3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence

Using UV-vis absorption and circular dichroism (CD) spectroscopies, we explored the binding interactions of 3,3′-diethylthiatricarbocyanine iodide (Cy7) with polynucleotides of different sequences and helicity. CD showed to be a very diagnostic tool giving different spectroscopic chiroptical signatu...

Descripción completa

Detalles Bibliográficos
Autores principales: Choi, Jung Kyu, D’Urso, Alessandro, Trauernicht, Mitch, Shabbir-Hussain, Murtaza, Holmes, Andrea E., Balaz, Milan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233455/
https://www.ncbi.nlm.nih.gov/pubmed/22174649
http://dx.doi.org/10.3390/ijms12118052
_version_ 1782218413191462912
author Choi, Jung Kyu
D’Urso, Alessandro
Trauernicht, Mitch
Shabbir-Hussain, Murtaza
Holmes, Andrea E.
Balaz, Milan
author_facet Choi, Jung Kyu
D’Urso, Alessandro
Trauernicht, Mitch
Shabbir-Hussain, Murtaza
Holmes, Andrea E.
Balaz, Milan
author_sort Choi, Jung Kyu
collection PubMed
description Using UV-vis absorption and circular dichroism (CD) spectroscopies, we explored the binding interactions of 3,3′-diethylthiatricarbocyanine iodide (Cy7) with polynucleotides of different sequences and helicity. CD showed to be a very diagnostic tool giving different spectroscopic chiroptical signatures for all explored DNA sequences upon Cy7 binding. Cy7 was able to spectroscopically discriminate between the right handed B-DNA of poly(dG-dC)(2) and its left handed Z-DNA counterpart induced by spermine or Co(III)hexamine via nearly opposite induced circular dichroic signal.
format Online
Article
Text
id pubmed-3233455
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-32334552011-12-15 3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence Choi, Jung Kyu D’Urso, Alessandro Trauernicht, Mitch Shabbir-Hussain, Murtaza Holmes, Andrea E. Balaz, Milan Int J Mol Sci Article Using UV-vis absorption and circular dichroism (CD) spectroscopies, we explored the binding interactions of 3,3′-diethylthiatricarbocyanine iodide (Cy7) with polynucleotides of different sequences and helicity. CD showed to be a very diagnostic tool giving different spectroscopic chiroptical signatures for all explored DNA sequences upon Cy7 binding. Cy7 was able to spectroscopically discriminate between the right handed B-DNA of poly(dG-dC)(2) and its left handed Z-DNA counterpart induced by spermine or Co(III)hexamine via nearly opposite induced circular dichroic signal. Molecular Diversity Preservation International (MDPI) 2011-11-16 /pmc/articles/PMC3233455/ /pubmed/22174649 http://dx.doi.org/10.3390/ijms12118052 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Choi, Jung Kyu
D’Urso, Alessandro
Trauernicht, Mitch
Shabbir-Hussain, Murtaza
Holmes, Andrea E.
Balaz, Milan
3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence
title 3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence
title_full 3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence
title_fullStr 3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence
title_full_unstemmed 3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence
title_short 3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence
title_sort 3,3′-diethylthiatricarbocyanine iodide: a highly sensitive chiroptical reporter of dna helicity and sequence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233455/
https://www.ncbi.nlm.nih.gov/pubmed/22174649
http://dx.doi.org/10.3390/ijms12118052
work_keys_str_mv AT choijungkyu 33diethylthiatricarbocyanineiodideahighlysensitivechiropticalreporterofdnahelicityandsequence
AT dursoalessandro 33diethylthiatricarbocyanineiodideahighlysensitivechiropticalreporterofdnahelicityandsequence
AT trauernichtmitch 33diethylthiatricarbocyanineiodideahighlysensitivechiropticalreporterofdnahelicityandsequence
AT shabbirhussainmurtaza 33diethylthiatricarbocyanineiodideahighlysensitivechiropticalreporterofdnahelicityandsequence
AT holmesandreae 33diethylthiatricarbocyanineiodideahighlysensitivechiropticalreporterofdnahelicityandsequence
AT balazmilan 33diethylthiatricarbocyanineiodideahighlysensitivechiropticalreporterofdnahelicityandsequence

Ejemplares similares