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DNA Electrophoretic Migration Patterns Change after Exposure of Jurkat Cells to a Single Intense Nanosecond Electric Pulse

Intense nanosecond pulsed electric fields (nsPEFs) interact with cellular membranes and intracellular structures. Investigating how cells respond to nanosecond pulses is essential for a) development of biomedical applications of nsPEFs, including cancer therapy, and b) better understanding of the me...

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Autores principales: Romeo, Stefania, Zeni, Luigi, Sarti, Maurizio, Sannino, Anna, Scarfì, Maria Rosaria, Vernier, P. Thomas, Zeni, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229573/
https://www.ncbi.nlm.nih.gov/pubmed/22164287
http://dx.doi.org/10.1371/journal.pone.0028419
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author Romeo, Stefania
Zeni, Luigi
Sarti, Maurizio
Sannino, Anna
Scarfì, Maria Rosaria
Vernier, P. Thomas
Zeni, Olga
author_facet Romeo, Stefania
Zeni, Luigi
Sarti, Maurizio
Sannino, Anna
Scarfì, Maria Rosaria
Vernier, P. Thomas
Zeni, Olga
author_sort Romeo, Stefania
collection PubMed
description Intense nanosecond pulsed electric fields (nsPEFs) interact with cellular membranes and intracellular structures. Investigating how cells respond to nanosecond pulses is essential for a) development of biomedical applications of nsPEFs, including cancer therapy, and b) better understanding of the mechanisms underlying such bioelectrical effects. In this work, we explored relatively mild exposure conditions to provide insight into weak, reversible effects, laying a foundation for a better understanding of the interaction mechanisms and kinetics underlying nsPEF bio-effects. In particular, we report changes in the nucleus of Jurkat cells (human lymphoblastoid T cells) exposed to single pulses of 60 ns duration and 1.0, 1.5 and 2.5 MV/m amplitudes, which do not affect cell growth and viability. A dose-dependent reduction in alkaline comet-assayed DNA migration is observed immediately after nsPEF exposure, accompanied by permeabilization of the plasma membrane (YO-PRO-1 uptake). Comet assay profiles return to normal within 60 minutes after pulse delivery at the highest pulse amplitude tested, indicating that our exposure protocol affects the nucleus, modifying DNA electrophoretic migration patterns.
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spelling pubmed-32295732011-12-07 DNA Electrophoretic Migration Patterns Change after Exposure of Jurkat Cells to a Single Intense Nanosecond Electric Pulse Romeo, Stefania Zeni, Luigi Sarti, Maurizio Sannino, Anna Scarfì, Maria Rosaria Vernier, P. Thomas Zeni, Olga PLoS One Research Article Intense nanosecond pulsed electric fields (nsPEFs) interact with cellular membranes and intracellular structures. Investigating how cells respond to nanosecond pulses is essential for a) development of biomedical applications of nsPEFs, including cancer therapy, and b) better understanding of the mechanisms underlying such bioelectrical effects. In this work, we explored relatively mild exposure conditions to provide insight into weak, reversible effects, laying a foundation for a better understanding of the interaction mechanisms and kinetics underlying nsPEF bio-effects. In particular, we report changes in the nucleus of Jurkat cells (human lymphoblastoid T cells) exposed to single pulses of 60 ns duration and 1.0, 1.5 and 2.5 MV/m amplitudes, which do not affect cell growth and viability. A dose-dependent reduction in alkaline comet-assayed DNA migration is observed immediately after nsPEF exposure, accompanied by permeabilization of the plasma membrane (YO-PRO-1 uptake). Comet assay profiles return to normal within 60 minutes after pulse delivery at the highest pulse amplitude tested, indicating that our exposure protocol affects the nucleus, modifying DNA electrophoretic migration patterns. Public Library of Science 2011-12-02 /pmc/articles/PMC3229573/ /pubmed/22164287 http://dx.doi.org/10.1371/journal.pone.0028419 Text en Romeo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Romeo, Stefania
Zeni, Luigi
Sarti, Maurizio
Sannino, Anna
Scarfì, Maria Rosaria
Vernier, P. Thomas
Zeni, Olga
DNA Electrophoretic Migration Patterns Change after Exposure of Jurkat Cells to a Single Intense Nanosecond Electric Pulse
title DNA Electrophoretic Migration Patterns Change after Exposure of Jurkat Cells to a Single Intense Nanosecond Electric Pulse
title_full DNA Electrophoretic Migration Patterns Change after Exposure of Jurkat Cells to a Single Intense Nanosecond Electric Pulse
title_fullStr DNA Electrophoretic Migration Patterns Change after Exposure of Jurkat Cells to a Single Intense Nanosecond Electric Pulse
title_full_unstemmed DNA Electrophoretic Migration Patterns Change after Exposure of Jurkat Cells to a Single Intense Nanosecond Electric Pulse
title_short DNA Electrophoretic Migration Patterns Change after Exposure of Jurkat Cells to a Single Intense Nanosecond Electric Pulse
title_sort dna electrophoretic migration patterns change after exposure of jurkat cells to a single intense nanosecond electric pulse
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229573/
https://www.ncbi.nlm.nih.gov/pubmed/22164287
http://dx.doi.org/10.1371/journal.pone.0028419
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