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Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene

Endophytic bacteria from three arboreal species native to the Amazon (Carapa guianenses, Ceiba pentandra, and Swietenia macrophylla), were isolated and identified, through partial sequencing of the 16S rRNA encoding gene. From these, 16 isolates were obtained, although, when compared to sequences de...

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Autores principales: Coêlho, Mariza M., Ferreira-Nozawa, Monica S., Nozawa, Sérgio R., Santos, André L.W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229124/
https://www.ncbi.nlm.nih.gov/pubmed/22215973
http://dx.doi.org/10.1590/S1415-47572011005000045
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author Coêlho, Mariza M.
Ferreira-Nozawa, Monica S.
Nozawa, Sérgio R.
Santos, André L.W.
author_facet Coêlho, Mariza M.
Ferreira-Nozawa, Monica S.
Nozawa, Sérgio R.
Santos, André L.W.
author_sort Coêlho, Mariza M.
collection PubMed
description Endophytic bacteria from three arboreal species native to the Amazon (Carapa guianenses, Ceiba pentandra, and Swietenia macrophylla), were isolated and identified, through partial sequencing of the 16S rRNA encoding gene. From these, 16 isolates were obtained, although, when compared to sequences deposited in GenBank, only seven had produced identifiable fragments. Bacillus, Pantoea and two non-culturable samples were identified. Results obtained through sequence analysis revealed low genetic diversity across the isolates, even when analyzing different species and plant structures. This is the first report concerning the isolation and identification of endophytic bacteria in these plant species.
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spelling pubmed-32291242012-01-03 Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene Coêlho, Mariza M. Ferreira-Nozawa, Monica S. Nozawa, Sérgio R. Santos, André L.W. Genet Mol Biol Genetics of Microorganisms Endophytic bacteria from three arboreal species native to the Amazon (Carapa guianenses, Ceiba pentandra, and Swietenia macrophylla), were isolated and identified, through partial sequencing of the 16S rRNA encoding gene. From these, 16 isolates were obtained, although, when compared to sequences deposited in GenBank, only seven had produced identifiable fragments. Bacillus, Pantoea and two non-culturable samples were identified. Results obtained through sequence analysis revealed low genetic diversity across the isolates, even when analyzing different species and plant structures. This is the first report concerning the isolation and identification of endophytic bacteria in these plant species. Sociedade Brasileira de Genética 2011-10-01 2011 /pmc/articles/PMC3229124/ /pubmed/22215973 http://dx.doi.org/10.1590/S1415-47572011005000045 Text en Copyright © 2011, Sociedade Brasileira de Genética. Printed in Brazil License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genetics of Microorganisms
Coêlho, Mariza M.
Ferreira-Nozawa, Monica S.
Nozawa, Sérgio R.
Santos, André L.W.
Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene
title Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene
title_full Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene
title_fullStr Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene
title_full_unstemmed Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene
title_short Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene
title_sort isolation of endophytic bacteria from arboreal species of the amazon and identification by sequencing of the 16s rrna encoding gene
topic Genetics of Microorganisms
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229124/
https://www.ncbi.nlm.nih.gov/pubmed/22215973
http://dx.doi.org/10.1590/S1415-47572011005000045
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