Cargando…

No Mutations of Lysophosphatidic Acid Receptor Genes in Lung Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in Rats

Lysophosphatidic acid (LPA) is a bioactive phospholipid that stimulates cell proliferation and migration, and protects cells from apoptosis. It interacts with specific G protein-coupled transmembrane receptors. Recently, frequent mutations of the LPA receptor-1 (LPA1) gene were detected in rat lung...

Descripción completa

Detalles Bibliográficos
Autores principales: Wakabayashi, Naoko, Tsujino, Megumu, Tajiri, Masaki, Taki, Midori, Koshino, Ayumi, Ikeda, Hiroko, Fukushima, Nobuyuki, Tsujiuchi, Toshifumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Toxicologic Pathology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3234649/
https://www.ncbi.nlm.nih.gov/pubmed/22272013
http://dx.doi.org/10.1293/tox.23.63
_version_ 1782218538800381952
author Wakabayashi, Naoko
Tsujino, Megumu
Tajiri, Masaki
Taki, Midori
Koshino, Ayumi
Ikeda, Hiroko
Fukushima, Nobuyuki
Tsujiuchi, Toshifumi
author_facet Wakabayashi, Naoko
Tsujino, Megumu
Tajiri, Masaki
Taki, Midori
Koshino, Ayumi
Ikeda, Hiroko
Fukushima, Nobuyuki
Tsujiuchi, Toshifumi
author_sort Wakabayashi, Naoko
collection PubMed
description Lysophosphatidic acid (LPA) is a bioactive phospholipid that stimulates cell proliferation and migration, and protects cells from apoptosis. It interacts with specific G protein-coupled transmembrane receptors. Recently, frequent mutations of the LPA receptor-1 (LPA1) gene were detected in rat lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine (BHP). In this study, to evaluate the involvement of other LPA receptor gene alterations during lung carcinogenesis, we investigated mutations of the LPA2, LPA3, LPA4 and LPA5 genes in lung adenocarcinomas induced by BHP in rats. Fifteen male Wistar rats, 6 weeks of age, were given 2000 ppm BHP in their drinking water for 12 weeks and then maintained without further treatment until sacrifice at 25 weeks, and 15 adenocarcinomas were obtained. Genomic DNAs were extracted from frozen tissues, and the LPA2, LPA3, LPA4 and LPA5 genes were examined for mutations, using polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) analysis. No mutations of LPA2, LPA3, LPA4 and LPA5 were detected in the 15 adenocarcinomas. These results suggest that alterations due to LPA2, LPA3, LPA4 and LPA5 gene mutations might not be involved in the development of lung adenocarcinomas induced by BHP in rats.
format Online
Article
Text
id pubmed-3234649
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher The Japanese Society of Toxicologic Pathology
record_format MEDLINE/PubMed
spelling pubmed-32346492012-01-23 No Mutations of Lysophosphatidic Acid Receptor Genes in Lung Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in Rats Wakabayashi, Naoko Tsujino, Megumu Tajiri, Masaki Taki, Midori Koshino, Ayumi Ikeda, Hiroko Fukushima, Nobuyuki Tsujiuchi, Toshifumi J Toxicol Pathol Short Communication Lysophosphatidic acid (LPA) is a bioactive phospholipid that stimulates cell proliferation and migration, and protects cells from apoptosis. It interacts with specific G protein-coupled transmembrane receptors. Recently, frequent mutations of the LPA receptor-1 (LPA1) gene were detected in rat lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine (BHP). In this study, to evaluate the involvement of other LPA receptor gene alterations during lung carcinogenesis, we investigated mutations of the LPA2, LPA3, LPA4 and LPA5 genes in lung adenocarcinomas induced by BHP in rats. Fifteen male Wistar rats, 6 weeks of age, were given 2000 ppm BHP in their drinking water for 12 weeks and then maintained without further treatment until sacrifice at 25 weeks, and 15 adenocarcinomas were obtained. Genomic DNAs were extracted from frozen tissues, and the LPA2, LPA3, LPA4 and LPA5 genes were examined for mutations, using polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) analysis. No mutations of LPA2, LPA3, LPA4 and LPA5 were detected in the 15 adenocarcinomas. These results suggest that alterations due to LPA2, LPA3, LPA4 and LPA5 gene mutations might not be involved in the development of lung adenocarcinomas induced by BHP in rats. The Japanese Society of Toxicologic Pathology 2010-03 2010-04-05 /pmc/articles/PMC3234649/ /pubmed/22272013 http://dx.doi.org/10.1293/tox.23.63 Text en 2010 The Japanese Society of Toxicologic Pathology http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Short Communication
Wakabayashi, Naoko
Tsujino, Megumu
Tajiri, Masaki
Taki, Midori
Koshino, Ayumi
Ikeda, Hiroko
Fukushima, Nobuyuki
Tsujiuchi, Toshifumi
No Mutations of Lysophosphatidic Acid Receptor Genes in Lung Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in Rats
title No Mutations of Lysophosphatidic Acid Receptor Genes in Lung Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in Rats
title_full No Mutations of Lysophosphatidic Acid Receptor Genes in Lung Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in Rats
title_fullStr No Mutations of Lysophosphatidic Acid Receptor Genes in Lung Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in Rats
title_full_unstemmed No Mutations of Lysophosphatidic Acid Receptor Genes in Lung Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in Rats
title_short No Mutations of Lysophosphatidic Acid Receptor Genes in Lung Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in Rats
title_sort no mutations of lysophosphatidic acid receptor genes in lung adenocarcinomas induced by n-nitrosobis(2-hydroxypropyl)amine in rats
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3234649/
https://www.ncbi.nlm.nih.gov/pubmed/22272013
http://dx.doi.org/10.1293/tox.23.63
work_keys_str_mv AT wakabayashinaoko nomutationsoflysophosphatidicacidreceptorgenesinlungadenocarcinomasinducedbynnitrosobis2hydroxypropylamineinrats
AT tsujinomegumu nomutationsoflysophosphatidicacidreceptorgenesinlungadenocarcinomasinducedbynnitrosobis2hydroxypropylamineinrats
AT tajirimasaki nomutationsoflysophosphatidicacidreceptorgenesinlungadenocarcinomasinducedbynnitrosobis2hydroxypropylamineinrats
AT takimidori nomutationsoflysophosphatidicacidreceptorgenesinlungadenocarcinomasinducedbynnitrosobis2hydroxypropylamineinrats
AT koshinoayumi nomutationsoflysophosphatidicacidreceptorgenesinlungadenocarcinomasinducedbynnitrosobis2hydroxypropylamineinrats
AT ikedahiroko nomutationsoflysophosphatidicacidreceptorgenesinlungadenocarcinomasinducedbynnitrosobis2hydroxypropylamineinrats
AT fukushimanobuyuki nomutationsoflysophosphatidicacidreceptorgenesinlungadenocarcinomasinducedbynnitrosobis2hydroxypropylamineinrats
AT tsujiuchitoshifumi nomutationsoflysophosphatidicacidreceptorgenesinlungadenocarcinomasinducedbynnitrosobis2hydroxypropylamineinrats