Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate

Corn, one of the most important forage crops worldwide, has proven to be a useful expression vehicle due to the availability of established transformation procedures for this well-studied plant. The exotoxin Apx, a major virulence factor, is recognized as a common antigen of Actinobacillus (A.) pleu...

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Autores principales: Shin, Min-Kyoung, Jung, Myung Hwan, Lee, Won-Jung, Choi, Pil Son, Jang, Yong-Suk, Yoo, Han Sang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2011
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3232401/
https://www.ncbi.nlm.nih.gov/pubmed/22122907
http://dx.doi.org/10.4142/jvs.2011.12.4.401
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author Shin, Min-Kyoung
Jung, Myung Hwan
Lee, Won-Jung
Choi, Pil Son
Jang, Yong-Suk
Yoo, Han Sang
author_facet Shin, Min-Kyoung
Jung, Myung Hwan
Lee, Won-Jung
Choi, Pil Son
Jang, Yong-Suk
Yoo, Han Sang
author_sort Shin, Min-Kyoung
collection PubMed
description Corn, one of the most important forage crops worldwide, has proven to be a useful expression vehicle due to the availability of established transformation procedures for this well-studied plant. The exotoxin Apx, a major virulence factor, is recognized as a common antigen of Actinobacillus (A.) pleuropneumoniae, the causative agent of porcine pleuropneumonia. In this study, a cholera toxin B (CTB)-ApxIIA#5 fusion protein and full-size ApxIIA expressed in corn seed, as a subunit vaccine candidate, were observed to induce Apx-specific immune responses in mice. These results suggest that transgenic corn-derived ApxIIA and CTB-ApxIIA#5 proteins are potential vaccine candidates against A. pleuropneumoniae infection.
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spelling pubmed-32324012011-12-08 Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate Shin, Min-Kyoung Jung, Myung Hwan Lee, Won-Jung Choi, Pil Son Jang, Yong-Suk Yoo, Han Sang J Vet Sci Short Communication Corn, one of the most important forage crops worldwide, has proven to be a useful expression vehicle due to the availability of established transformation procedures for this well-studied plant. The exotoxin Apx, a major virulence factor, is recognized as a common antigen of Actinobacillus (A.) pleuropneumoniae, the causative agent of porcine pleuropneumonia. In this study, a cholera toxin B (CTB)-ApxIIA#5 fusion protein and full-size ApxIIA expressed in corn seed, as a subunit vaccine candidate, were observed to induce Apx-specific immune responses in mice. These results suggest that transgenic corn-derived ApxIIA and CTB-ApxIIA#5 proteins are potential vaccine candidates against A. pleuropneumoniae infection. The Korean Society of Veterinary Science 2011-12 2011-11-30 /pmc/articles/PMC3232401/ /pubmed/22122907 http://dx.doi.org/10.4142/jvs.2011.12.4.401 Text en Copyright © 2011 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Shin, Min-Kyoung
Jung, Myung Hwan
Lee, Won-Jung
Choi, Pil Son
Jang, Yong-Suk
Yoo, Han Sang
Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate
title Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate
title_full Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate
title_fullStr Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate
title_full_unstemmed Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate
title_short Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate
title_sort generation of transgenic corn-derived actinobacillus pleuropneumoniae apxiia fused with the cholera toxin b subunit as a vaccine candidate
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3232401/
https://www.ncbi.nlm.nih.gov/pubmed/22122907
http://dx.doi.org/10.4142/jvs.2011.12.4.401
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