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Reduced cytotoxicity of insulin-immobilized CdS quantum dots using PEG as a spacer
Cytotoxicity is a severe problem for cadmium sulfide nanoparticles (CSNPs) in biological systems. In this study, mercaptoacetic acid-coated CSNPs, typical semiconductor Q-dots, were synthesized in aqueous medium by the arrested precipitation method. Then, amino-terminated polyethylene glycol (PEG) w...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228607/ https://www.ncbi.nlm.nih.gov/pubmed/21943314 http://dx.doi.org/10.1186/1556-276X-6-528 |
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author | Selim, KM Kamruzzaman Xing, Zhi-Cai Choi, Moon-Jeong Chang, Yongmin Guo, Haiqing Kang, Inn-Kyu |
author_facet | Selim, KM Kamruzzaman Xing, Zhi-Cai Choi, Moon-Jeong Chang, Yongmin Guo, Haiqing Kang, Inn-Kyu |
author_sort | Selim, KM Kamruzzaman |
collection | PubMed |
description | Cytotoxicity is a severe problem for cadmium sulfide nanoparticles (CSNPs) in biological systems. In this study, mercaptoacetic acid-coated CSNPs, typical semiconductor Q-dots, were synthesized in aqueous medium by the arrested precipitation method. Then, amino-terminated polyethylene glycol (PEG) was conjugated to the surface of CSNPs (PCSNPs) in order to introduce amino groups to the surface. Finally, insulin was immobilized on the surface of PCSNPs (ICSNPs) to reduce cytotoxicity as well as to enhance cell compatibility. The presence of insulin on the surface of ICSNPs was confirmed by observing infrared absorptions of amide I and II. The mean diameter of ICSNPs as determined by dynamic light scattering was about 38 nm. Human fibroblasts were cultured in the absence and presence of cadmium sulfide nanoparticles to evaluate cytotoxicity and cell compatibility. The results showed that the cytotoxicity of insulin-immobilized cadmium sulfide nanoparticles was significantly suppressed by usage of PEG as a spacer. In addition, cell proliferation was highly facilitated by the addition of ICSNPs. The ICSNPs used in this study will be potentials to be used in bio-imaging applications. |
format | Online Article Text |
id | pubmed-3228607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Springer |
record_format | MEDLINE/PubMed |
spelling | pubmed-32286072011-12-05 Reduced cytotoxicity of insulin-immobilized CdS quantum dots using PEG as a spacer Selim, KM Kamruzzaman Xing, Zhi-Cai Choi, Moon-Jeong Chang, Yongmin Guo, Haiqing Kang, Inn-Kyu Nanoscale Res Lett Nano Express Cytotoxicity is a severe problem for cadmium sulfide nanoparticles (CSNPs) in biological systems. In this study, mercaptoacetic acid-coated CSNPs, typical semiconductor Q-dots, were synthesized in aqueous medium by the arrested precipitation method. Then, amino-terminated polyethylene glycol (PEG) was conjugated to the surface of CSNPs (PCSNPs) in order to introduce amino groups to the surface. Finally, insulin was immobilized on the surface of PCSNPs (ICSNPs) to reduce cytotoxicity as well as to enhance cell compatibility. The presence of insulin on the surface of ICSNPs was confirmed by observing infrared absorptions of amide I and II. The mean diameter of ICSNPs as determined by dynamic light scattering was about 38 nm. Human fibroblasts were cultured in the absence and presence of cadmium sulfide nanoparticles to evaluate cytotoxicity and cell compatibility. The results showed that the cytotoxicity of insulin-immobilized cadmium sulfide nanoparticles was significantly suppressed by usage of PEG as a spacer. In addition, cell proliferation was highly facilitated by the addition of ICSNPs. The ICSNPs used in this study will be potentials to be used in bio-imaging applications. Springer 2011-09-23 /pmc/articles/PMC3228607/ /pubmed/21943314 http://dx.doi.org/10.1186/1556-276X-6-528 Text en Copyright ©2011 Selim et al; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Nano Express Selim, KM Kamruzzaman Xing, Zhi-Cai Choi, Moon-Jeong Chang, Yongmin Guo, Haiqing Kang, Inn-Kyu Reduced cytotoxicity of insulin-immobilized CdS quantum dots using PEG as a spacer |
title | Reduced cytotoxicity of insulin-immobilized CdS quantum dots using PEG as a spacer |
title_full | Reduced cytotoxicity of insulin-immobilized CdS quantum dots using PEG as a spacer |
title_fullStr | Reduced cytotoxicity of insulin-immobilized CdS quantum dots using PEG as a spacer |
title_full_unstemmed | Reduced cytotoxicity of insulin-immobilized CdS quantum dots using PEG as a spacer |
title_short | Reduced cytotoxicity of insulin-immobilized CdS quantum dots using PEG as a spacer |
title_sort | reduced cytotoxicity of insulin-immobilized cds quantum dots using peg as a spacer |
topic | Nano Express |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228607/ https://www.ncbi.nlm.nih.gov/pubmed/21943314 http://dx.doi.org/10.1186/1556-276X-6-528 |
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