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DNA Sequence Analysis of South African Helicobacter pylori Vacuolating Cytotoxin Gene (vacA)
Sequence diversity and population structures can vary widely among pathogenic bacteria species. In some species, all isolates are highly similar, whereas in others most of the isolates are distinguished easily. H. pylori is known for its wide genetic diversity amongst the various strains most especi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233416/ https://www.ncbi.nlm.nih.gov/pubmed/22174610 http://dx.doi.org/10.3390/ijms12117459 |
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author | Tanih, Nicoline F. Ndip, Lucy M. Ndip, Roland N. |
author_facet | Tanih, Nicoline F. Ndip, Lucy M. Ndip, Roland N. |
author_sort | Tanih, Nicoline F. |
collection | PubMed |
description | Sequence diversity and population structures can vary widely among pathogenic bacteria species. In some species, all isolates are highly similar, whereas in others most of the isolates are distinguished easily. H. pylori is known for its wide genetic diversity amongst the various strains most especially in the genes involved in virulence. The aim of this study was to evaluate by PCR and sequence analysis, the genetic profile of H. pylori vacA gene (s1, s2, m1 and m2). We sequenced small DNA segments from 13 vacAs1, 10 vacAm2, 6 vacAm1 and 6 vacAs2 strains which were amplified with amplicon size of 259/286 bp, 290 bp and 352 bp for vacAs1/s2, m1 and m2 respectively. Based on similarities among our strains accession numbers were provided for seven vacAs1 (HQ709109–HQ709115), six vacAs2 (JN848463–JN848468), six vacAm1 (JN848469–JN848474) and six vacAm2 (HQ650801–HQ650806) strains. Amongst the strains studied, 98.07%, 98.58%, 97.38% and 95.41% of vacAs1, vacAs2, vacAm1 and vacAm2 of the strains were conserved respectively. Findings of this study underscores the importance of understanding the virulence composition and diversity of H. pylori in South Africa for enhanced clinico-epidemiological monitoring and pathophysiology of disease. |
format | Online Article Text |
id | pubmed-3233416 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-32334162011-12-15 DNA Sequence Analysis of South African Helicobacter pylori Vacuolating Cytotoxin Gene (vacA) Tanih, Nicoline F. Ndip, Lucy M. Ndip, Roland N. Int J Mol Sci Article Sequence diversity and population structures can vary widely among pathogenic bacteria species. In some species, all isolates are highly similar, whereas in others most of the isolates are distinguished easily. H. pylori is known for its wide genetic diversity amongst the various strains most especially in the genes involved in virulence. The aim of this study was to evaluate by PCR and sequence analysis, the genetic profile of H. pylori vacA gene (s1, s2, m1 and m2). We sequenced small DNA segments from 13 vacAs1, 10 vacAm2, 6 vacAm1 and 6 vacAs2 strains which were amplified with amplicon size of 259/286 bp, 290 bp and 352 bp for vacAs1/s2, m1 and m2 respectively. Based on similarities among our strains accession numbers were provided for seven vacAs1 (HQ709109–HQ709115), six vacAs2 (JN848463–JN848468), six vacAm1 (JN848469–JN848474) and six vacAm2 (HQ650801–HQ650806) strains. Amongst the strains studied, 98.07%, 98.58%, 97.38% and 95.41% of vacAs1, vacAs2, vacAm1 and vacAm2 of the strains were conserved respectively. Findings of this study underscores the importance of understanding the virulence composition and diversity of H. pylori in South Africa for enhanced clinico-epidemiological monitoring and pathophysiology of disease. Molecular Diversity Preservation International (MDPI) 2011-10-31 /pmc/articles/PMC3233416/ /pubmed/22174610 http://dx.doi.org/10.3390/ijms12117459 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Tanih, Nicoline F. Ndip, Lucy M. Ndip, Roland N. DNA Sequence Analysis of South African Helicobacter pylori Vacuolating Cytotoxin Gene (vacA) |
title | DNA Sequence Analysis of South African Helicobacter pylori Vacuolating Cytotoxin Gene (vacA) |
title_full | DNA Sequence Analysis of South African Helicobacter pylori Vacuolating Cytotoxin Gene (vacA) |
title_fullStr | DNA Sequence Analysis of South African Helicobacter pylori Vacuolating Cytotoxin Gene (vacA) |
title_full_unstemmed | DNA Sequence Analysis of South African Helicobacter pylori Vacuolating Cytotoxin Gene (vacA) |
title_short | DNA Sequence Analysis of South African Helicobacter pylori Vacuolating Cytotoxin Gene (vacA) |
title_sort | dna sequence analysis of south african helicobacter pylori vacuolating cytotoxin gene (vaca) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233416/ https://www.ncbi.nlm.nih.gov/pubmed/22174610 http://dx.doi.org/10.3390/ijms12117459 |
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