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Nonlinear changes in the activity of the oxygen-dependent demethylase system in Rhodococcus erythropolis cells in the presence of low and very low doses of formaldehyde

The effect of exogenous, highly diluted formaldehyde on the rate of demethylation/re-methylation of veratric acid by the bacteria Rhodococcus erythropolis was studied using electrophoretic and microscopic techniques. The activity of 4-O-demethylase, responsible for accumulation of vanillic acid, and...

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Autores principales: Malarczyk, Elżbieta, Pazdzioch-Czochra, Marzanna, Grąz, Marcin, Kochmańska-Rdest, Janina, Jarosz-Wilkołazka, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229444/
https://www.ncbi.nlm.nih.gov/pubmed/22104369
http://dx.doi.org/10.1186/1753-4631-5-9
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author Malarczyk, Elżbieta
Pazdzioch-Czochra, Marzanna
Grąz, Marcin
Kochmańska-Rdest, Janina
Jarosz-Wilkołazka, Anna
author_facet Malarczyk, Elżbieta
Pazdzioch-Czochra, Marzanna
Grąz, Marcin
Kochmańska-Rdest, Janina
Jarosz-Wilkołazka, Anna
author_sort Malarczyk, Elżbieta
collection PubMed
description The effect of exogenous, highly diluted formaldehyde on the rate of demethylation/re-methylation of veratric acid by the bacteria Rhodococcus erythropolis was studied using electrophoretic and microscopic techniques. The activity of 4-O-demethylase, responsible for accumulation of vanillic acid, and the levels of veratric and vanillic acids were determined using capillary electrophoresis. Formaldehyde was serially diluted at 1:100 ratios, and the total number of iterations was 20. After incubation of the successive dilutions of formaldehyde with the bacteria, demethylase activity oscillated in a sinusoidal manner. It was established using capillary electrophoresis that methylation of vanillic acid to veratric acid occurred at a double rate, as shown by the doubled fluctuation in the concentration of veratrate. There were also changes in the NADH oxidase activity, which is associated with methylation processes. Microscopic observations revealed the presence of numerous enlarged vacuoles in bacterial cells during the accumulation of large amounts of vanillic acid, and their disappearance together with a decrease in 4-O-demethylase activity. The presented results give evidence for the ability of living cells to detect the presence of submolecular concentrations of biological effectors in their environment and provide a basis for a scientific explanation of the law of hormesis and the therapeutic effect of homeopathic dilutions.
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spelling pubmed-32294442011-12-12 Nonlinear changes in the activity of the oxygen-dependent demethylase system in Rhodococcus erythropolis cells in the presence of low and very low doses of formaldehyde Malarczyk, Elżbieta Pazdzioch-Czochra, Marzanna Grąz, Marcin Kochmańska-Rdest, Janina Jarosz-Wilkołazka, Anna Nonlinear Biomed Phys Research The effect of exogenous, highly diluted formaldehyde on the rate of demethylation/re-methylation of veratric acid by the bacteria Rhodococcus erythropolis was studied using electrophoretic and microscopic techniques. The activity of 4-O-demethylase, responsible for accumulation of vanillic acid, and the levels of veratric and vanillic acids were determined using capillary electrophoresis. Formaldehyde was serially diluted at 1:100 ratios, and the total number of iterations was 20. After incubation of the successive dilutions of formaldehyde with the bacteria, demethylase activity oscillated in a sinusoidal manner. It was established using capillary electrophoresis that methylation of vanillic acid to veratric acid occurred at a double rate, as shown by the doubled fluctuation in the concentration of veratrate. There were also changes in the NADH oxidase activity, which is associated with methylation processes. Microscopic observations revealed the presence of numerous enlarged vacuoles in bacterial cells during the accumulation of large amounts of vanillic acid, and their disappearance together with a decrease in 4-O-demethylase activity. The presented results give evidence for the ability of living cells to detect the presence of submolecular concentrations of biological effectors in their environment and provide a basis for a scientific explanation of the law of hormesis and the therapeutic effect of homeopathic dilutions. BioMed Central 2011-11-21 /pmc/articles/PMC3229444/ /pubmed/22104369 http://dx.doi.org/10.1186/1753-4631-5-9 Text en Copyright ©2011 Malarczyk et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Malarczyk, Elżbieta
Pazdzioch-Czochra, Marzanna
Grąz, Marcin
Kochmańska-Rdest, Janina
Jarosz-Wilkołazka, Anna
Nonlinear changes in the activity of the oxygen-dependent demethylase system in Rhodococcus erythropolis cells in the presence of low and very low doses of formaldehyde
title Nonlinear changes in the activity of the oxygen-dependent demethylase system in Rhodococcus erythropolis cells in the presence of low and very low doses of formaldehyde
title_full Nonlinear changes in the activity of the oxygen-dependent demethylase system in Rhodococcus erythropolis cells in the presence of low and very low doses of formaldehyde
title_fullStr Nonlinear changes in the activity of the oxygen-dependent demethylase system in Rhodococcus erythropolis cells in the presence of low and very low doses of formaldehyde
title_full_unstemmed Nonlinear changes in the activity of the oxygen-dependent demethylase system in Rhodococcus erythropolis cells in the presence of low and very low doses of formaldehyde
title_short Nonlinear changes in the activity of the oxygen-dependent demethylase system in Rhodococcus erythropolis cells in the presence of low and very low doses of formaldehyde
title_sort nonlinear changes in the activity of the oxygen-dependent demethylase system in rhodococcus erythropolis cells in the presence of low and very low doses of formaldehyde
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229444/
https://www.ncbi.nlm.nih.gov/pubmed/22104369
http://dx.doi.org/10.1186/1753-4631-5-9
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