Branched Intermediate Formation Is the Slowest Step in the Protein Splicing Reaction of the Ala1 KlbA Intein from Methanococcus jannaschii

[Image: see text] We report the first detailed investigation of the kinetics of protein splicing by the Methanococcus jannaschii KlbA (Mja KlbA) intein. This intein has an N-terminal Ala in place of the nucleophilic Cys or Ser residue that normally initiates splicing but nevertheless splices efficie...

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Detalles Bibliográficos
Autores principales: Saleh, Lana, Southworth, Maurice W., Considine, Nancy, O’Neill, Colleen, Benner, Jack, Bollinger, J. Martin, Perler, Francine B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2011
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3231794/
https://www.ncbi.nlm.nih.gov/pubmed/22026921
http://dx.doi.org/10.1021/bi200810j

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