RNA targets of wild-type and mutant FET family proteins

FUS, EWSR1 and TAF15, constituting the FET protein family, are abundant, highly conserved RNA-binding proteins with important roles in oncogenesis and neuronal disease, yet their RNA targets and recognition element are unknown. Using PAR-CLIP, we defined global RNA targets of all human FET proteins...

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Autores principales: Hoell, Jessica I., Larsson, Erik, Runge, Simon, Nusbaum, Jeffrey D., Duggimpudi, Sujitha, Farazi, Thalia A., Hafner, Markus, Borkhardt, Arndt, Sander, Chris, Tuschl, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230689/
https://www.ncbi.nlm.nih.gov/pubmed/22081015
http://dx.doi.org/10.1038/nsmb.2163
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author Hoell, Jessica I.
Larsson, Erik
Runge, Simon
Nusbaum, Jeffrey D.
Duggimpudi, Sujitha
Farazi, Thalia A.
Hafner, Markus
Borkhardt, Arndt
Sander, Chris
Tuschl, Thomas
author_facet Hoell, Jessica I.
Larsson, Erik
Runge, Simon
Nusbaum, Jeffrey D.
Duggimpudi, Sujitha
Farazi, Thalia A.
Hafner, Markus
Borkhardt, Arndt
Sander, Chris
Tuschl, Thomas
author_sort Hoell, Jessica I.
collection PubMed
description FUS, EWSR1 and TAF15, constituting the FET protein family, are abundant, highly conserved RNA-binding proteins with important roles in oncogenesis and neuronal disease, yet their RNA targets and recognition element are unknown. Using PAR-CLIP, we defined global RNA targets of all human FET proteins and two ALS-causing human FUS mutants. FET members displayed similar binding profiles while mutant FUS showed a drastically altered binding pattern, consistent with changes in subcellular localization.
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spelling pubmed-32306892012-06-01 RNA targets of wild-type and mutant FET family proteins Hoell, Jessica I. Larsson, Erik Runge, Simon Nusbaum, Jeffrey D. Duggimpudi, Sujitha Farazi, Thalia A. Hafner, Markus Borkhardt, Arndt Sander, Chris Tuschl, Thomas Nat Struct Mol Biol Article FUS, EWSR1 and TAF15, constituting the FET protein family, are abundant, highly conserved RNA-binding proteins with important roles in oncogenesis and neuronal disease, yet their RNA targets and recognition element are unknown. Using PAR-CLIP, we defined global RNA targets of all human FET proteins and two ALS-causing human FUS mutants. FET members displayed similar binding profiles while mutant FUS showed a drastically altered binding pattern, consistent with changes in subcellular localization. 2011-11-13 /pmc/articles/PMC3230689/ /pubmed/22081015 http://dx.doi.org/10.1038/nsmb.2163 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Hoell, Jessica I.
Larsson, Erik
Runge, Simon
Nusbaum, Jeffrey D.
Duggimpudi, Sujitha
Farazi, Thalia A.
Hafner, Markus
Borkhardt, Arndt
Sander, Chris
Tuschl, Thomas
RNA targets of wild-type and mutant FET family proteins
title RNA targets of wild-type and mutant FET family proteins
title_full RNA targets of wild-type and mutant FET family proteins
title_fullStr RNA targets of wild-type and mutant FET family proteins
title_full_unstemmed RNA targets of wild-type and mutant FET family proteins
title_short RNA targets of wild-type and mutant FET family proteins
title_sort rna targets of wild-type and mutant fet family proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230689/
https://www.ncbi.nlm.nih.gov/pubmed/22081015
http://dx.doi.org/10.1038/nsmb.2163
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