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Feeding Cells Induced by Phytoparasitic Nematodes Require γ-Tubulin Ring Complex for Microtubule Reorganization
Reorganization of the microtubule network is important for the fast isodiametric expansion of giant-feeding cells induced by root-knot nematodes. The efficiency of microtubule reorganization depends on the nucleation of new microtubules, their elongation rate and activity of microtubule severing fac...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228788/ https://www.ncbi.nlm.nih.gov/pubmed/22144887 http://dx.doi.org/10.1371/journal.ppat.1002343 |
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author | Banora, Mohamed Youssef Rodiuc, Natalia Baldacci-Cresp, Fabien Smertenko, Andrei Bleve-Zacheo, Teresa Mellilo, Maria Teresa Karimi, Mansour Hilson, Pierre Evrard, Jean-Luc Favery, Bruno Engler, Gilbert Abad, Pierre de Almeida Engler, Janice |
author_facet | Banora, Mohamed Youssef Rodiuc, Natalia Baldacci-Cresp, Fabien Smertenko, Andrei Bleve-Zacheo, Teresa Mellilo, Maria Teresa Karimi, Mansour Hilson, Pierre Evrard, Jean-Luc Favery, Bruno Engler, Gilbert Abad, Pierre de Almeida Engler, Janice |
author_sort | Banora, Mohamed Youssef |
collection | PubMed |
description | Reorganization of the microtubule network is important for the fast isodiametric expansion of giant-feeding cells induced by root-knot nematodes. The efficiency of microtubule reorganization depends on the nucleation of new microtubules, their elongation rate and activity of microtubule severing factors. New microtubules in plants are nucleated by cytoplasmic or microtubule-bound γ-tubulin ring complexes. Here we investigate the requirement of γ-tubulin complexes for giant feeding cells development using the interaction between Arabidopsis and Meloidogyne spp. as a model system. Immunocytochemical analyses demonstrate that γ-tubulin localizes to both cortical cytoplasm and mitotic microtubule arrays of the giant cells where it can associate with microtubules. The transcripts of two Arabidopsis γ-tubulin (TUBG1 and TUBG2) and two γ-tubulin complex proteins genes (GCP3 and GCP4) are upregulated in galls. Electron microscopy demonstrates association of GCP3 and γ-tubulin as part of a complex in the cytoplasm of giant cells. Knockout of either or both γ-tubulin genes results in the gene dose-dependent alteration of the morphology of feeding site and failure of nematode life cycle completion. We conclude that the γ-tubulin complex is essential for the control of microtubular network remodelling in the course of initiation and development of giant-feeding cells, and for the successful reproduction of nematodes in their plant hosts. |
format | Online Article Text |
id | pubmed-3228788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32287882011-12-05 Feeding Cells Induced by Phytoparasitic Nematodes Require γ-Tubulin Ring Complex for Microtubule Reorganization Banora, Mohamed Youssef Rodiuc, Natalia Baldacci-Cresp, Fabien Smertenko, Andrei Bleve-Zacheo, Teresa Mellilo, Maria Teresa Karimi, Mansour Hilson, Pierre Evrard, Jean-Luc Favery, Bruno Engler, Gilbert Abad, Pierre de Almeida Engler, Janice PLoS Pathog Research Article Reorganization of the microtubule network is important for the fast isodiametric expansion of giant-feeding cells induced by root-knot nematodes. The efficiency of microtubule reorganization depends on the nucleation of new microtubules, their elongation rate and activity of microtubule severing factors. New microtubules in plants are nucleated by cytoplasmic or microtubule-bound γ-tubulin ring complexes. Here we investigate the requirement of γ-tubulin complexes for giant feeding cells development using the interaction between Arabidopsis and Meloidogyne spp. as a model system. Immunocytochemical analyses demonstrate that γ-tubulin localizes to both cortical cytoplasm and mitotic microtubule arrays of the giant cells where it can associate with microtubules. The transcripts of two Arabidopsis γ-tubulin (TUBG1 and TUBG2) and two γ-tubulin complex proteins genes (GCP3 and GCP4) are upregulated in galls. Electron microscopy demonstrates association of GCP3 and γ-tubulin as part of a complex in the cytoplasm of giant cells. Knockout of either or both γ-tubulin genes results in the gene dose-dependent alteration of the morphology of feeding site and failure of nematode life cycle completion. We conclude that the γ-tubulin complex is essential for the control of microtubular network remodelling in the course of initiation and development of giant-feeding cells, and for the successful reproduction of nematodes in their plant hosts. Public Library of Science 2011-12-01 /pmc/articles/PMC3228788/ /pubmed/22144887 http://dx.doi.org/10.1371/journal.ppat.1002343 Text en Youssef Banora et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Banora, Mohamed Youssef Rodiuc, Natalia Baldacci-Cresp, Fabien Smertenko, Andrei Bleve-Zacheo, Teresa Mellilo, Maria Teresa Karimi, Mansour Hilson, Pierre Evrard, Jean-Luc Favery, Bruno Engler, Gilbert Abad, Pierre de Almeida Engler, Janice Feeding Cells Induced by Phytoparasitic Nematodes Require γ-Tubulin Ring Complex for Microtubule Reorganization |
title | Feeding Cells Induced by Phytoparasitic Nematodes Require γ-Tubulin Ring Complex for Microtubule Reorganization |
title_full | Feeding Cells Induced by Phytoparasitic Nematodes Require γ-Tubulin Ring Complex for Microtubule Reorganization |
title_fullStr | Feeding Cells Induced by Phytoparasitic Nematodes Require γ-Tubulin Ring Complex for Microtubule Reorganization |
title_full_unstemmed | Feeding Cells Induced by Phytoparasitic Nematodes Require γ-Tubulin Ring Complex for Microtubule Reorganization |
title_short | Feeding Cells Induced by Phytoparasitic Nematodes Require γ-Tubulin Ring Complex for Microtubule Reorganization |
title_sort | feeding cells induced by phytoparasitic nematodes require γ-tubulin ring complex for microtubule reorganization |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228788/ https://www.ncbi.nlm.nih.gov/pubmed/22144887 http://dx.doi.org/10.1371/journal.ppat.1002343 |
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