Seleno-detergent MAD phasing of leukotriene C(4) synthase in complex with dodecyl-β-d-selenomaltoside

Dodecyl-β-d-selenomaltoside (SeDDM) is a seleno-detergent with a β-glycosidic seleno-ether in place of the ether moiety in dodecyl-β-d-maltoside. Seleno-detergents are candidates for heavy-atom agents in experimental phasing of membrane proteins in protein crystallography. Crystals of a nuclear memb...

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Autores principales: Saino, Hiromichi, Ago, Hideo, Ukita, Yoko, Miyano, Masashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2011
Materias:
Laboratory Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3232166/
https://www.ncbi.nlm.nih.gov/pubmed/22139193
http://dx.doi.org/10.1107/S1744309111042345
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author Saino, Hiromichi
Ago, Hideo
Ukita, Yoko
Miyano, Masashi
author_facet Saino, Hiromichi
Ago, Hideo
Ukita, Yoko
Miyano, Masashi
author_sort Saino, Hiromichi
collection PubMed
description Dodecyl-β-d-selenomaltoside (SeDDM) is a seleno-detergent with a β-glycosidic seleno-ether in place of the ether moiety in dodecyl-β-d-maltoside. Seleno-detergents are candidates for heavy-atom agents in experimental phasing of membrane proteins in protein crystallography. Crystals of a nuclear membrane-embedded enzyme, leukotriene C(4) synthase (LTC(4)S), in complex with SeDDM were prepared and a multiwavelength anomalous diffraction (MAD) experiment was performed. The SeDDM in the LTC(4)S crystal exhibited sufficient anomalous diffraction for determination of the structure using MAD phasing.
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spelling pubmed-32321662011-12-12 Seleno-detergent MAD phasing of leukotriene C(4) synthase in complex with dodecyl-β-d-selenomaltoside Saino, Hiromichi Ago, Hideo Ukita, Yoko Miyano, Masashi Acta Crystallogr Sect F Struct Biol Cryst Commun Laboratory Communications Dodecyl-β-d-selenomaltoside (SeDDM) is a seleno-detergent with a β-glycosidic seleno-ether in place of the ether moiety in dodecyl-β-d-maltoside. Seleno-detergents are candidates for heavy-atom agents in experimental phasing of membrane proteins in protein crystallography. Crystals of a nuclear membrane-embedded enzyme, leukotriene C(4) synthase (LTC(4)S), in complex with SeDDM were prepared and a multiwavelength anomalous diffraction (MAD) experiment was performed. The SeDDM in the LTC(4)S crystal exhibited sufficient anomalous diffraction for determination of the structure using MAD phasing. International Union of Crystallography 2011-11-29 /pmc/articles/PMC3232166/ /pubmed/22139193 http://dx.doi.org/10.1107/S1744309111042345 Text en © Saino et al. 2011 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Laboratory Communications
Saino, Hiromichi
Ago, Hideo
Ukita, Yoko
Miyano, Masashi
Seleno-detergent MAD phasing of leukotriene C(4) synthase in complex with dodecyl-β-d-selenomaltoside
title Seleno-detergent MAD phasing of leukotriene C(4) synthase in complex with dodecyl-β-d-selenomaltoside
title_full Seleno-detergent MAD phasing of leukotriene C(4) synthase in complex with dodecyl-β-d-selenomaltoside
title_fullStr Seleno-detergent MAD phasing of leukotriene C(4) synthase in complex with dodecyl-β-d-selenomaltoside
title_full_unstemmed Seleno-detergent MAD phasing of leukotriene C(4) synthase in complex with dodecyl-β-d-selenomaltoside
title_short Seleno-detergent MAD phasing of leukotriene C(4) synthase in complex with dodecyl-β-d-selenomaltoside
title_sort seleno-detergent mad phasing of leukotriene c(4) synthase in complex with dodecyl-β-d-selenomaltoside
topic Laboratory Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3232166/
https://www.ncbi.nlm.nih.gov/pubmed/22139193
http://dx.doi.org/10.1107/S1744309111042345
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