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Obscurin Depletion Impairs Organization of Skeletal Muscle in Developing Zebrafish Embryos
During development, skeletal myoblasts differentiate into myocytes and skeletal myotubes with mature contractile structures that are precisely oriented with respect to surrounding cells and tissues. Establishment of this highly ordered structure requires reciprocal interactions between the different...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228690/ https://www.ncbi.nlm.nih.gov/pubmed/22190853 http://dx.doi.org/10.1155/2011/479135 |
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author | Raeker, Maide Ö. Russell, Mark W. |
author_facet | Raeker, Maide Ö. Russell, Mark W. |
author_sort | Raeker, Maide Ö. |
collection | PubMed |
description | During development, skeletal myoblasts differentiate into myocytes and skeletal myotubes with mature contractile structures that are precisely oriented with respect to surrounding cells and tissues. Establishment of this highly ordered structure requires reciprocal interactions between the differentiating myocytes and the surrounding extracellular matrix to form correctly positioned and well-organized attachments from the skeletal muscle to the bony skeleton. Using the developing zebrafish embryo as a model, we examined the relationship between new myofibril assembly and the organization of the membrane domains involved in cell-extracellular matrix interactions. We determined that depletion of obscurin, a giant muscle protein, resulted in irregular cell morphology and disturbed extracellular matrix organization during skeletal muscle development. The resulting impairment of myocyte organization was associated with disturbance of the internal architecture of the myocyte suggesting that obscurin participates in organizing the internal structure of the myocyte and translating those structural cues to surrounding cells and tissues. |
format | Online Article Text |
id | pubmed-3228690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-32286902011-12-21 Obscurin Depletion Impairs Organization of Skeletal Muscle in Developing Zebrafish Embryos Raeker, Maide Ö. Russell, Mark W. J Biomed Biotechnol Research Article During development, skeletal myoblasts differentiate into myocytes and skeletal myotubes with mature contractile structures that are precisely oriented with respect to surrounding cells and tissues. Establishment of this highly ordered structure requires reciprocal interactions between the differentiating myocytes and the surrounding extracellular matrix to form correctly positioned and well-organized attachments from the skeletal muscle to the bony skeleton. Using the developing zebrafish embryo as a model, we examined the relationship between new myofibril assembly and the organization of the membrane domains involved in cell-extracellular matrix interactions. We determined that depletion of obscurin, a giant muscle protein, resulted in irregular cell morphology and disturbed extracellular matrix organization during skeletal muscle development. The resulting impairment of myocyte organization was associated with disturbance of the internal architecture of the myocyte suggesting that obscurin participates in organizing the internal structure of the myocyte and translating those structural cues to surrounding cells and tissues. Hindawi Publishing Corporation 2011 2011-11-15 /pmc/articles/PMC3228690/ /pubmed/22190853 http://dx.doi.org/10.1155/2011/479135 Text en Copyright © 2011 M. Ö. Raeker and M. W. Russell. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Raeker, Maide Ö. Russell, Mark W. Obscurin Depletion Impairs Organization of Skeletal Muscle in Developing Zebrafish Embryos |
title | Obscurin Depletion Impairs Organization of Skeletal Muscle in Developing Zebrafish Embryos |
title_full | Obscurin Depletion Impairs Organization of Skeletal Muscle in Developing Zebrafish Embryos |
title_fullStr | Obscurin Depletion Impairs Organization of Skeletal Muscle in Developing Zebrafish Embryos |
title_full_unstemmed | Obscurin Depletion Impairs Organization of Skeletal Muscle in Developing Zebrafish Embryos |
title_short | Obscurin Depletion Impairs Organization of Skeletal Muscle in Developing Zebrafish Embryos |
title_sort | obscurin depletion impairs organization of skeletal muscle in developing zebrafish embryos |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228690/ https://www.ncbi.nlm.nih.gov/pubmed/22190853 http://dx.doi.org/10.1155/2011/479135 |
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