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Biodynamic parameters of micellar diminazene in sheep erythrocytes and blood plasma

In this work, we used a preparation of diminazene, which belongs to the group of aromatic diamidines. This compound acts on the causative agents of blood protozoan diseases produced by both flagellated protozoa (Trypanosoma) and members of the class Piroplasmida (Babesia, Theileria, and Cytauxzoon)...

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Autores principales: Staroverov, Sergey A., Sidorkin, Vladimir A., Fomin, Alexander S., Shchyogolev, Sergey Yu., Dykman, Lev A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3232388/
https://www.ncbi.nlm.nih.gov/pubmed/22122895
http://dx.doi.org/10.4142/jvs.2011.12.4.303
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author Staroverov, Sergey A.
Sidorkin, Vladimir A.
Fomin, Alexander S.
Shchyogolev, Sergey Yu.
Dykman, Lev A.
author_facet Staroverov, Sergey A.
Sidorkin, Vladimir A.
Fomin, Alexander S.
Shchyogolev, Sergey Yu.
Dykman, Lev A.
author_sort Staroverov, Sergey A.
collection PubMed
description In this work, we used a preparation of diminazene, which belongs to the group of aromatic diamidines. This compound acts on the causative agents of blood protozoan diseases produced by both flagellated protozoa (Trypanosoma) and members of the class Piroplasmida (Babesia, Theileria, and Cytauxzoon) in various domestic and wild animals, and it is widely used in veterinary medicine. We examined the behavior of water-disperse diminazene (immobilized in Tween 80 micelles) at the cellular and organismal levels. We assessed the interaction of an aqueous and a water-disperse preparation with cells of the reticuloendothelial system. We compared the kinetic parameters of aqueous and water-disperse diminazene in sheep erythrocytes and plasma. The therapeutic properties of these two preparations were also compared. We found that the surface-active substances improved intracellular penetration of the active substance through interaction with the cell membrane. In sheep blood erythrocytes, micellar diminazene accumulated more than its aqueous analog. This form was also more effective therapeutically than the aqueous analog. Our findings demonstrate that use of micellar diminazene allows the injection dose to be reduced by 30%.
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spelling pubmed-32323882011-12-08 Biodynamic parameters of micellar diminazene in sheep erythrocytes and blood plasma Staroverov, Sergey A. Sidorkin, Vladimir A. Fomin, Alexander S. Shchyogolev, Sergey Yu. Dykman, Lev A. J Vet Sci Original Article In this work, we used a preparation of diminazene, which belongs to the group of aromatic diamidines. This compound acts on the causative agents of blood protozoan diseases produced by both flagellated protozoa (Trypanosoma) and members of the class Piroplasmida (Babesia, Theileria, and Cytauxzoon) in various domestic and wild animals, and it is widely used in veterinary medicine. We examined the behavior of water-disperse diminazene (immobilized in Tween 80 micelles) at the cellular and organismal levels. We assessed the interaction of an aqueous and a water-disperse preparation with cells of the reticuloendothelial system. We compared the kinetic parameters of aqueous and water-disperse diminazene in sheep erythrocytes and plasma. The therapeutic properties of these two preparations were also compared. We found that the surface-active substances improved intracellular penetration of the active substance through interaction with the cell membrane. In sheep blood erythrocytes, micellar diminazene accumulated more than its aqueous analog. This form was also more effective therapeutically than the aqueous analog. Our findings demonstrate that use of micellar diminazene allows the injection dose to be reduced by 30%. The Korean Society of Veterinary Science 2011-12 2011-11-30 /pmc/articles/PMC3232388/ /pubmed/22122895 http://dx.doi.org/10.4142/jvs.2011.12.4.303 Text en Copyright © 2011 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Staroverov, Sergey A.
Sidorkin, Vladimir A.
Fomin, Alexander S.
Shchyogolev, Sergey Yu.
Dykman, Lev A.
Biodynamic parameters of micellar diminazene in sheep erythrocytes and blood plasma
title Biodynamic parameters of micellar diminazene in sheep erythrocytes and blood plasma
title_full Biodynamic parameters of micellar diminazene in sheep erythrocytes and blood plasma
title_fullStr Biodynamic parameters of micellar diminazene in sheep erythrocytes and blood plasma
title_full_unstemmed Biodynamic parameters of micellar diminazene in sheep erythrocytes and blood plasma
title_short Biodynamic parameters of micellar diminazene in sheep erythrocytes and blood plasma
title_sort biodynamic parameters of micellar diminazene in sheep erythrocytes and blood plasma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3232388/
https://www.ncbi.nlm.nih.gov/pubmed/22122895
http://dx.doi.org/10.4142/jvs.2011.12.4.303
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