Cargando…
Noninvasive detection of filaggrin gene mutations using Raman spectroscopy
Knowledge of the existence of filaggrin (FLG) gene mutations might be helpful for a subclassification of patients with atopic dermatitis (AD) which can be used to introduce individualized treatments. In this work the filaggrin content in the skin is assessed using Raman spectroscopy and the results...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233254/ https://www.ncbi.nlm.nih.gov/pubmed/22162825 http://dx.doi.org/10.1364/BOE.2.003363 |
_version_ | 1782218391984013312 |
---|---|
author | González, Francisco J. Valdes-Rodríguez, Rodrigo Ramírez-Elías, Miguel G. Castillo-Martínez, Claudio Saavedra-Alanis, Victor M. Moncada, Benjamín |
author_facet | González, Francisco J. Valdes-Rodríguez, Rodrigo Ramírez-Elías, Miguel G. Castillo-Martínez, Claudio Saavedra-Alanis, Victor M. Moncada, Benjamín |
author_sort | González, Francisco J. |
collection | PubMed |
description | Knowledge of the existence of filaggrin (FLG) gene mutations might be helpful for a subclassification of patients with atopic dermatitis (AD) which can be used to introduce individualized treatments. In this work the filaggrin content in the skin is assessed using Raman spectroscopy and the results are compared to FLG genotyping of Mexican-mestizo patients. Results showed that the 2282del4 and R501X mutations present in the European population but absent in people of Asian or African descent are also present in the Mexican-mestizo population. The results also showed that patients with filaggrin gene mutations presented lower filaggrin concentrations measured using the vector correlation of their skin Raman spectra and a fixed spectrum of pure human recombinant filaggrin, these results indicate that Raman spectroscopy may be used as a noninvasive tool to detect FLG gene mutations. |
format | Online Article Text |
id | pubmed-3233254 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-32332542011-12-08 Noninvasive detection of filaggrin gene mutations using Raman spectroscopy González, Francisco J. Valdes-Rodríguez, Rodrigo Ramírez-Elías, Miguel G. Castillo-Martínez, Claudio Saavedra-Alanis, Victor M. Moncada, Benjamín Biomed Opt Express Dermatological Applications Knowledge of the existence of filaggrin (FLG) gene mutations might be helpful for a subclassification of patients with atopic dermatitis (AD) which can be used to introduce individualized treatments. In this work the filaggrin content in the skin is assessed using Raman spectroscopy and the results are compared to FLG genotyping of Mexican-mestizo patients. Results showed that the 2282del4 and R501X mutations present in the European population but absent in people of Asian or African descent are also present in the Mexican-mestizo population. The results also showed that patients with filaggrin gene mutations presented lower filaggrin concentrations measured using the vector correlation of their skin Raman spectra and a fixed spectrum of pure human recombinant filaggrin, these results indicate that Raman spectroscopy may be used as a noninvasive tool to detect FLG gene mutations. Optical Society of America 2011-11-28 /pmc/articles/PMC3233254/ /pubmed/22162825 http://dx.doi.org/10.1364/BOE.2.003363 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Dermatological Applications González, Francisco J. Valdes-Rodríguez, Rodrigo Ramírez-Elías, Miguel G. Castillo-Martínez, Claudio Saavedra-Alanis, Victor M. Moncada, Benjamín Noninvasive detection of filaggrin gene mutations using Raman spectroscopy |
title | Noninvasive detection of filaggrin gene mutations using Raman spectroscopy |
title_full | Noninvasive detection of filaggrin gene mutations using Raman spectroscopy |
title_fullStr | Noninvasive detection of filaggrin gene mutations using Raman spectroscopy |
title_full_unstemmed | Noninvasive detection of filaggrin gene mutations using Raman spectroscopy |
title_short | Noninvasive detection of filaggrin gene mutations using Raman spectroscopy |
title_sort | noninvasive detection of filaggrin gene mutations using raman spectroscopy |
topic | Dermatological Applications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233254/ https://www.ncbi.nlm.nih.gov/pubmed/22162825 http://dx.doi.org/10.1364/BOE.2.003363 |
work_keys_str_mv | AT gonzalezfranciscoj noninvasivedetectionoffilaggringenemutationsusingramanspectroscopy AT valdesrodriguezrodrigo noninvasivedetectionoffilaggringenemutationsusingramanspectroscopy AT ramirezeliasmiguelg noninvasivedetectionoffilaggringenemutationsusingramanspectroscopy AT castillomartinezclaudio noninvasivedetectionoffilaggringenemutationsusingramanspectroscopy AT saavedraalanisvictorm noninvasivedetectionoffilaggringenemutationsusingramanspectroscopy AT moncadabenjamin noninvasivedetectionoffilaggringenemutationsusingramanspectroscopy |