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pcrEfficiency: a Web tool for PCR amplification efficiency prediction
BACKGROUND: Relative calculation of differential gene expression in quantitative PCR reactions requires comparison between amplification experiments that include reference genes and genes under study. Ignoring the differences between their efficiencies may lead to miscalculation of gene expression e...
Autores principales: | Mallona, Izaskun, Weiss, Julia, Marcos, Egea-Cortines |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3234296/ https://www.ncbi.nlm.nih.gov/pubmed/22014212 http://dx.doi.org/10.1186/1471-2105-12-404 |
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