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Aquaporin-5: A Marker Protein for Proliferation and Migration of Human Breast Cancer Cells

Aquaporin (AQP) is a family of transmembrane proteins for water transport. Recent studies revealed that AQPs are likely to play a role in tumor progression and invasion. We aimed to examine the potential role of AQP5 in the progression of human breast cancer cells. Expression of AQP5 mRNA and protei...

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Autores principales: Jung, Hyun Jun, Park, Ji-Young, Jeon, Hyo-Sung, Kwon, Tae-Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228775/
https://www.ncbi.nlm.nih.gov/pubmed/22145049
http://dx.doi.org/10.1371/journal.pone.0028492
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author Jung, Hyun Jun
Park, Ji-Young
Jeon, Hyo-Sung
Kwon, Tae-Hwan
author_facet Jung, Hyun Jun
Park, Ji-Young
Jeon, Hyo-Sung
Kwon, Tae-Hwan
author_sort Jung, Hyun Jun
collection PubMed
description Aquaporin (AQP) is a family of transmembrane proteins for water transport. Recent studies revealed that AQPs are likely to play a role in tumor progression and invasion. We aimed to examine the potential role of AQP5 in the progression of human breast cancer cells. Expression of AQP5 mRNA and protein was seen in human breast cancer cell line (both MCF7 and MDA-MB-231) by RT-PCR and immunoblotting analysis. Immunoperoxidase labeling of AQP5 was observed at ductal epithelial cells of human breast tissues. In benign tumor, AQP5 labeling was mainly seen at the apical domains of ductal epithelial cells. In contrast, in invasive ductal carcinoma, prominent AQP5 labeling was associated with cancer cells, whereas some ducts were unlabeled and apical polarity of AQP5 in ducts was lost. Cell proliferation (BrdU incorporation assay) and migration of MCF7 cells were significantly attenuated by lentivirus-mediated AQP5-shRNA transduction. Hyperosmotic stress induced by sorbitol treatment (100 mM, 24 h) reduced AQP5 expression in MCF7 cells, which was also associated with a significant reduction in cell proliferation and migration. Taken together, prominent AQP5 expression in breast cancer cells with the loss of polarity of ductal epithelial cells was seen during the progression of breast carcinoma. shRNA- or hyperosmotic stress-induced reduction in AQP5 expression of MCF7 cells was associated with significantly reduced cell proliferation and migration. In conclusion, AQP5 overexpression is likely to play a role in cell growth and metastasis of human breast cancer and could be a novel target for anti-breast cancer treatment.
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spelling pubmed-32287752011-12-05 Aquaporin-5: A Marker Protein for Proliferation and Migration of Human Breast Cancer Cells Jung, Hyun Jun Park, Ji-Young Jeon, Hyo-Sung Kwon, Tae-Hwan PLoS One Research Article Aquaporin (AQP) is a family of transmembrane proteins for water transport. Recent studies revealed that AQPs are likely to play a role in tumor progression and invasion. We aimed to examine the potential role of AQP5 in the progression of human breast cancer cells. Expression of AQP5 mRNA and protein was seen in human breast cancer cell line (both MCF7 and MDA-MB-231) by RT-PCR and immunoblotting analysis. Immunoperoxidase labeling of AQP5 was observed at ductal epithelial cells of human breast tissues. In benign tumor, AQP5 labeling was mainly seen at the apical domains of ductal epithelial cells. In contrast, in invasive ductal carcinoma, prominent AQP5 labeling was associated with cancer cells, whereas some ducts were unlabeled and apical polarity of AQP5 in ducts was lost. Cell proliferation (BrdU incorporation assay) and migration of MCF7 cells were significantly attenuated by lentivirus-mediated AQP5-shRNA transduction. Hyperosmotic stress induced by sorbitol treatment (100 mM, 24 h) reduced AQP5 expression in MCF7 cells, which was also associated with a significant reduction in cell proliferation and migration. Taken together, prominent AQP5 expression in breast cancer cells with the loss of polarity of ductal epithelial cells was seen during the progression of breast carcinoma. shRNA- or hyperosmotic stress-induced reduction in AQP5 expression of MCF7 cells was associated with significantly reduced cell proliferation and migration. In conclusion, AQP5 overexpression is likely to play a role in cell growth and metastasis of human breast cancer and could be a novel target for anti-breast cancer treatment. Public Library of Science 2011-12-01 /pmc/articles/PMC3228775/ /pubmed/22145049 http://dx.doi.org/10.1371/journal.pone.0028492 Text en Jung et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jung, Hyun Jun
Park, Ji-Young
Jeon, Hyo-Sung
Kwon, Tae-Hwan
Aquaporin-5: A Marker Protein for Proliferation and Migration of Human Breast Cancer Cells
title Aquaporin-5: A Marker Protein for Proliferation and Migration of Human Breast Cancer Cells
title_full Aquaporin-5: A Marker Protein for Proliferation and Migration of Human Breast Cancer Cells
title_fullStr Aquaporin-5: A Marker Protein for Proliferation and Migration of Human Breast Cancer Cells
title_full_unstemmed Aquaporin-5: A Marker Protein for Proliferation and Migration of Human Breast Cancer Cells
title_short Aquaporin-5: A Marker Protein for Proliferation and Migration of Human Breast Cancer Cells
title_sort aquaporin-5: a marker protein for proliferation and migration of human breast cancer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228775/
https://www.ncbi.nlm.nih.gov/pubmed/22145049
http://dx.doi.org/10.1371/journal.pone.0028492
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