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Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging

We have developed a fiber-based, video-rate fluorescence diffuse optical tomography (DOT) system for noninvasive in vivo sentinel lymph node (SLN) mapping. Concurrent acquisition of fluorescence and reference signals allowed the efficient generation of ratio-metric data for 3D image reconstruction....

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Detalles Bibliográficos
Autores principales: Solomon, Metasebya, White, Brian R., Nothdruft, Ralph E., Akers, Walter, Sudlow, Gail, Eggebrecht, Adam T., Achilefu, Samuel, Culver, Joseph P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233246/
https://www.ncbi.nlm.nih.gov/pubmed/22162817
http://dx.doi.org/10.1364/BOE.2.003267
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author Solomon, Metasebya
White, Brian R.
Nothdruft, Ralph E.
Akers, Walter
Sudlow, Gail
Eggebrecht, Adam T.
Achilefu, Samuel
Culver, Joseph P.
author_facet Solomon, Metasebya
White, Brian R.
Nothdruft, Ralph E.
Akers, Walter
Sudlow, Gail
Eggebrecht, Adam T.
Achilefu, Samuel
Culver, Joseph P.
author_sort Solomon, Metasebya
collection PubMed
description We have developed a fiber-based, video-rate fluorescence diffuse optical tomography (DOT) system for noninvasive in vivo sentinel lymph node (SLN) mapping. Concurrent acquisition of fluorescence and reference signals allowed the efficient generation of ratio-metric data for 3D image reconstruction. Accurate depth localization and high sensitivity to fluorescent targets were established in to depths of >10 mm. In vivo accumulation of indocyanine green (ICG) dye was imaged in the region of the SLN following intradermal injection into the forepaw of rats. These results suggest that video-rate fluorescence DOT has significant potential as a clinical tool for noninvasive mapping of SLN.
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spelling pubmed-32332462011-12-08 Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging Solomon, Metasebya White, Brian R. Nothdruft, Ralph E. Akers, Walter Sudlow, Gail Eggebrecht, Adam T. Achilefu, Samuel Culver, Joseph P. Biomed Opt Express Diffuse Optical Imaging We have developed a fiber-based, video-rate fluorescence diffuse optical tomography (DOT) system for noninvasive in vivo sentinel lymph node (SLN) mapping. Concurrent acquisition of fluorescence and reference signals allowed the efficient generation of ratio-metric data for 3D image reconstruction. Accurate depth localization and high sensitivity to fluorescent targets were established in to depths of >10 mm. In vivo accumulation of indocyanine green (ICG) dye was imaged in the region of the SLN following intradermal injection into the forepaw of rats. These results suggest that video-rate fluorescence DOT has significant potential as a clinical tool for noninvasive mapping of SLN. Optical Society of America 2011-11-08 /pmc/articles/PMC3233246/ /pubmed/22162817 http://dx.doi.org/10.1364/BOE.2.003267 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Diffuse Optical Imaging
Solomon, Metasebya
White, Brian R.
Nothdruft, Ralph E.
Akers, Walter
Sudlow, Gail
Eggebrecht, Adam T.
Achilefu, Samuel
Culver, Joseph P.
Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging
title Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging
title_full Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging
title_fullStr Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging
title_full_unstemmed Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging
title_short Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging
title_sort video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging
topic Diffuse Optical Imaging
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233246/
https://www.ncbi.nlm.nih.gov/pubmed/22162817
http://dx.doi.org/10.1364/BOE.2.003267
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