Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells

We previously isolated aaptamine, a benzonaphthyridine alkaloid, from marine sponge Aaptos suberitoids. In this study, we investigated the anti-proliferative effect of aaptamine on chronic myeloid leukemia (CML) K562 cells. Aaptamine inhibited growth of K562 with a GI50 as 10 μM, and arrested cell c...

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Autores principales: Jin, Meihua, Zhao, Wennan, Zhang, Yanwen, Kobayashi, Motomasa, Duan, Hongquan, Kong, Dexin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233409/
https://www.ncbi.nlm.nih.gov/pubmed/22174603
http://dx.doi.org/10.3390/ijms12117352
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author Jin, Meihua
Zhao, Wennan
Zhang, Yanwen
Kobayashi, Motomasa
Duan, Hongquan
Kong, Dexin
author_facet Jin, Meihua
Zhao, Wennan
Zhang, Yanwen
Kobayashi, Motomasa
Duan, Hongquan
Kong, Dexin
author_sort Jin, Meihua
collection PubMed
description We previously isolated aaptamine, a benzonaphthyridine alkaloid, from marine sponge Aaptos suberitoids. In this study, we investigated the anti-proliferative effect of aaptamine on chronic myeloid leukemia (CML) K562 cells. Aaptamine inhibited growth of K562 with a GI50 as 10 μM, and arrested cell cycle at G2/M phase. Western blot analysis indicated that aaptamine induced p21 expression in K562 cells. Moreover, p21 promoter was activated by aaptamine treatment in p21 transfected K562 cells. Since K562 is p53 negative, aaptamine was demonstrated to be a p53-independent p21 inducer in CML cells.
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spelling pubmed-32334092011-12-15 Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells Jin, Meihua Zhao, Wennan Zhang, Yanwen Kobayashi, Motomasa Duan, Hongquan Kong, Dexin Int J Mol Sci Article We previously isolated aaptamine, a benzonaphthyridine alkaloid, from marine sponge Aaptos suberitoids. In this study, we investigated the anti-proliferative effect of aaptamine on chronic myeloid leukemia (CML) K562 cells. Aaptamine inhibited growth of K562 with a GI50 as 10 μM, and arrested cell cycle at G2/M phase. Western blot analysis indicated that aaptamine induced p21 expression in K562 cells. Moreover, p21 promoter was activated by aaptamine treatment in p21 transfected K562 cells. Since K562 is p53 negative, aaptamine was demonstrated to be a p53-independent p21 inducer in CML cells. Molecular Diversity Preservation International (MDPI) 2011-10-26 /pmc/articles/PMC3233409/ /pubmed/22174603 http://dx.doi.org/10.3390/ijms12117352 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Jin, Meihua
Zhao, Wennan
Zhang, Yanwen
Kobayashi, Motomasa
Duan, Hongquan
Kong, Dexin
Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells
title Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells
title_full Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells
title_fullStr Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells
title_full_unstemmed Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells
title_short Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells
title_sort antiproliferative effect of aaptamine on human chronic myeloid leukemia k562 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233409/
https://www.ncbi.nlm.nih.gov/pubmed/22174603
http://dx.doi.org/10.3390/ijms12117352
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