Optimizing expression and purification of an ATP-binding gene gsiA from Escherichia coli k-12 by using GFP fusion

The cloning, expression and purification of the glutathione (sulfur) import system ATP-binding protein (gsiA) was carried out. The coding sequence of Escherichia coli gsiA, which encodes the ATP-binding protein of a glutathione importer, was amplified by PCR, and then inserted into a prokaryotic exp...

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Detalles Bibliográficos
Autores principales: Wang, Zhongshan, Xiang, Quanju, Wang, Guangjun, Wang, Haiyan, Zhang, Yizheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2011
Materias:
Genetics of Microorganisms
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229122/
https://www.ncbi.nlm.nih.gov/pubmed/22215971
http://dx.doi.org/10.1590/S1415-47572011005000043

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