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Dendrimer-Based Fluorescent Indicators: In Vitro and In Vivo Applications

BACKGROUND: The development of fluorescent proteins and synthetic molecules whose fluorescence properties are controlled by the environment makes it possible to monitor physiological and pathological events in living systems with minimal perturbation. A large number of small organic dyes are availab...

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Autores principales: Albertazzi, Lorenzo, Brondi, Marco, Pavan, Giovanni M., Sato, Sebastian Sulis, Signore, Giovanni, Storti, Barbara, Ratto, Gian Michele, Beltram, Fabio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233578/
https://www.ncbi.nlm.nih.gov/pubmed/22163303
http://dx.doi.org/10.1371/journal.pone.0028450
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author Albertazzi, Lorenzo
Brondi, Marco
Pavan, Giovanni M.
Sato, Sebastian Sulis
Signore, Giovanni
Storti, Barbara
Ratto, Gian Michele
Beltram, Fabio
author_facet Albertazzi, Lorenzo
Brondi, Marco
Pavan, Giovanni M.
Sato, Sebastian Sulis
Signore, Giovanni
Storti, Barbara
Ratto, Gian Michele
Beltram, Fabio
author_sort Albertazzi, Lorenzo
collection PubMed
description BACKGROUND: The development of fluorescent proteins and synthetic molecules whose fluorescence properties are controlled by the environment makes it possible to monitor physiological and pathological events in living systems with minimal perturbation. A large number of small organic dyes are available and routinely used to measure biologically relevant parameters. Unfortunately their application is hindered by a number of limitations stemming from the use of these small molecules in the biological environment. PRINCIPAL FINDINGS: We present a novel dendrimer-based architecture leading to multifunctional sensing elements that can overcome many of these problems. Applications in vitro, in living cells and in vivo are reported. In particular, we image for the first time extracellular pH in the brain in a mouse epilepsy model. CONCLUSION: We believe that the proposed architecture can represent a useful and novel tool in fluorescence imaging that can be widely applied in conjunction with a broad range of sensing dyes and experimental setups.
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spelling pubmed-32335782011-12-12 Dendrimer-Based Fluorescent Indicators: In Vitro and In Vivo Applications Albertazzi, Lorenzo Brondi, Marco Pavan, Giovanni M. Sato, Sebastian Sulis Signore, Giovanni Storti, Barbara Ratto, Gian Michele Beltram, Fabio PLoS One Research Article BACKGROUND: The development of fluorescent proteins and synthetic molecules whose fluorescence properties are controlled by the environment makes it possible to monitor physiological and pathological events in living systems with minimal perturbation. A large number of small organic dyes are available and routinely used to measure biologically relevant parameters. Unfortunately their application is hindered by a number of limitations stemming from the use of these small molecules in the biological environment. PRINCIPAL FINDINGS: We present a novel dendrimer-based architecture leading to multifunctional sensing elements that can overcome many of these problems. Applications in vitro, in living cells and in vivo are reported. In particular, we image for the first time extracellular pH in the brain in a mouse epilepsy model. CONCLUSION: We believe that the proposed architecture can represent a useful and novel tool in fluorescence imaging that can be widely applied in conjunction with a broad range of sensing dyes and experimental setups. Public Library of Science 2011-12-07 /pmc/articles/PMC3233578/ /pubmed/22163303 http://dx.doi.org/10.1371/journal.pone.0028450 Text en Albertazzi et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Albertazzi, Lorenzo
Brondi, Marco
Pavan, Giovanni M.
Sato, Sebastian Sulis
Signore, Giovanni
Storti, Barbara
Ratto, Gian Michele
Beltram, Fabio
Dendrimer-Based Fluorescent Indicators: In Vitro and In Vivo Applications
title Dendrimer-Based Fluorescent Indicators: In Vitro and In Vivo Applications
title_full Dendrimer-Based Fluorescent Indicators: In Vitro and In Vivo Applications
title_fullStr Dendrimer-Based Fluorescent Indicators: In Vitro and In Vivo Applications
title_full_unstemmed Dendrimer-Based Fluorescent Indicators: In Vitro and In Vivo Applications
title_short Dendrimer-Based Fluorescent Indicators: In Vitro and In Vivo Applications
title_sort dendrimer-based fluorescent indicators: in vitro and in vivo applications
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233578/
https://www.ncbi.nlm.nih.gov/pubmed/22163303
http://dx.doi.org/10.1371/journal.pone.0028450
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