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Galectin-1 Is Part of Human Trophoblast Invasion Machinery - A Functional Study In Vitro

BACKGROUND: Interactions of glycoconjugates with endogenous galectins, have been long proposed to participate in several reproductive processes including implantation. In human placenta gal-1, gal-3, gal-8, and gal-13 proteins are known to be present. Each of them has been proposed to play multiple...

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Autores principales: Kolundžić, Nikola, Bojić-Trbojević, Žanka, Kovačević, Tamara, Stefanoska, Ivana, Kadoya, Toshihiko, Vićovac, Ljiljana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3234277/
https://www.ncbi.nlm.nih.gov/pubmed/22174828
http://dx.doi.org/10.1371/journal.pone.0028514
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author Kolundžić, Nikola
Bojić-Trbojević, Žanka
Kovačević, Tamara
Stefanoska, Ivana
Kadoya, Toshihiko
Vićovac, Ljiljana
author_facet Kolundžić, Nikola
Bojić-Trbojević, Žanka
Kovačević, Tamara
Stefanoska, Ivana
Kadoya, Toshihiko
Vićovac, Ljiljana
author_sort Kolundžić, Nikola
collection PubMed
description BACKGROUND: Interactions of glycoconjugates with endogenous galectins, have been long proposed to participate in several reproductive processes including implantation. In human placenta gal-1, gal-3, gal-8, and gal-13 proteins are known to be present. Each of them has been proposed to play multiple functions, but so far no clear picture has emerged. We hypothesized that gal-1 participates in trophoblast invasion, and conducted Matrigel invasion assay using isolated cytotrophoblast from first trimester placenta and HTR-8/SVneo cell line to test it. METHODS AND FINDINGS: Function blocking anti-gal-1 antibody was employed to assess participation of endogenous gal-1 in cell adhesion, cell invasion of HTR-8/SVneo cells. When gal-1 was blocked in isolated trophoblast cell invasion was reduced to 75% of control (SEM±6.3, P<0.001) and to 66% of control (SEM±1.7, P<0.001) in HTR-8/SVneo cell line. Increased availability of gal-1, as two molecular forms of recombinant human gal-1 (CS-gal-1 and Ox-gal-1), resulted in increased cell invasion by cytotrophoblast to 151% (SEM±16, P<0.01) with 1 ng/ml of CS-gal-1, and to 192% (SEM±51, P<0.05) with 1 µg/ml of Ox-gal-1. Stimulation was also observed in HTR-8/SVneo cells, to 317% (SEM±58, P<0.001) by CS-gal-1, and to 200% (SEM±24, P<0.001) by Ox-gal-1 at 1 µg/ml. Both sets of results confirmed involvement of gal-1 in trophoblast invasion. Galectin profile of isolated cytotrophoblast and HTR-8/SVneo cells was established using RT-PCR and real-time PCR and found to consist of gal-1, gal-3 and gal-8 for both cell types. Only gal-1 was located at the trophoblast cell membrane, as determined by FACS analysis, which is consistent with the results of the functional tests. CONCLUSION AND SIGNIFICANCE: These findings qualify gal-1 as a member of human trophoblast cell invasion machinery.
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spelling pubmed-32342772011-12-15 Galectin-1 Is Part of Human Trophoblast Invasion Machinery - A Functional Study In Vitro Kolundžić, Nikola Bojić-Trbojević, Žanka Kovačević, Tamara Stefanoska, Ivana Kadoya, Toshihiko Vićovac, Ljiljana PLoS One Research Article BACKGROUND: Interactions of glycoconjugates with endogenous galectins, have been long proposed to participate in several reproductive processes including implantation. In human placenta gal-1, gal-3, gal-8, and gal-13 proteins are known to be present. Each of them has been proposed to play multiple functions, but so far no clear picture has emerged. We hypothesized that gal-1 participates in trophoblast invasion, and conducted Matrigel invasion assay using isolated cytotrophoblast from first trimester placenta and HTR-8/SVneo cell line to test it. METHODS AND FINDINGS: Function blocking anti-gal-1 antibody was employed to assess participation of endogenous gal-1 in cell adhesion, cell invasion of HTR-8/SVneo cells. When gal-1 was blocked in isolated trophoblast cell invasion was reduced to 75% of control (SEM±6.3, P<0.001) and to 66% of control (SEM±1.7, P<0.001) in HTR-8/SVneo cell line. Increased availability of gal-1, as two molecular forms of recombinant human gal-1 (CS-gal-1 and Ox-gal-1), resulted in increased cell invasion by cytotrophoblast to 151% (SEM±16, P<0.01) with 1 ng/ml of CS-gal-1, and to 192% (SEM±51, P<0.05) with 1 µg/ml of Ox-gal-1. Stimulation was also observed in HTR-8/SVneo cells, to 317% (SEM±58, P<0.001) by CS-gal-1, and to 200% (SEM±24, P<0.001) by Ox-gal-1 at 1 µg/ml. Both sets of results confirmed involvement of gal-1 in trophoblast invasion. Galectin profile of isolated cytotrophoblast and HTR-8/SVneo cells was established using RT-PCR and real-time PCR and found to consist of gal-1, gal-3 and gal-8 for both cell types. Only gal-1 was located at the trophoblast cell membrane, as determined by FACS analysis, which is consistent with the results of the functional tests. CONCLUSION AND SIGNIFICANCE: These findings qualify gal-1 as a member of human trophoblast cell invasion machinery. Public Library of Science 2011-12-08 /pmc/articles/PMC3234277/ /pubmed/22174828 http://dx.doi.org/10.1371/journal.pone.0028514 Text en Kolundžić et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kolundžić, Nikola
Bojić-Trbojević, Žanka
Kovačević, Tamara
Stefanoska, Ivana
Kadoya, Toshihiko
Vićovac, Ljiljana
Galectin-1 Is Part of Human Trophoblast Invasion Machinery - A Functional Study In Vitro
title Galectin-1 Is Part of Human Trophoblast Invasion Machinery - A Functional Study In Vitro
title_full Galectin-1 Is Part of Human Trophoblast Invasion Machinery - A Functional Study In Vitro
title_fullStr Galectin-1 Is Part of Human Trophoblast Invasion Machinery - A Functional Study In Vitro
title_full_unstemmed Galectin-1 Is Part of Human Trophoblast Invasion Machinery - A Functional Study In Vitro
title_short Galectin-1 Is Part of Human Trophoblast Invasion Machinery - A Functional Study In Vitro
title_sort galectin-1 is part of human trophoblast invasion machinery - a functional study in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3234277/
https://www.ncbi.nlm.nih.gov/pubmed/22174828
http://dx.doi.org/10.1371/journal.pone.0028514
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