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Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene
Endophytic bacteria from three arboreal species native to the Amazon (Carapa guianenses, Ceiba pentandra, and Swietenia macrophylla), were isolated and identified, through partial sequencing of the 16S rRNA encoding gene. From these, 16 isolates were obtained, although, when compared to sequences de...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Sociedade Brasileira de Genética
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229124/ https://www.ncbi.nlm.nih.gov/pubmed/22215973 http://dx.doi.org/10.1590/S1415-47572011005000045 |
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author | Coêlho, Mariza M. Ferreira-Nozawa, Monica S. Nozawa, Sérgio R. Santos, André L.W. |
author_facet | Coêlho, Mariza M. Ferreira-Nozawa, Monica S. Nozawa, Sérgio R. Santos, André L.W. |
author_sort | Coêlho, Mariza M. |
collection | PubMed |
description | Endophytic bacteria from three arboreal species native to the Amazon (Carapa guianenses, Ceiba pentandra, and Swietenia macrophylla), were isolated and identified, through partial sequencing of the 16S rRNA encoding gene. From these, 16 isolates were obtained, although, when compared to sequences deposited in GenBank, only seven had produced identifiable fragments. Bacillus, Pantoea and two non-culturable samples were identified. Results obtained through sequence analysis revealed low genetic diversity across the isolates, even when analyzing different species and plant structures. This is the first report concerning the isolation and identification of endophytic bacteria in these plant species. |
format | Online Article Text |
id | pubmed-3229124 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-32291242012-01-03 Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene Coêlho, Mariza M. Ferreira-Nozawa, Monica S. Nozawa, Sérgio R. Santos, André L.W. Genet Mol Biol Genetics of Microorganisms Endophytic bacteria from three arboreal species native to the Amazon (Carapa guianenses, Ceiba pentandra, and Swietenia macrophylla), were isolated and identified, through partial sequencing of the 16S rRNA encoding gene. From these, 16 isolates were obtained, although, when compared to sequences deposited in GenBank, only seven had produced identifiable fragments. Bacillus, Pantoea and two non-culturable samples were identified. Results obtained through sequence analysis revealed low genetic diversity across the isolates, even when analyzing different species and plant structures. This is the first report concerning the isolation and identification of endophytic bacteria in these plant species. Sociedade Brasileira de Genética 2011-10-01 2011 /pmc/articles/PMC3229124/ /pubmed/22215973 http://dx.doi.org/10.1590/S1415-47572011005000045 Text en Copyright © 2011, Sociedade Brasileira de Genética. Printed in Brazil License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Genetics of Microorganisms Coêlho, Mariza M. Ferreira-Nozawa, Monica S. Nozawa, Sérgio R. Santos, André L.W. Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene |
title | Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene |
title_full | Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene |
title_fullStr | Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene |
title_full_unstemmed | Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene |
title_short | Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene |
title_sort | isolation of endophytic bacteria from arboreal species of the amazon and identification by sequencing of the 16s rrna encoding gene |
topic | Genetics of Microorganisms |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229124/ https://www.ncbi.nlm.nih.gov/pubmed/22215973 http://dx.doi.org/10.1590/S1415-47572011005000045 |
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