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Noninvasive detection of filaggrin gene mutations using Raman spectroscopy

Knowledge of the existence of filaggrin (FLG) gene mutations might be helpful for a subclassification of patients with atopic dermatitis (AD) which can be used to introduce individualized treatments. In this work the filaggrin content in the skin is assessed using Raman spectroscopy and the results...

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Autores principales: González, Francisco J., Valdes-Rodríguez, Rodrigo, Ramírez-Elías, Miguel G., Castillo-Martínez, Claudio, Saavedra-Alanis, Victor M., Moncada, Benjamín
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233254/
https://www.ncbi.nlm.nih.gov/pubmed/22162825
http://dx.doi.org/10.1364/BOE.2.003363
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author González, Francisco J.
Valdes-Rodríguez, Rodrigo
Ramírez-Elías, Miguel G.
Castillo-Martínez, Claudio
Saavedra-Alanis, Victor M.
Moncada, Benjamín
author_facet González, Francisco J.
Valdes-Rodríguez, Rodrigo
Ramírez-Elías, Miguel G.
Castillo-Martínez, Claudio
Saavedra-Alanis, Victor M.
Moncada, Benjamín
author_sort González, Francisco J.
collection PubMed
description Knowledge of the existence of filaggrin (FLG) gene mutations might be helpful for a subclassification of patients with atopic dermatitis (AD) which can be used to introduce individualized treatments. In this work the filaggrin content in the skin is assessed using Raman spectroscopy and the results are compared to FLG genotyping of Mexican-mestizo patients. Results showed that the 2282del4 and R501X mutations present in the European population but absent in people of Asian or African descent are also present in the Mexican-mestizo population. The results also showed that patients with filaggrin gene mutations presented lower filaggrin concentrations measured using the vector correlation of their skin Raman spectra and a fixed spectrum of pure human recombinant filaggrin, these results indicate that Raman spectroscopy may be used as a noninvasive tool to detect FLG gene mutations.
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spelling pubmed-32332542011-12-08 Noninvasive detection of filaggrin gene mutations using Raman spectroscopy González, Francisco J. Valdes-Rodríguez, Rodrigo Ramírez-Elías, Miguel G. Castillo-Martínez, Claudio Saavedra-Alanis, Victor M. Moncada, Benjamín Biomed Opt Express Dermatological Applications Knowledge of the existence of filaggrin (FLG) gene mutations might be helpful for a subclassification of patients with atopic dermatitis (AD) which can be used to introduce individualized treatments. In this work the filaggrin content in the skin is assessed using Raman spectroscopy and the results are compared to FLG genotyping of Mexican-mestizo patients. Results showed that the 2282del4 and R501X mutations present in the European population but absent in people of Asian or African descent are also present in the Mexican-mestizo population. The results also showed that patients with filaggrin gene mutations presented lower filaggrin concentrations measured using the vector correlation of their skin Raman spectra and a fixed spectrum of pure human recombinant filaggrin, these results indicate that Raman spectroscopy may be used as a noninvasive tool to detect FLG gene mutations. Optical Society of America 2011-11-28 /pmc/articles/PMC3233254/ /pubmed/22162825 http://dx.doi.org/10.1364/BOE.2.003363 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Dermatological Applications
González, Francisco J.
Valdes-Rodríguez, Rodrigo
Ramírez-Elías, Miguel G.
Castillo-Martínez, Claudio
Saavedra-Alanis, Victor M.
Moncada, Benjamín
Noninvasive detection of filaggrin gene mutations using Raman spectroscopy
title Noninvasive detection of filaggrin gene mutations using Raman spectroscopy
title_full Noninvasive detection of filaggrin gene mutations using Raman spectroscopy
title_fullStr Noninvasive detection of filaggrin gene mutations using Raman spectroscopy
title_full_unstemmed Noninvasive detection of filaggrin gene mutations using Raman spectroscopy
title_short Noninvasive detection of filaggrin gene mutations using Raman spectroscopy
title_sort noninvasive detection of filaggrin gene mutations using raman spectroscopy
topic Dermatological Applications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233254/
https://www.ncbi.nlm.nih.gov/pubmed/22162825
http://dx.doi.org/10.1364/BOE.2.003363
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