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No Mutations of Lysophosphatidic Acid Receptor Genes in Lung Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in Rats
Lysophosphatidic acid (LPA) is a bioactive phospholipid that stimulates cell proliferation and migration, and protects cells from apoptosis. It interacts with specific G protein-coupled transmembrane receptors. Recently, frequent mutations of the LPA receptor-1 (LPA1) gene were detected in rat lung...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Toxicologic Pathology
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3234649/ https://www.ncbi.nlm.nih.gov/pubmed/22272013 http://dx.doi.org/10.1293/tox.23.63 |
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author | Wakabayashi, Naoko Tsujino, Megumu Tajiri, Masaki Taki, Midori Koshino, Ayumi Ikeda, Hiroko Fukushima, Nobuyuki Tsujiuchi, Toshifumi |
author_facet | Wakabayashi, Naoko Tsujino, Megumu Tajiri, Masaki Taki, Midori Koshino, Ayumi Ikeda, Hiroko Fukushima, Nobuyuki Tsujiuchi, Toshifumi |
author_sort | Wakabayashi, Naoko |
collection | PubMed |
description | Lysophosphatidic acid (LPA) is a bioactive phospholipid that stimulates cell proliferation and migration, and protects cells from apoptosis. It interacts with specific G protein-coupled transmembrane receptors. Recently, frequent mutations of the LPA receptor-1 (LPA1) gene were detected in rat lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine (BHP). In this study, to evaluate the involvement of other LPA receptor gene alterations during lung carcinogenesis, we investigated mutations of the LPA2, LPA3, LPA4 and LPA5 genes in lung adenocarcinomas induced by BHP in rats. Fifteen male Wistar rats, 6 weeks of age, were given 2000 ppm BHP in their drinking water for 12 weeks and then maintained without further treatment until sacrifice at 25 weeks, and 15 adenocarcinomas were obtained. Genomic DNAs were extracted from frozen tissues, and the LPA2, LPA3, LPA4 and LPA5 genes were examined for mutations, using polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) analysis. No mutations of LPA2, LPA3, LPA4 and LPA5 were detected in the 15 adenocarcinomas. These results suggest that alterations due to LPA2, LPA3, LPA4 and LPA5 gene mutations might not be involved in the development of lung adenocarcinomas induced by BHP in rats. |
format | Online Article Text |
id | pubmed-3234649 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The Japanese Society of Toxicologic Pathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-32346492012-01-23 No Mutations of Lysophosphatidic Acid Receptor Genes in Lung Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in Rats Wakabayashi, Naoko Tsujino, Megumu Tajiri, Masaki Taki, Midori Koshino, Ayumi Ikeda, Hiroko Fukushima, Nobuyuki Tsujiuchi, Toshifumi J Toxicol Pathol Short Communication Lysophosphatidic acid (LPA) is a bioactive phospholipid that stimulates cell proliferation and migration, and protects cells from apoptosis. It interacts with specific G protein-coupled transmembrane receptors. Recently, frequent mutations of the LPA receptor-1 (LPA1) gene were detected in rat lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine (BHP). In this study, to evaluate the involvement of other LPA receptor gene alterations during lung carcinogenesis, we investigated mutations of the LPA2, LPA3, LPA4 and LPA5 genes in lung adenocarcinomas induced by BHP in rats. Fifteen male Wistar rats, 6 weeks of age, were given 2000 ppm BHP in their drinking water for 12 weeks and then maintained without further treatment until sacrifice at 25 weeks, and 15 adenocarcinomas were obtained. Genomic DNAs were extracted from frozen tissues, and the LPA2, LPA3, LPA4 and LPA5 genes were examined for mutations, using polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) analysis. No mutations of LPA2, LPA3, LPA4 and LPA5 were detected in the 15 adenocarcinomas. These results suggest that alterations due to LPA2, LPA3, LPA4 and LPA5 gene mutations might not be involved in the development of lung adenocarcinomas induced by BHP in rats. The Japanese Society of Toxicologic Pathology 2010-03 2010-04-05 /pmc/articles/PMC3234649/ /pubmed/22272013 http://dx.doi.org/10.1293/tox.23.63 Text en 2010 The Japanese Society of Toxicologic Pathology http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Short Communication Wakabayashi, Naoko Tsujino, Megumu Tajiri, Masaki Taki, Midori Koshino, Ayumi Ikeda, Hiroko Fukushima, Nobuyuki Tsujiuchi, Toshifumi No Mutations of Lysophosphatidic Acid Receptor Genes in Lung Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in Rats |
title | No Mutations of Lysophosphatidic Acid Receptor Genes in Lung
Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in
Rats |
title_full | No Mutations of Lysophosphatidic Acid Receptor Genes in Lung
Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in
Rats |
title_fullStr | No Mutations of Lysophosphatidic Acid Receptor Genes in Lung
Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in
Rats |
title_full_unstemmed | No Mutations of Lysophosphatidic Acid Receptor Genes in Lung
Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in
Rats |
title_short | No Mutations of Lysophosphatidic Acid Receptor Genes in Lung
Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in
Rats |
title_sort | no mutations of lysophosphatidic acid receptor genes in lung
adenocarcinomas induced by n-nitrosobis(2-hydroxypropyl)amine in
rats |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3234649/ https://www.ncbi.nlm.nih.gov/pubmed/22272013 http://dx.doi.org/10.1293/tox.23.63 |
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