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Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging
We have developed a fiber-based, video-rate fluorescence diffuse optical tomography (DOT) system for noninvasive in vivo sentinel lymph node (SLN) mapping. Concurrent acquisition of fluorescence and reference signals allowed the efficient generation of ratio-metric data for 3D image reconstruction....
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233246/ https://www.ncbi.nlm.nih.gov/pubmed/22162817 http://dx.doi.org/10.1364/BOE.2.003267 |
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author | Solomon, Metasebya White, Brian R. Nothdruft, Ralph E. Akers, Walter Sudlow, Gail Eggebrecht, Adam T. Achilefu, Samuel Culver, Joseph P. |
author_facet | Solomon, Metasebya White, Brian R. Nothdruft, Ralph E. Akers, Walter Sudlow, Gail Eggebrecht, Adam T. Achilefu, Samuel Culver, Joseph P. |
author_sort | Solomon, Metasebya |
collection | PubMed |
description | We have developed a fiber-based, video-rate fluorescence diffuse optical tomography (DOT) system for noninvasive in vivo sentinel lymph node (SLN) mapping. Concurrent acquisition of fluorescence and reference signals allowed the efficient generation of ratio-metric data for 3D image reconstruction. Accurate depth localization and high sensitivity to fluorescent targets were established in to depths of >10 mm. In vivo accumulation of indocyanine green (ICG) dye was imaged in the region of the SLN following intradermal injection into the forepaw of rats. These results suggest that video-rate fluorescence DOT has significant potential as a clinical tool for noninvasive mapping of SLN. |
format | Online Article Text |
id | pubmed-3233246 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-32332462011-12-08 Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging Solomon, Metasebya White, Brian R. Nothdruft, Ralph E. Akers, Walter Sudlow, Gail Eggebrecht, Adam T. Achilefu, Samuel Culver, Joseph P. Biomed Opt Express Diffuse Optical Imaging We have developed a fiber-based, video-rate fluorescence diffuse optical tomography (DOT) system for noninvasive in vivo sentinel lymph node (SLN) mapping. Concurrent acquisition of fluorescence and reference signals allowed the efficient generation of ratio-metric data for 3D image reconstruction. Accurate depth localization and high sensitivity to fluorescent targets were established in to depths of >10 mm. In vivo accumulation of indocyanine green (ICG) dye was imaged in the region of the SLN following intradermal injection into the forepaw of rats. These results suggest that video-rate fluorescence DOT has significant potential as a clinical tool for noninvasive mapping of SLN. Optical Society of America 2011-11-08 /pmc/articles/PMC3233246/ /pubmed/22162817 http://dx.doi.org/10.1364/BOE.2.003267 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Diffuse Optical Imaging Solomon, Metasebya White, Brian R. Nothdruft, Ralph E. Akers, Walter Sudlow, Gail Eggebrecht, Adam T. Achilefu, Samuel Culver, Joseph P. Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging |
title | Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging |
title_full | Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging |
title_fullStr | Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging |
title_full_unstemmed | Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging |
title_short | Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging |
title_sort | video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging |
topic | Diffuse Optical Imaging |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233246/ https://www.ncbi.nlm.nih.gov/pubmed/22162817 http://dx.doi.org/10.1364/BOE.2.003267 |
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