Cargando…
Structural Analysis of Fungal Cerebrosides
Of the ceramide monohexosides (CMHs), gluco- and galactosyl-ceramides are the main neutral glycosphingolipids expressed in fungal cells. Their structural determination is greatly dependent on the use of mass spectrometric techniques, including fast atom bombardment-mass spectrometry, electrospray io...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Research Foundation
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230030/ https://www.ncbi.nlm.nih.gov/pubmed/22164155 http://dx.doi.org/10.3389/fmicb.2011.00239 |
_version_ | 1782218026280550400 |
---|---|
author | Barreto-Bergter, Eliana Sassaki, Guilherme L. de Souza, Lauro M. |
author_facet | Barreto-Bergter, Eliana Sassaki, Guilherme L. de Souza, Lauro M. |
author_sort | Barreto-Bergter, Eliana |
collection | PubMed |
description | Of the ceramide monohexosides (CMHs), gluco- and galactosyl-ceramides are the main neutral glycosphingolipids expressed in fungal cells. Their structural determination is greatly dependent on the use of mass spectrometric techniques, including fast atom bombardment-mass spectrometry, electrospray ionization, and energy collision-induced dissociation mass spectrometry. Nuclear magnetic resonance has also been used successfully. Such a combination of techniques, combined with classical analytical separation, such as high-performance thin layer chromatography and column chromatography, has led to the structural elucidation of a great number of fungal CMHs. The structure of fungal CMH is conserved among fungal species and consists of a glucose or galactose residue attached to a ceramide moiety containing 9-methyl-4,8-sphingadienine with an amidic linkage to hydroxylated fatty acids, most commonly having 16 or 18 carbon atoms and unsaturation between C-3 and C-4. Along with their unique structural characteristics, fungal CMHs have a peculiar subcellular distribution and striking biological properties. Fungal cerebrosides were also characterized as antigenic molecules directly or indirectly involved in cell growth or differentiation in Schizophyllum commune, Cryptococcus neoformans, Pseudallescheria boydii, Candida albicans, Aspergillus nidulans, Aspergillus fumigatus, and Colletotrichum gloeosporioides. Besides classical techniques for cerebroside (CMH) analysis, we now describe new approaches, combining conventional thin layer chromatography and mass spectrometry, as well as emerging technologies for subcellular localization and distribution of glycosphingolipids by secondary ion mass spectrometry and imaging matrix-assisted laser desorption ionization time-of-flight. |
format | Online Article Text |
id | pubmed-3230030 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Frontiers Research Foundation |
record_format | MEDLINE/PubMed |
spelling | pubmed-32300302011-12-07 Structural Analysis of Fungal Cerebrosides Barreto-Bergter, Eliana Sassaki, Guilherme L. de Souza, Lauro M. Front Microbiol Microbiology Of the ceramide monohexosides (CMHs), gluco- and galactosyl-ceramides are the main neutral glycosphingolipids expressed in fungal cells. Their structural determination is greatly dependent on the use of mass spectrometric techniques, including fast atom bombardment-mass spectrometry, electrospray ionization, and energy collision-induced dissociation mass spectrometry. Nuclear magnetic resonance has also been used successfully. Such a combination of techniques, combined with classical analytical separation, such as high-performance thin layer chromatography and column chromatography, has led to the structural elucidation of a great number of fungal CMHs. The structure of fungal CMH is conserved among fungal species and consists of a glucose or galactose residue attached to a ceramide moiety containing 9-methyl-4,8-sphingadienine with an amidic linkage to hydroxylated fatty acids, most commonly having 16 or 18 carbon atoms and unsaturation between C-3 and C-4. Along with their unique structural characteristics, fungal CMHs have a peculiar subcellular distribution and striking biological properties. Fungal cerebrosides were also characterized as antigenic molecules directly or indirectly involved in cell growth or differentiation in Schizophyllum commune, Cryptococcus neoformans, Pseudallescheria boydii, Candida albicans, Aspergillus nidulans, Aspergillus fumigatus, and Colletotrichum gloeosporioides. Besides classical techniques for cerebroside (CMH) analysis, we now describe new approaches, combining conventional thin layer chromatography and mass spectrometry, as well as emerging technologies for subcellular localization and distribution of glycosphingolipids by secondary ion mass spectrometry and imaging matrix-assisted laser desorption ionization time-of-flight. Frontiers Research Foundation 2011-12-05 /pmc/articles/PMC3230030/ /pubmed/22164155 http://dx.doi.org/10.3389/fmicb.2011.00239 Text en Copyright © 2011 Barreto-Bergter, Sassaki and de Souza. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited. |
spellingShingle | Microbiology Barreto-Bergter, Eliana Sassaki, Guilherme L. de Souza, Lauro M. Structural Analysis of Fungal Cerebrosides |
title | Structural Analysis of Fungal Cerebrosides |
title_full | Structural Analysis of Fungal Cerebrosides |
title_fullStr | Structural Analysis of Fungal Cerebrosides |
title_full_unstemmed | Structural Analysis of Fungal Cerebrosides |
title_short | Structural Analysis of Fungal Cerebrosides |
title_sort | structural analysis of fungal cerebrosides |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230030/ https://www.ncbi.nlm.nih.gov/pubmed/22164155 http://dx.doi.org/10.3389/fmicb.2011.00239 |
work_keys_str_mv | AT barretobergtereliana structuralanalysisoffungalcerebrosides AT sassakiguilhermel structuralanalysisoffungalcerebrosides AT desouzalaurom structuralanalysisoffungalcerebrosides |