Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells
We previously isolated aaptamine, a benzonaphthyridine alkaloid, from marine sponge Aaptos suberitoids. In this study, we investigated the anti-proliferative effect of aaptamine on chronic myeloid leukemia (CML) K562 cells. Aaptamine inhibited growth of K562 with a GI50 as 10 μM, and arrested cell c...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233409/ https://www.ncbi.nlm.nih.gov/pubmed/22174603 http://dx.doi.org/10.3390/ijms12117352 |
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author | Jin, Meihua Zhao, Wennan Zhang, Yanwen Kobayashi, Motomasa Duan, Hongquan Kong, Dexin |
author_facet | Jin, Meihua Zhao, Wennan Zhang, Yanwen Kobayashi, Motomasa Duan, Hongquan Kong, Dexin |
author_sort | Jin, Meihua |
collection | PubMed |
description | We previously isolated aaptamine, a benzonaphthyridine alkaloid, from marine sponge Aaptos suberitoids. In this study, we investigated the anti-proliferative effect of aaptamine on chronic myeloid leukemia (CML) K562 cells. Aaptamine inhibited growth of K562 with a GI50 as 10 μM, and arrested cell cycle at G2/M phase. Western blot analysis indicated that aaptamine induced p21 expression in K562 cells. Moreover, p21 promoter was activated by aaptamine treatment in p21 transfected K562 cells. Since K562 is p53 negative, aaptamine was demonstrated to be a p53-independent p21 inducer in CML cells. |
format | Online Article Text |
id | pubmed-3233409 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-32334092011-12-15 Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells Jin, Meihua Zhao, Wennan Zhang, Yanwen Kobayashi, Motomasa Duan, Hongquan Kong, Dexin Int J Mol Sci Article We previously isolated aaptamine, a benzonaphthyridine alkaloid, from marine sponge Aaptos suberitoids. In this study, we investigated the anti-proliferative effect of aaptamine on chronic myeloid leukemia (CML) K562 cells. Aaptamine inhibited growth of K562 with a GI50 as 10 μM, and arrested cell cycle at G2/M phase. Western blot analysis indicated that aaptamine induced p21 expression in K562 cells. Moreover, p21 promoter was activated by aaptamine treatment in p21 transfected K562 cells. Since K562 is p53 negative, aaptamine was demonstrated to be a p53-independent p21 inducer in CML cells. Molecular Diversity Preservation International (MDPI) 2011-10-26 /pmc/articles/PMC3233409/ /pubmed/22174603 http://dx.doi.org/10.3390/ijms12117352 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Jin, Meihua Zhao, Wennan Zhang, Yanwen Kobayashi, Motomasa Duan, Hongquan Kong, Dexin Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells |
title | Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells |
title_full | Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells |
title_fullStr | Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells |
title_full_unstemmed | Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells |
title_short | Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells |
title_sort | antiproliferative effect of aaptamine on human chronic myeloid leukemia k562 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233409/ https://www.ncbi.nlm.nih.gov/pubmed/22174603 http://dx.doi.org/10.3390/ijms12117352 |
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