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Sulfated-Polysaccharide Fraction from Red Algae Gracilaria caudata Protects Mice Gut Against Ethanol-Induced Damage

The aim of the present study was to investigate the gastroprotective activity of a sulfated-polysaccharide (PLS) fraction extracted from the marine red algae Gracilaria caudata and the mechanism underlying the gastroprotective activity. Male Swiss mice were treated with PLS (3, 10, 30 and 90 mg·kg(−...

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Autores principales: Silva, Renan Oliveira, dos Santos, Geice Maria Pereira, Nicolau, Lucas Antonio Duarte, Lucetti, Larisse Tavares, Santana, Ana Paula Macedo, de Souza Chaves, Luciano, Barros, Francisco Clark Nogueira, Freitas, Ana Lúcia Ponte, Souza, Marcellus Henrique Loiola Ponte, Medeiros, Jand-Venes Rolim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229230/
https://www.ncbi.nlm.nih.gov/pubmed/22163181
http://dx.doi.org/10.3390/md9112188
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author Silva, Renan Oliveira
dos Santos, Geice Maria Pereira
Nicolau, Lucas Antonio Duarte
Lucetti, Larisse Tavares
Santana, Ana Paula Macedo
de Souza Chaves, Luciano
Barros, Francisco Clark Nogueira
Freitas, Ana Lúcia Ponte
Souza, Marcellus Henrique Loiola Ponte
Medeiros, Jand-Venes Rolim
author_facet Silva, Renan Oliveira
dos Santos, Geice Maria Pereira
Nicolau, Lucas Antonio Duarte
Lucetti, Larisse Tavares
Santana, Ana Paula Macedo
de Souza Chaves, Luciano
Barros, Francisco Clark Nogueira
Freitas, Ana Lúcia Ponte
Souza, Marcellus Henrique Loiola Ponte
Medeiros, Jand-Venes Rolim
author_sort Silva, Renan Oliveira
collection PubMed
description The aim of the present study was to investigate the gastroprotective activity of a sulfated-polysaccharide (PLS) fraction extracted from the marine red algae Gracilaria caudata and the mechanism underlying the gastroprotective activity. Male Swiss mice were treated with PLS (3, 10, 30 and 90 mg·kg(−1), p.o.), and after 30 min, they were administered 50% ethanol (0.5 mL/25 g(−1), p.o.). One hour later, gastric damage was measured using a planimeter. Samples of the stomach tissue were also obtained for histopathological assessment and for assays of glutathione (GSH) and malondialdehyde (MDA). Other groups were pretreated with l-NAME (10 mg·kg(−1), i.p.), dl-propargylglycine (PAG, 50 mg·kg(−1), p.o.) or glibenclamide (5 mg·kg(−1), i.p.). After 1 h, PLS (30 mg·kg(−1), p.o.) was administered. After 30 min, ethanol 50% was administered (0.5 mL/25g(−1), p.o.), followed by sacrifice after 60 min. PLS prevented-ethanol-induced macroscopic and microscopic gastric injury in a dose-dependent manner. However, treatment with l-NAME or glibenclamide reversed this gastroprotective effect. Administration of propargylglycine did not influence the effect of PLS. Our results suggest that PLS has a protective effect against ethanol-induced gastric damage in mice via activation of the NO/K(ATP) pathway.
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spelling pubmed-32292302011-12-12 Sulfated-Polysaccharide Fraction from Red Algae Gracilaria caudata Protects Mice Gut Against Ethanol-Induced Damage Silva, Renan Oliveira dos Santos, Geice Maria Pereira Nicolau, Lucas Antonio Duarte Lucetti, Larisse Tavares Santana, Ana Paula Macedo de Souza Chaves, Luciano Barros, Francisco Clark Nogueira Freitas, Ana Lúcia Ponte Souza, Marcellus Henrique Loiola Ponte Medeiros, Jand-Venes Rolim Mar Drugs Article The aim of the present study was to investigate the gastroprotective activity of a sulfated-polysaccharide (PLS) fraction extracted from the marine red algae Gracilaria caudata and the mechanism underlying the gastroprotective activity. Male Swiss mice were treated with PLS (3, 10, 30 and 90 mg·kg(−1), p.o.), and after 30 min, they were administered 50% ethanol (0.5 mL/25 g(−1), p.o.). One hour later, gastric damage was measured using a planimeter. Samples of the stomach tissue were also obtained for histopathological assessment and for assays of glutathione (GSH) and malondialdehyde (MDA). Other groups were pretreated with l-NAME (10 mg·kg(−1), i.p.), dl-propargylglycine (PAG, 50 mg·kg(−1), p.o.) or glibenclamide (5 mg·kg(−1), i.p.). After 1 h, PLS (30 mg·kg(−1), p.o.) was administered. After 30 min, ethanol 50% was administered (0.5 mL/25g(−1), p.o.), followed by sacrifice after 60 min. PLS prevented-ethanol-induced macroscopic and microscopic gastric injury in a dose-dependent manner. However, treatment with l-NAME or glibenclamide reversed this gastroprotective effect. Administration of propargylglycine did not influence the effect of PLS. Our results suggest that PLS has a protective effect against ethanol-induced gastric damage in mice via activation of the NO/K(ATP) pathway. Molecular Diversity Preservation International 2011-11-02 /pmc/articles/PMC3229230/ /pubmed/22163181 http://dx.doi.org/10.3390/md9112188 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Silva, Renan Oliveira
dos Santos, Geice Maria Pereira
Nicolau, Lucas Antonio Duarte
Lucetti, Larisse Tavares
Santana, Ana Paula Macedo
de Souza Chaves, Luciano
Barros, Francisco Clark Nogueira
Freitas, Ana Lúcia Ponte
Souza, Marcellus Henrique Loiola Ponte
Medeiros, Jand-Venes Rolim
Sulfated-Polysaccharide Fraction from Red Algae Gracilaria caudata Protects Mice Gut Against Ethanol-Induced Damage
title Sulfated-Polysaccharide Fraction from Red Algae Gracilaria caudata Protects Mice Gut Against Ethanol-Induced Damage
title_full Sulfated-Polysaccharide Fraction from Red Algae Gracilaria caudata Protects Mice Gut Against Ethanol-Induced Damage
title_fullStr Sulfated-Polysaccharide Fraction from Red Algae Gracilaria caudata Protects Mice Gut Against Ethanol-Induced Damage
title_full_unstemmed Sulfated-Polysaccharide Fraction from Red Algae Gracilaria caudata Protects Mice Gut Against Ethanol-Induced Damage
title_short Sulfated-Polysaccharide Fraction from Red Algae Gracilaria caudata Protects Mice Gut Against Ethanol-Induced Damage
title_sort sulfated-polysaccharide fraction from red algae gracilaria caudata protects mice gut against ethanol-induced damage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229230/
https://www.ncbi.nlm.nih.gov/pubmed/22163181
http://dx.doi.org/10.3390/md9112188
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