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Selection for autochthonous bifidobacteial isolates adapted to simulated gastrointestinal fluid

BACKGROUND AND THE PURPOSE OF THE STUDY: Bifidobacterial strains are excessively sensitive to acidic conditions and this can affect their living ability in the stomach and fermented foods, and as a result, restrict their use as live probiotic cultures. The aim of the present study was to obtain bifi...

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Autores principales: Jamalifar, H., Bigdeli, B., Nowroozi, J., Zolfaghari, H.S., Fazeli, M.R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3232091/
https://www.ncbi.nlm.nih.gov/pubmed/22615595
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author Jamalifar, H.
Bigdeli, B.
Nowroozi, J.
Zolfaghari, H.S.
Fazeli, M.R.
author_facet Jamalifar, H.
Bigdeli, B.
Nowroozi, J.
Zolfaghari, H.S.
Fazeli, M.R.
author_sort Jamalifar, H.
collection PubMed
description BACKGROUND AND THE PURPOSE OF THE STUDY: Bifidobacterial strains are excessively sensitive to acidic conditions and this can affect their living ability in the stomach and fermented foods, and as a result, restrict their use as live probiotic cultures. The aim of the present study was to obtain bifidobacterial isolates with augmented tolerance to simulated gastrointestinal condition using cross-protection method. METHODS: Individual bifidobacterial strains were treated in acidic environment and also in media containing bile salts and NaCl. Viability of the acid and acid-bile-NaCl tolerant isolates was further examined in simulated gastric and small intestine by subsequent incubation of the probiotic bacteria in the corresponding media for 120 min. Antipathogenic activities of the adapted isolates were compared with those of the original strains. RESULTS AND MAJOR CONCLUSION: The acid and acid-bile-NaCl adapted isolates showed improved viabilities significantly (p<0.05) in simulated gastric fluid compared to their parent strains. The levels of reduction in bacterial count (Log cfu/ml) of the acid and acid-bile-NaCl adapted isolates obtained in simulated gastric fluid ranged from 0.64–3.06 and 0.36–2.43 logarithmic units after 120 min of incubation. There was no significant difference between the viability of the acid-bile-NaCl-tolerant isolates and the original strains in simulated small intestinal condition except for Bifidobacterium adolescentis (p<0.05). The presence of 15 ml of supernatants of acid-bile-NaCl-adapted isolates and also those of the initial Bifidobacterium strains inhibited pathogenic bacterial growth for 24 hrs. Probiotic bacteria with improved ability to survive in harsh gastrointestinal environment could be obtained by subsequent treatment of the strains in acid, bile salts and NaCl environments.
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spelling pubmed-32320912012-05-21 Selection for autochthonous bifidobacteial isolates adapted to simulated gastrointestinal fluid Jamalifar, H. Bigdeli, B. Nowroozi, J. Zolfaghari, H.S. Fazeli, M.R. Daru Original Article BACKGROUND AND THE PURPOSE OF THE STUDY: Bifidobacterial strains are excessively sensitive to acidic conditions and this can affect their living ability in the stomach and fermented foods, and as a result, restrict their use as live probiotic cultures. The aim of the present study was to obtain bifidobacterial isolates with augmented tolerance to simulated gastrointestinal condition using cross-protection method. METHODS: Individual bifidobacterial strains were treated in acidic environment and also in media containing bile salts and NaCl. Viability of the acid and acid-bile-NaCl tolerant isolates was further examined in simulated gastric and small intestine by subsequent incubation of the probiotic bacteria in the corresponding media for 120 min. Antipathogenic activities of the adapted isolates were compared with those of the original strains. RESULTS AND MAJOR CONCLUSION: The acid and acid-bile-NaCl adapted isolates showed improved viabilities significantly (p<0.05) in simulated gastric fluid compared to their parent strains. The levels of reduction in bacterial count (Log cfu/ml) of the acid and acid-bile-NaCl adapted isolates obtained in simulated gastric fluid ranged from 0.64–3.06 and 0.36–2.43 logarithmic units after 120 min of incubation. There was no significant difference between the viability of the acid-bile-NaCl-tolerant isolates and the original strains in simulated small intestinal condition except for Bifidobacterium adolescentis (p<0.05). The presence of 15 ml of supernatants of acid-bile-NaCl-adapted isolates and also those of the initial Bifidobacterium strains inhibited pathogenic bacterial growth for 24 hrs. Probiotic bacteria with improved ability to survive in harsh gastrointestinal environment could be obtained by subsequent treatment of the strains in acid, bile salts and NaCl environments. Tehran University of Medical Sciences 2010 /pmc/articles/PMC3232091/ /pubmed/22615595 Text en © 2010 Tehran University of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Jamalifar, H.
Bigdeli, B.
Nowroozi, J.
Zolfaghari, H.S.
Fazeli, M.R.
Selection for autochthonous bifidobacteial isolates adapted to simulated gastrointestinal fluid
title Selection for autochthonous bifidobacteial isolates adapted to simulated gastrointestinal fluid
title_full Selection for autochthonous bifidobacteial isolates adapted to simulated gastrointestinal fluid
title_fullStr Selection for autochthonous bifidobacteial isolates adapted to simulated gastrointestinal fluid
title_full_unstemmed Selection for autochthonous bifidobacteial isolates adapted to simulated gastrointestinal fluid
title_short Selection for autochthonous bifidobacteial isolates adapted to simulated gastrointestinal fluid
title_sort selection for autochthonous bifidobacteial isolates adapted to simulated gastrointestinal fluid
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3232091/
https://www.ncbi.nlm.nih.gov/pubmed/22615595
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