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Cytochrome C Biosensor—A Model for Gas Sensing

This work is about gas biosensing with a cytochrome c biosensor. Emphasis is put on the analysis of the sensing process and a mathematical model to make predictions about the biosensor response. Reliable predictions about biosensor responses can provide valuable information and facilitate biosensor...

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Detalles Bibliográficos
Autores principales: Hulko, Michael, Hospach, Ingeborg, Krasteva, Nadejda, Nelles, Gabriele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3231445/
https://www.ncbi.nlm.nih.gov/pubmed/22163937
http://dx.doi.org/10.3390/s110605968
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author Hulko, Michael
Hospach, Ingeborg
Krasteva, Nadejda
Nelles, Gabriele
author_facet Hulko, Michael
Hospach, Ingeborg
Krasteva, Nadejda
Nelles, Gabriele
author_sort Hulko, Michael
collection PubMed
description This work is about gas biosensing with a cytochrome c biosensor. Emphasis is put on the analysis of the sensing process and a mathematical model to make predictions about the biosensor response. Reliable predictions about biosensor responses can provide valuable information and facilitate biosensor development, particularly at an early development stage. The sensing process comprises several individual steps, such as phase partition equilibrium, intermediate reactions, mass-transport, and reaction kinetics, which take place in and between the gas and liquid phases. A quantitative description of each step was worked out and finally combined into a mathematical model. The applicability of the model was demonstrated for a particular example of methanethiol gas detection by a cytochrome c biosensor. The model allowed us to predict the optical readout response of the biosensor from tabulated data and data obtained in simple liquid phase experiments. The prediction was experimentally verified with a planar three-electrode electro-optical cytochrome c biosensor in contact with methanethiol gas in a gas tight spectroelectrochemical measurement cell.
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spelling pubmed-32314452011-12-07 Cytochrome C Biosensor—A Model for Gas Sensing Hulko, Michael Hospach, Ingeborg Krasteva, Nadejda Nelles, Gabriele Sensors (Basel) Article This work is about gas biosensing with a cytochrome c biosensor. Emphasis is put on the analysis of the sensing process and a mathematical model to make predictions about the biosensor response. Reliable predictions about biosensor responses can provide valuable information and facilitate biosensor development, particularly at an early development stage. The sensing process comprises several individual steps, such as phase partition equilibrium, intermediate reactions, mass-transport, and reaction kinetics, which take place in and between the gas and liquid phases. A quantitative description of each step was worked out and finally combined into a mathematical model. The applicability of the model was demonstrated for a particular example of methanethiol gas detection by a cytochrome c biosensor. The model allowed us to predict the optical readout response of the biosensor from tabulated data and data obtained in simple liquid phase experiments. The prediction was experimentally verified with a planar three-electrode electro-optical cytochrome c biosensor in contact with methanethiol gas in a gas tight spectroelectrochemical measurement cell. Molecular Diversity Preservation International (MDPI) 2011-06-01 /pmc/articles/PMC3231445/ /pubmed/22163937 http://dx.doi.org/10.3390/s110605968 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Hulko, Michael
Hospach, Ingeborg
Krasteva, Nadejda
Nelles, Gabriele
Cytochrome C Biosensor—A Model for Gas Sensing
title Cytochrome C Biosensor—A Model for Gas Sensing
title_full Cytochrome C Biosensor—A Model for Gas Sensing
title_fullStr Cytochrome C Biosensor—A Model for Gas Sensing
title_full_unstemmed Cytochrome C Biosensor—A Model for Gas Sensing
title_short Cytochrome C Biosensor—A Model for Gas Sensing
title_sort cytochrome c biosensor—a model for gas sensing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3231445/
https://www.ncbi.nlm.nih.gov/pubmed/22163937
http://dx.doi.org/10.3390/s110605968
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AT krastevanadejda cytochromecbiosensoramodelforgassensing
AT nellesgabriele cytochromecbiosensoramodelforgassensing