Embryonic stem cell-based system for mapping developmental transcriptional programs

Study of developmentally regulated transcription factors by chromatin immunoprecipitation and sequencing (ChIP-seq) faces two major obstacles: availability of ChIP grade antibodies and access to sufficient number of cells. We describe a versatile method for genome-wide analysis of transcription fact...

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Autores principales: Mazzoni, Esteban O., Mahony, Shaun, Iacovino, Michelina, Morrison, Carolyn A., Mountoufaris, George, Closser, Michael, Whyte, Warren A., Young, Richard A., Kyba, Michael, Gifford, David K., Wichterle, Hynek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228994/
https://www.ncbi.nlm.nih.gov/pubmed/22081127
http://dx.doi.org/10.1038/nmeth.1775
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author Mazzoni, Esteban O.
Mahony, Shaun
Iacovino, Michelina
Morrison, Carolyn A.
Mountoufaris, George
Closser, Michael
Whyte, Warren A.
Young, Richard A.
Kyba, Michael
Gifford, David K.
Wichterle, Hynek
author_facet Mazzoni, Esteban O.
Mahony, Shaun
Iacovino, Michelina
Morrison, Carolyn A.
Mountoufaris, George
Closser, Michael
Whyte, Warren A.
Young, Richard A.
Kyba, Michael
Gifford, David K.
Wichterle, Hynek
author_sort Mazzoni, Esteban O.
collection PubMed
description Study of developmentally regulated transcription factors by chromatin immunoprecipitation and sequencing (ChIP-seq) faces two major obstacles: availability of ChIP grade antibodies and access to sufficient number of cells. We describe a versatile method for genome-wide analysis of transcription factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping transcription factors involved in motor neuron specification.
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spelling pubmed-32289942012-06-01 Embryonic stem cell-based system for mapping developmental transcriptional programs Mazzoni, Esteban O. Mahony, Shaun Iacovino, Michelina Morrison, Carolyn A. Mountoufaris, George Closser, Michael Whyte, Warren A. Young, Richard A. Kyba, Michael Gifford, David K. Wichterle, Hynek Nat Methods Article Study of developmentally regulated transcription factors by chromatin immunoprecipitation and sequencing (ChIP-seq) faces two major obstacles: availability of ChIP grade antibodies and access to sufficient number of cells. We describe a versatile method for genome-wide analysis of transcription factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping transcription factors involved in motor neuron specification. 2011-11-13 /pmc/articles/PMC3228994/ /pubmed/22081127 http://dx.doi.org/10.1038/nmeth.1775 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Mazzoni, Esteban O.
Mahony, Shaun
Iacovino, Michelina
Morrison, Carolyn A.
Mountoufaris, George
Closser, Michael
Whyte, Warren A.
Young, Richard A.
Kyba, Michael
Gifford, David K.
Wichterle, Hynek
Embryonic stem cell-based system for mapping developmental transcriptional programs
title Embryonic stem cell-based system for mapping developmental transcriptional programs
title_full Embryonic stem cell-based system for mapping developmental transcriptional programs
title_fullStr Embryonic stem cell-based system for mapping developmental transcriptional programs
title_full_unstemmed Embryonic stem cell-based system for mapping developmental transcriptional programs
title_short Embryonic stem cell-based system for mapping developmental transcriptional programs
title_sort embryonic stem cell-based system for mapping developmental transcriptional programs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228994/
https://www.ncbi.nlm.nih.gov/pubmed/22081127
http://dx.doi.org/10.1038/nmeth.1775
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