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Cytochrome C Biosensor—A Model for Gas Sensing
This work is about gas biosensing with a cytochrome c biosensor. Emphasis is put on the analysis of the sensing process and a mathematical model to make predictions about the biosensor response. Reliable predictions about biosensor responses can provide valuable information and facilitate biosensor...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3231445/ https://www.ncbi.nlm.nih.gov/pubmed/22163937 http://dx.doi.org/10.3390/s110605968 |
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author | Hulko, Michael Hospach, Ingeborg Krasteva, Nadejda Nelles, Gabriele |
author_facet | Hulko, Michael Hospach, Ingeborg Krasteva, Nadejda Nelles, Gabriele |
author_sort | Hulko, Michael |
collection | PubMed |
description | This work is about gas biosensing with a cytochrome c biosensor. Emphasis is put on the analysis of the sensing process and a mathematical model to make predictions about the biosensor response. Reliable predictions about biosensor responses can provide valuable information and facilitate biosensor development, particularly at an early development stage. The sensing process comprises several individual steps, such as phase partition equilibrium, intermediate reactions, mass-transport, and reaction kinetics, which take place in and between the gas and liquid phases. A quantitative description of each step was worked out and finally combined into a mathematical model. The applicability of the model was demonstrated for a particular example of methanethiol gas detection by a cytochrome c biosensor. The model allowed us to predict the optical readout response of the biosensor from tabulated data and data obtained in simple liquid phase experiments. The prediction was experimentally verified with a planar three-electrode electro-optical cytochrome c biosensor in contact with methanethiol gas in a gas tight spectroelectrochemical measurement cell. |
format | Online Article Text |
id | pubmed-3231445 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-32314452011-12-07 Cytochrome C Biosensor—A Model for Gas Sensing Hulko, Michael Hospach, Ingeborg Krasteva, Nadejda Nelles, Gabriele Sensors (Basel) Article This work is about gas biosensing with a cytochrome c biosensor. Emphasis is put on the analysis of the sensing process and a mathematical model to make predictions about the biosensor response. Reliable predictions about biosensor responses can provide valuable information and facilitate biosensor development, particularly at an early development stage. The sensing process comprises several individual steps, such as phase partition equilibrium, intermediate reactions, mass-transport, and reaction kinetics, which take place in and between the gas and liquid phases. A quantitative description of each step was worked out and finally combined into a mathematical model. The applicability of the model was demonstrated for a particular example of methanethiol gas detection by a cytochrome c biosensor. The model allowed us to predict the optical readout response of the biosensor from tabulated data and data obtained in simple liquid phase experiments. The prediction was experimentally verified with a planar three-electrode electro-optical cytochrome c biosensor in contact with methanethiol gas in a gas tight spectroelectrochemical measurement cell. Molecular Diversity Preservation International (MDPI) 2011-06-01 /pmc/articles/PMC3231445/ /pubmed/22163937 http://dx.doi.org/10.3390/s110605968 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Hulko, Michael Hospach, Ingeborg Krasteva, Nadejda Nelles, Gabriele Cytochrome C Biosensor—A Model for Gas Sensing |
title | Cytochrome C Biosensor—A Model for Gas Sensing |
title_full | Cytochrome C Biosensor—A Model for Gas Sensing |
title_fullStr | Cytochrome C Biosensor—A Model for Gas Sensing |
title_full_unstemmed | Cytochrome C Biosensor—A Model for Gas Sensing |
title_short | Cytochrome C Biosensor—A Model for Gas Sensing |
title_sort | cytochrome c biosensor—a model for gas sensing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3231445/ https://www.ncbi.nlm.nih.gov/pubmed/22163937 http://dx.doi.org/10.3390/s110605968 |
work_keys_str_mv | AT hulkomichael cytochromecbiosensoramodelforgassensing AT hospachingeborg cytochromecbiosensoramodelforgassensing AT krastevanadejda cytochromecbiosensoramodelforgassensing AT nellesgabriele cytochromecbiosensoramodelforgassensing |