Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging

We have developed a fiber-based, video-rate fluorescence diffuse optical tomography (DOT) system for noninvasive in vivo sentinel lymph node (SLN) mapping. Concurrent acquisition of fluorescence and reference signals allowed the efficient generation of ratio-metric data for 3D image reconstruction....

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Detalles Bibliográficos
Autores principales: Solomon, Metasebya, White, Brian R., Nothdruft, Ralph E., Akers, Walter, Sudlow, Gail, Eggebrecht, Adam T., Achilefu, Samuel, Culver, Joseph P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Diffuse Optical Imaging
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233246/
https://www.ncbi.nlm.nih.gov/pubmed/22162817
http://dx.doi.org/10.1364/BOE.2.003267
Descripción
Sumario:We have developed a fiber-based, video-rate fluorescence diffuse optical tomography (DOT) system for noninvasive in vivo sentinel lymph node (SLN) mapping. Concurrent acquisition of fluorescence and reference signals allowed the efficient generation of ratio-metric data for 3D image reconstruction. Accurate depth localization and high sensitivity to fluorescent targets were established in to depths of >10 mm. In vivo accumulation of indocyanine green (ICG) dye was imaged in the region of the SLN following intradermal injection into the forepaw of rats. These results suggest that video-rate fluorescence DOT has significant potential as a clinical tool for noninvasive mapping of SLN.

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