Embryonic stem cell-based system for mapping developmental transcriptional programs

Study of developmentally regulated transcription factors by chromatin immunoprecipitation and sequencing (ChIP-seq) faces two major obstacles: availability of ChIP grade antibodies and access to sufficient number of cells. We describe a versatile method for genome-wide analysis of transcription fact...

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Detalles Bibliográficos
Autores principales: Mazzoni, Esteban O., Mahony, Shaun, Iacovino, Michelina, Morrison, Carolyn A., Mountoufaris, George, Closser, Michael, Whyte, Warren A., Young, Richard A., Kyba, Michael, Gifford, David K., Wichterle, Hynek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228994/
https://www.ncbi.nlm.nih.gov/pubmed/22081127
http://dx.doi.org/10.1038/nmeth.1775
Descripción
Sumario:Study of developmentally regulated transcription factors by chromatin immunoprecipitation and sequencing (ChIP-seq) faces two major obstacles: availability of ChIP grade antibodies and access to sufficient number of cells. We describe a versatile method for genome-wide analysis of transcription factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping transcription factors involved in motor neuron specification.

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