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Maternal and Gestational Factors and Micronucleus Frequencies in Umbilical Blood: The NewGeneris Rhea Cohort in Crete

Background: The use of cancer-related biomarkers in newborns has been very limited. Objective: We investigated the formation of micronuclei (MN) in full-term and preterm newborns and their mothers from the Rhea cohort (Crete), applying for the first time in cord blood a validated semiautomated analy...

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Autores principales: Vande Loock, Kim, Fthenou, Eleni, Decordier, Ilse, Chalkiadaki, Georgia, Keramarou, Maria, Plas, Gina, Roelants, Mathieu, Kleinjans, Jos, Chatzi, Leda, Merlo, Franco, Kogevinas, Manolis, Kirsch-Volders, Micheline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Institute of Environmental Health Sciences 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230441/
https://www.ncbi.nlm.nih.gov/pubmed/21622084
http://dx.doi.org/10.1289/ehp.1003246
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author Vande Loock, Kim
Fthenou, Eleni
Decordier, Ilse
Chalkiadaki, Georgia
Keramarou, Maria
Plas, Gina
Roelants, Mathieu
Kleinjans, Jos
Chatzi, Leda
Merlo, Franco
Kogevinas, Manolis
Kirsch-Volders, Micheline
author_facet Vande Loock, Kim
Fthenou, Eleni
Decordier, Ilse
Chalkiadaki, Georgia
Keramarou, Maria
Plas, Gina
Roelants, Mathieu
Kleinjans, Jos
Chatzi, Leda
Merlo, Franco
Kogevinas, Manolis
Kirsch-Volders, Micheline
author_sort Vande Loock, Kim
collection PubMed
description Background: The use of cancer-related biomarkers in newborns has been very limited. Objective: We investigated the formation of micronuclei (MN) in full-term and preterm newborns and their mothers from the Rhea cohort (Crete), applying for the first time in cord blood a validated semiautomated analysis system, in both mono- and binucleated T lymphocytes. Methods: We assessed MN frequencies in peripheral blood samples from the mothers and in umbilical cord blood samples. We calculated MN in mononucleated (MNMONO) and binucleated (MNBN) T lymphocytes and the cytokinesis block proliferation index (CBPI) in 251 newborns (224 full term) and 223 mothers, including 182 mother–child pairs. Demographic and lifestyle characteristics were collected. Results: We observed significantly higher MNBN and CBPI levels in mothers than in newborns. In newborns, MNMONO and MNBN were correlated (r = 0.35, p < 0.001), and we found a moderate correlation between MNMONO in mothers and newborns (r = 0.26, p < 0.001). MNMONO frequencies in newborns were positively associated with the mother’s body mass index and inversely associated with gestational age and mother’s age, but we found no significant predictors of MNBN or CBPI in newborns. Conclusions: Although confirmation is needed by a larger study population, the results indicate the importance of taking into account both mono- and binucleated T lymphocytes for biomonitoring of newborns, because the first reflects damage expressed during in vivo cell division and accumulated in utero, and the latter includes additional damage expressed as MN during the in vitro culture step.
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spelling pubmed-32304412011-12-15 Maternal and Gestational Factors and Micronucleus Frequencies in Umbilical Blood: The NewGeneris Rhea Cohort in Crete Vande Loock, Kim Fthenou, Eleni Decordier, Ilse Chalkiadaki, Georgia Keramarou, Maria Plas, Gina Roelants, Mathieu Kleinjans, Jos Chatzi, Leda Merlo, Franco Kogevinas, Manolis Kirsch-Volders, Micheline Environ Health Perspect Research Background: The use of cancer-related biomarkers in newborns has been very limited. Objective: We investigated the formation of micronuclei (MN) in full-term and preterm newborns and their mothers from the Rhea cohort (Crete), applying for the first time in cord blood a validated semiautomated analysis system, in both mono- and binucleated T lymphocytes. Methods: We assessed MN frequencies in peripheral blood samples from the mothers and in umbilical cord blood samples. We calculated MN in mononucleated (MNMONO) and binucleated (MNBN) T lymphocytes and the cytokinesis block proliferation index (CBPI) in 251 newborns (224 full term) and 223 mothers, including 182 mother–child pairs. Demographic and lifestyle characteristics were collected. Results: We observed significantly higher MNBN and CBPI levels in mothers than in newborns. In newborns, MNMONO and MNBN were correlated (r = 0.35, p < 0.001), and we found a moderate correlation between MNMONO in mothers and newborns (r = 0.26, p < 0.001). MNMONO frequencies in newborns were positively associated with the mother’s body mass index and inversely associated with gestational age and mother’s age, but we found no significant predictors of MNBN or CBPI in newborns. Conclusions: Although confirmation is needed by a larger study population, the results indicate the importance of taking into account both mono- and binucleated T lymphocytes for biomonitoring of newborns, because the first reflects damage expressed during in vivo cell division and accumulated in utero, and the latter includes additional damage expressed as MN during the in vitro culture step. National Institute of Environmental Health Sciences 2011-05-27 2011-10 /pmc/articles/PMC3230441/ /pubmed/21622084 http://dx.doi.org/10.1289/ehp.1003246 Text en http://creativecommons.org/publicdomain/mark/1.0/ Publication of EHP lies in the public domain and is therefore without copyright. All text from EHP may be reprinted freely. Use of materials published in EHP should be acknowledged (for example, ?Reproduced with permission from Environmental Health Perspectives?); pertinent reference information should be provided for the article from which the material was reproduced. Articles from EHP, especially the News section, may contain photographs or illustrations copyrighted by other commercial organizations or individuals that may not be used without obtaining prior approval from the holder of the copyright.
spellingShingle Research
Vande Loock, Kim
Fthenou, Eleni
Decordier, Ilse
Chalkiadaki, Georgia
Keramarou, Maria
Plas, Gina
Roelants, Mathieu
Kleinjans, Jos
Chatzi, Leda
Merlo, Franco
Kogevinas, Manolis
Kirsch-Volders, Micheline
Maternal and Gestational Factors and Micronucleus Frequencies in Umbilical Blood: The NewGeneris Rhea Cohort in Crete
title Maternal and Gestational Factors and Micronucleus Frequencies in Umbilical Blood: The NewGeneris Rhea Cohort in Crete
title_full Maternal and Gestational Factors and Micronucleus Frequencies in Umbilical Blood: The NewGeneris Rhea Cohort in Crete
title_fullStr Maternal and Gestational Factors and Micronucleus Frequencies in Umbilical Blood: The NewGeneris Rhea Cohort in Crete
title_full_unstemmed Maternal and Gestational Factors and Micronucleus Frequencies in Umbilical Blood: The NewGeneris Rhea Cohort in Crete
title_short Maternal and Gestational Factors and Micronucleus Frequencies in Umbilical Blood: The NewGeneris Rhea Cohort in Crete
title_sort maternal and gestational factors and micronucleus frequencies in umbilical blood: the newgeneris rhea cohort in crete
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230441/
https://www.ncbi.nlm.nih.gov/pubmed/21622084
http://dx.doi.org/10.1289/ehp.1003246
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