectivity for P. falciparum against CHO cells with a selectivity index of 249.25 and 185.37 in the chloroquine-sensitive D10 and chloroquine-resistant DD2 strains, respectively. Carica papaya ethyl acetate extract was subjected to bioassay-guided fractionation to ascertain the most active fraction, which was purified and identified using high-pressure liquid chromatography (HPLC) and GC-MS (Gas chromatography-Mass spectrometry) methods. Linoleic and linolenic acids identified from the ethyl acetate fraction showed IC(50) of 6.88 μg/ml and 3.58 μg/ml, respectively. The study demonstrated greater antiplasmodial activity of the crude ethyl acetate extract of Carica papaya leaves with an IC(50) of 2.96 ± 0.14 μg/ml when compared to the activity of the fractions and isolated compounds.
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spelling pubmed-32283702011-12-15 Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves Melariri, Paula Campbell, William Etusim, Paschal Smith, Peter J Parasitol Res Research Article We investigated the antiplasmodial properties of crude extracts from Carica papaya leaves to trace the activity through bioassay-guided fractionation. The greatest antiplasmodial activity was observed in the ethyl acetate crude extract. C. papaya showed a high selectivity for P. falciparum against CHO cells with a selectivity index of 249.25 and 185.37 in the chloroquine-sensitive D10 and chloroquine-resistant DD2 strains, respectively. Carica papaya ethyl acetate extract was subjected to bioassay-guided fractionation to ascertain the most active fraction, which was purified and identified using high-pressure liquid chromatography (HPLC) and GC-MS (Gas chromatography-Mass spectrometry) methods. Linoleic and linolenic acids identified from the ethyl acetate fraction showed IC(50) of 6.88 μg/ml and 3.58 μg/ml, respectively. The study demonstrated greater antiplasmodial activity of the crude ethyl acetate extract of Carica papaya leaves with an IC(50) of 2.96 ± 0.14 μg/ml when compared to the activity of the fractions and isolated compounds. Hindawi Publishing Corporation 2011 2011-11-17 /pmc/articles/PMC3228370/ /pubmed/22174990 http://dx.doi.org/10.1155/2011/104954 Text en Copyright © 2011 Paula Melariri et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Melariri, Paula
Campbell, William
Etusim, Paschal
Smith, Peter
Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves
title Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves
title_full Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves
title_fullStr Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves
title_full_unstemmed Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves
title_short Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves
title_sort antiplasmodial properties and bioassay-guided fractionation of ethyl acetate extracts from carica papaya leaves
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228370/
https://www.ncbi.nlm.nih.gov/pubmed/22174990
http://dx.doi.org/10.1155/2011/104954
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