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Development of an Electrochemical Biosensor for the Detection of Aflatoxin M(1) in Milk
We have developed an electrochemical immunosensor for the detection of ultratrace amounts of aflatoxin M(1) (AFM(1)) in food products. The sensor was based on a competitive immunoassay using horseradish peroxidase (HRP) as a tag. Magnetic nanoparticles coated with antibody (anti-AFM(1)) were used to...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Molecular Diversity Preservation International (MDPI)
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230981/ https://www.ncbi.nlm.nih.gov/pubmed/22163418 http://dx.doi.org/10.3390/s101009439 |
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author | Paniel, Nathalie Radoi, Antonio Marty, Jean-Louis |
author_facet | Paniel, Nathalie Radoi, Antonio Marty, Jean-Louis |
author_sort | Paniel, Nathalie |
collection | PubMed |
description | We have developed an electrochemical immunosensor for the detection of ultratrace amounts of aflatoxin M(1) (AFM(1)) in food products. The sensor was based on a competitive immunoassay using horseradish peroxidase (HRP) as a tag. Magnetic nanoparticles coated with antibody (anti-AFM(1)) were used to separate the bound and unbound fractions. The samples containing AFM(1) were incubated with a fixed amount of antibody and tracer [AFM(1) linked to HRP (conjugate)] until the system reached equilibrium. Competition occurs between the antigen (AFM(1)) and the conjugate for the antibody. Then, the mixture was deposited on the surface of screen-printed carbon electrodes, and the mediator [5-methylphenazinium methyl sulphate (MPMS)] was added. The enzymatic response was measured amperometrically. A standard range (0, 0.005, 0.01, 0.025, 0.05, 0.1, 0.25, 0.3, 0.4 and 0.5 ppb) of AFM(1)-contaminated milk from the ELISA kit was used to obtain a standard curve for AFM(1). To test the detection sensitivity of our sensor, samples of commercial milk were supplemented at 0.01, 0.025, 0.05 or 0.1 ppb with AFM(1). Our immunosensor has a low detection limit (0.01 ppb), which is under the recommended level of AFM(1) [0.05 μg L-1 (ppb)], and has good reproducibility. |
format | Online Article Text |
id | pubmed-3230981 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-32309812011-12-07 Development of an Electrochemical Biosensor for the Detection of Aflatoxin M(1) in Milk Paniel, Nathalie Radoi, Antonio Marty, Jean-Louis Sensors (Basel) Article We have developed an electrochemical immunosensor for the detection of ultratrace amounts of aflatoxin M(1) (AFM(1)) in food products. The sensor was based on a competitive immunoassay using horseradish peroxidase (HRP) as a tag. Magnetic nanoparticles coated with antibody (anti-AFM(1)) were used to separate the bound and unbound fractions. The samples containing AFM(1) were incubated with a fixed amount of antibody and tracer [AFM(1) linked to HRP (conjugate)] until the system reached equilibrium. Competition occurs between the antigen (AFM(1)) and the conjugate for the antibody. Then, the mixture was deposited on the surface of screen-printed carbon electrodes, and the mediator [5-methylphenazinium methyl sulphate (MPMS)] was added. The enzymatic response was measured amperometrically. A standard range (0, 0.005, 0.01, 0.025, 0.05, 0.1, 0.25, 0.3, 0.4 and 0.5 ppb) of AFM(1)-contaminated milk from the ELISA kit was used to obtain a standard curve for AFM(1). To test the detection sensitivity of our sensor, samples of commercial milk were supplemented at 0.01, 0.025, 0.05 or 0.1 ppb with AFM(1). Our immunosensor has a low detection limit (0.01 ppb), which is under the recommended level of AFM(1) [0.05 μg L-1 (ppb)], and has good reproducibility. Molecular Diversity Preservation International (MDPI) 2010-10-20 /pmc/articles/PMC3230981/ /pubmed/22163418 http://dx.doi.org/10.3390/s101009439 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Paniel, Nathalie Radoi, Antonio Marty, Jean-Louis Development of an Electrochemical Biosensor for the Detection of Aflatoxin M(1) in Milk |
title | Development of an Electrochemical Biosensor for the Detection of Aflatoxin M(1) in Milk |
title_full | Development of an Electrochemical Biosensor for the Detection of Aflatoxin M(1) in Milk |
title_fullStr | Development of an Electrochemical Biosensor for the Detection of Aflatoxin M(1) in Milk |
title_full_unstemmed | Development of an Electrochemical Biosensor for the Detection of Aflatoxin M(1) in Milk |
title_short | Development of an Electrochemical Biosensor for the Detection of Aflatoxin M(1) in Milk |
title_sort | development of an electrochemical biosensor for the detection of aflatoxin m(1) in milk |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230981/ https://www.ncbi.nlm.nih.gov/pubmed/22163418 http://dx.doi.org/10.3390/s101009439 |
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