Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids

Khat (Catha edulis Forsk.) is a flowering perennial shrub cultivated for its neurostimulant properties resulting mainly from the occurrence of (S)-cathinone in young leaves. The biosynthesis of (S)-cathinone and the related phenylpropylamino alkaloids (1S,2S)-cathine and (1R,2S)-norephedrine is not...

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Autores principales: Hagel, Jillian M., Krizevski, Raz, Kilpatrick, Korey, Sitrit, Yaron, Marsolais, Frédéric, Lewinsohn, Efraim, Facchini, Peter J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2011
Materias:
Plant Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229120/
https://www.ncbi.nlm.nih.gov/pubmed/22215969
http://dx.doi.org/10.1590/S1415-47572011000400017
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author Hagel, Jillian M.
Krizevski, Raz
Kilpatrick, Korey
Sitrit, Yaron
Marsolais, Frédéric
Lewinsohn, Efraim
Facchini, Peter J.
author_facet Hagel, Jillian M.
Krizevski, Raz
Kilpatrick, Korey
Sitrit, Yaron
Marsolais, Frédéric
Lewinsohn, Efraim
Facchini, Peter J.
author_sort Hagel, Jillian M.
collection PubMed
description Khat (Catha edulis Forsk.) is a flowering perennial shrub cultivated for its neurostimulant properties resulting mainly from the occurrence of (S)-cathinone in young leaves. The biosynthesis of (S)-cathinone and the related phenylpropylamino alkaloids (1S,2S)-cathine and (1R,2S)-norephedrine is not well characterized in plants. We prepared a cDNA library from young khat leaves and sequenced 4,896 random clones, generating an expressed sequence tag (EST) library of 3,293 unigenes. Putative functions were assigned to > 98% of the ESTs, providing a key resource for gene discovery. Candidates potentially involved at various stages of phenylpropylamino alkaloid biosynthesis from L-phenylalanine to (1S,2S)-cathine were identified.
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spelling pubmed-32291202012-01-03 Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids Hagel, Jillian M. Krizevski, Raz Kilpatrick, Korey Sitrit, Yaron Marsolais, Frédéric Lewinsohn, Efraim Facchini, Peter J. Genet Mol Biol Plant Genetics Khat (Catha edulis Forsk.) is a flowering perennial shrub cultivated for its neurostimulant properties resulting mainly from the occurrence of (S)-cathinone in young leaves. The biosynthesis of (S)-cathinone and the related phenylpropylamino alkaloids (1S,2S)-cathine and (1R,2S)-norephedrine is not well characterized in plants. We prepared a cDNA library from young khat leaves and sequenced 4,896 random clones, generating an expressed sequence tag (EST) library of 3,293 unigenes. Putative functions were assigned to > 98% of the ESTs, providing a key resource for gene discovery. Candidates potentially involved at various stages of phenylpropylamino alkaloid biosynthesis from L-phenylalanine to (1S,2S)-cathine were identified. Sociedade Brasileira de Genética 2011-10-01 2011 /pmc/articles/PMC3229120/ /pubmed/22215969 http://dx.doi.org/10.1590/S1415-47572011000400017 Text en Copyright © 2011, Sociedade Brasileira de Genética. Printed in Brazil License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Plant Genetics
Hagel, Jillian M.
Krizevski, Raz
Kilpatrick, Korey
Sitrit, Yaron
Marsolais, Frédéric
Lewinsohn, Efraim
Facchini, Peter J.
Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids
title Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids
title_full Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids
title_fullStr Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids
title_full_unstemmed Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids
title_short Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids
title_sort expressed sequence tag analysis of khat (catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids
topic Plant Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229120/
https://www.ncbi.nlm.nih.gov/pubmed/22215969
http://dx.doi.org/10.1590/S1415-47572011000400017
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