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A High-Precision Micropipette Sensor for Cellular-Level Real-Time Thermal Characterization
We report herein development of a novel glass micropipette thermal sensor fabricated in a cost-effective manner, which is capable of measuring steady thermal fluctuation at spatial resolution of ∼2 μm with an accuracy of ±0.01 °C. We produced and tested various micrometer-sized sensors, ranging from...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3231465/ https://www.ncbi.nlm.nih.gov/pubmed/22164108 http://dx.doi.org/10.3390/s110908826 |
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author | Shrestha, Ramesh Choi, Tae-Youl Chang, Wonseok Kim, Donsik |
author_facet | Shrestha, Ramesh Choi, Tae-Youl Chang, Wonseok Kim, Donsik |
author_sort | Shrestha, Ramesh |
collection | PubMed |
description | We report herein development of a novel glass micropipette thermal sensor fabricated in a cost-effective manner, which is capable of measuring steady thermal fluctuation at spatial resolution of ∼2 μm with an accuracy of ±0.01 °C. We produced and tested various micrometer-sized sensors, ranging from 2 μm to 30 μm. The sensor comprises unleaded low-melting-point solder alloy (Sn-based) as a core metal inside a pulled borosilicate glass pipette and a thin film of nickel coating outside, creating a thermocouple junction at the tip. The sensor was calibrated using a thermally insulated calibration chamber, the temperature of which can be controlled with an accuracy of ±0.01 °C, and the thermoelectric power (Seebeck coefficient) of the sensor was recorded from 8.46 to 8.86 μV/°C. We have demonstrated the capability of measuring temperatures at a cellular level by inserting our temperature sensor into the membrane of a live retinal pigment epithelium cell subjected to a laser beam with a focal spot of 6 μm. We measured transient temperature profiles and the maximum temperatures were in the range of 38–55 ± 0.5 °C. |
format | Online Article Text |
id | pubmed-3231465 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-32314652011-12-07 A High-Precision Micropipette Sensor for Cellular-Level Real-Time Thermal Characterization Shrestha, Ramesh Choi, Tae-Youl Chang, Wonseok Kim, Donsik Sensors (Basel) Article We report herein development of a novel glass micropipette thermal sensor fabricated in a cost-effective manner, which is capable of measuring steady thermal fluctuation at spatial resolution of ∼2 μm with an accuracy of ±0.01 °C. We produced and tested various micrometer-sized sensors, ranging from 2 μm to 30 μm. The sensor comprises unleaded low-melting-point solder alloy (Sn-based) as a core metal inside a pulled borosilicate glass pipette and a thin film of nickel coating outside, creating a thermocouple junction at the tip. The sensor was calibrated using a thermally insulated calibration chamber, the temperature of which can be controlled with an accuracy of ±0.01 °C, and the thermoelectric power (Seebeck coefficient) of the sensor was recorded from 8.46 to 8.86 μV/°C. We have demonstrated the capability of measuring temperatures at a cellular level by inserting our temperature sensor into the membrane of a live retinal pigment epithelium cell subjected to a laser beam with a focal spot of 6 μm. We measured transient temperature profiles and the maximum temperatures were in the range of 38–55 ± 0.5 °C. Molecular Diversity Preservation International (MDPI) 2011-09-13 /pmc/articles/PMC3231465/ /pubmed/22164108 http://dx.doi.org/10.3390/s110908826 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Shrestha, Ramesh Choi, Tae-Youl Chang, Wonseok Kim, Donsik A High-Precision Micropipette Sensor for Cellular-Level Real-Time Thermal Characterization |
title | A High-Precision Micropipette Sensor for Cellular-Level Real-Time Thermal Characterization |
title_full | A High-Precision Micropipette Sensor for Cellular-Level Real-Time Thermal Characterization |
title_fullStr | A High-Precision Micropipette Sensor for Cellular-Level Real-Time Thermal Characterization |
title_full_unstemmed | A High-Precision Micropipette Sensor for Cellular-Level Real-Time Thermal Characterization |
title_short | A High-Precision Micropipette Sensor for Cellular-Level Real-Time Thermal Characterization |
title_sort | high-precision micropipette sensor for cellular-level real-time thermal characterization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3231465/ https://www.ncbi.nlm.nih.gov/pubmed/22164108 http://dx.doi.org/10.3390/s110908826 |
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