Cargando…

Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity

Isothermal titration calorimetry (ITC) is a useful tool for understanding the complete thermodynamic picture of a binding reaction. In biological sciences, macromolecular interactions are essential in understanding the machinery of the cell. Experimental conditions, such as buffer and temperature, c...

Descripción completa

Detalles Bibliográficos
Autores principales:	Duff,, Michael R., Grubbs, Jordan, Howell, Elizabeth E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2011
Materias:	Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230191/ https://www.ncbi.nlm.nih.gov/pubmed/21931288 http://dx.doi.org/10.3791/2796

Ejemplares similares

Enzyme Kinetics by Isothermal Titration Calorimetry: Allostery, Inhibition, and Dynamics
por: Wang, Yun, et al.
Publicado: (2020)

Titration Calorimetry Standards and the Precision of Isothermal Titration Calorimetry Data
por: Baranauskienė, Lina, et al.
Publicado: (2009)

Reconciling isothermal titration calorimetry analyses of interactions between complexin and truncated SNARE complexes
por: Prinslow, Eric A, et al.
Publicado: (2017)

Analysis of Cooperativity by Isothermal Titration Calorimetry
por: Brown, Alan
Publicado: (2009)

Rapid measurement of inhibitor binding kinetics by isothermal titration calorimetry
por: Di Trani, Justin M., et al.
Publicado: (2018)

On the Physical Meaning of the Isothermal Titration Calorimetry Measurements in Calorimeters with Full Cells
por: Grolier, Jean-Pierre E., et al.
Publicado: (2009)

Bayesian analysis of isothermal titration calorimetry for binding thermodynamics
por: Nguyen, Trung Hai, et al.
Publicado: (2018)

Isothermal titration calorimetry of ion-coupled membrane transporters
por: Boudker, Olga, et al.
Publicado: (2015)

Applications of Isothermal Titration Calorimetry in Biophysical Studies of G-quadruplexes
por: Pagano, Bruno, et al.
Publicado: (2009)

Applications of isothermal titration calorimetry in protein folding and molecular recognition
por: Liang, Y.
Publicado: (2006)

Estimation of non-constant variance in isothermal titration calorimetry using an ITC measurement model
por: Ge, Xiujie, et al.
Publicado: (2020)

Real-Time Monitoring of Membrane-Protein Reconstitution by Isothermal Titration Calorimetry
por: Jahnke, Nadin, et al.
Publicado: (2013)

Bayesian regression and model selection for isothermal titration calorimetry with enantiomeric mixtures
por: Nguyen, Trung Hai, et al.
Publicado: (2022)

Isothermal titration calorimetry and surface plasmon resonance analysis using the dynamic approach
por: Krishnamoorthy, Ganesh Kumar, et al.
Publicado: (2019)

Tannins Can Have Direct Interactions with Anthelmintics: Investigations by Isothermal Titration Calorimetry
por: Sillanpää, Mimosa, et al.
Publicado: (2023)

Multi-site binding of epigallocatechin gallate to human serum albumin measured by NMR and isothermal titration calorimetry
por: Eaton, Joshua D., et al.
Publicado: (2017)

Isothermal Titration Calorimetry Directly Measures the Selective Swelling of Block Copolymer Vesicles in the Presence of Organic Acid
por: Zhang, Qiuya, et al.
Publicado: (2022)

Hydrophobic interaction chromatography of proteins: Studies of unfolding upon adsorption by isothermal titration calorimetry
por: Rodler, Agnes, et al.
Publicado: (2018)

Isothermal titration calorimetry with micelles: Thermodynamics of inhibitor binding to carnitine palmitoyltransferase 2 membrane protein()
por: Perspicace, Samantha, et al.
Publicado: (2013)

Thermodynamics of clay–drug complex dispersions: Isothermal titration calorimetry and high-performance liquid chromatography
por: Totea, Ana-Maria, et al.
Publicado: (2020)

Thermal Activity in Affinity Separation Techniques Such as Liquid–Liquid Extraction Analyzed by Isothermal Titration Calorimetry and Accuracy Analysis of the Technique in the Molar Concentration Domain
por: Sprakel, Lisette M. J., et al.
Publicado: (2018)

Label-free protocol to quantify protein affinity using isothermal titration calorimetry and bio-layer interferometry of a human eIF5-mimic protein
por: Singh, Chingakham Ranjit, et al.
Publicado: (2022)

Isothermal titration calorimetry uncovers substrate promiscuity of bicupin oxalate oxidase from Ceriporiopsis subvermispora
por: Rana, Hassan, et al.
Publicado: (2016)

Thermodynamics of Interactions Between Charged Surfactants and Ionic Poly(amino acids) by Isothermal Titration Calorimetry
por: Skvarnavičius, Gediminas, et al.
Publicado: (2019)

Interactions between Hydrolysable Tannins and Lipid Vesicles from Escherichia coli with Isothermal Titration Calorimetry
por: Virtanen, Valtteri, et al.
Publicado: (2022)

Application of In Silico Filtering and Isothermal Titration Calorimetry for the Discovery of Small Molecule Inhibitors of MDM2
por: Alali, Hen, et al.
Publicado: (2022)

The use of isothermal titration calorimetry for the assay of enzyme activity: Application in higher education practical classes
por: Siddiqui, Khawar S., et al.
Publicado: (2022)

Characterization of the kinetic and thermodynamic landscape of RNA folding using a novel application of isothermal titration calorimetry
por: Vander Meulen, Kirk A., et al.
Publicado: (2012)

Isothermal Titration Calorimetry Enables Rapid Characterization of Enzyme Kinetics and Inhibition for the Human Soluble Epoxide Hydrolase
por: Abis, Giancarlo, et al.
Publicado: (2019)

Cyclodextrin Complex Formation with Water-Soluble Drugs: Conclusions from Isothermal Titration Calorimetry and Molecular Modeling
por: Shalaby, Karim S., et al.
Publicado: (2021)

Titration calorimetry: from concept to application
por: Hansen, Lee D, et al.
Publicado: (2018)

The mechanism of interactions between tea polyphenols and porcine pancreatic alpha‐amylase: Analysis by inhibition kinetics, fluorescence quenching, differential scanning calorimetry and isothermal titration calorimetry
por: Sun, Lijun, et al.
Publicado: (2017)

Measuring the sequence-affinity landscape of antibodies with massively parallel titration curves
por: Adams, Rhys M, et al.
Publicado: (2016)

Stealth Nanoparticles Grafted with Dense Polymer Brushes Display Adsorption of Serum Protein Investigated by Isothermal Titration Calorimetry
por: Gal, Noga, et al.
Publicado: (2018)

Ligand effects on phase separation of multivalent macromolecules
por: Ruff, Kiersten M., et al.
Publicado: (2021)

Editorial: Analytical devices based on immobilized macromolecules for structural and activity/affinity studies in drug discovery
por: Temporini, Caterina, et al.
Publicado: (2022)

Determination of thermodynamic parameters of Xerocomus chrysenteron lectin interactions with N-acetylgalactosamine and Thomsen-Friedenreich antigen by isothermal titration calorimetry
por: Damian, Luminita, et al.
Publicado: (2005)

Native ESI Mass Spectrometry Can Help to Avoid Wrong Interpretations from Isothermal Titration Calorimetry in Difficult Situations
por: Wolff, Philippe, et al.
Publicado: (2016)

A Source of Systematic Errors in the Determination of Critical Micelle Concentration and Micellization Enthalpy by Graphical Methods in Isothermal Titration Calorimetry
por: Corea, Mónica, et al.
Publicado: (2021)

Bayesian Regression Quantifies Uncertainty of Binding Parameters from Isothermal Titration Calorimetry More Accurately Than Error Propagation
por: La, Van N. T., et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_un4qork2vv9ngm0mc6ir6tk89s