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Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity

Isothermal titration calorimetry (ITC) is a useful tool for understanding the complete thermodynamic picture of a binding reaction. In biological sciences, macromolecular interactions are essential in understanding the machinery of the cell. Experimental conditions, such as buffer and temperature, c...

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Detalles Bibliográficos
Autores principales: Duff,, Michael R., Grubbs, Jordan, Howell, Elizabeth E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230191/
https://www.ncbi.nlm.nih.gov/pubmed/21931288
http://dx.doi.org/10.3791/2796