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Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines

BACKGROUND: A new simple preparation method for a hippurate-intercalated zinc-layered hydroxide (ZLH) nanohybrid has been established, which does not need an anion-exchange procedure to intercalate the hippurate anion into ZLH interlayers. METHODS: The hippuric acid nanohybrid (HAN) was prepared by...

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Autores principales: Ali, Samer Hasan Hussein Al, Al-Qubaisi, Mothanna, Hussein, Mohd Zobir, Zainal, Zulkarnain, Hakim, Muhammad Nazrul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3235030/
https://www.ncbi.nlm.nih.gov/pubmed/22163163
http://dx.doi.org/10.2147/IJN.S24510
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author Ali, Samer Hasan Hussein Al
Al-Qubaisi, Mothanna
Hussein, Mohd Zobir
Zainal, Zulkarnain
Hakim, Muhammad Nazrul
author_facet Ali, Samer Hasan Hussein Al
Al-Qubaisi, Mothanna
Hussein, Mohd Zobir
Zainal, Zulkarnain
Hakim, Muhammad Nazrul
author_sort Ali, Samer Hasan Hussein Al
collection PubMed
description BACKGROUND: A new simple preparation method for a hippurate-intercalated zinc-layered hydroxide (ZLH) nanohybrid has been established, which does not need an anion-exchange procedure to intercalate the hippurate anion into ZLH interlayers. METHODS: The hippuric acid nanohybrid (HAN) was prepared by direct reaction of an aqueous suspension of zinc oxide with a solution of hippuric acid via a one-step method. RESULTS: The basal spacing of the nanohybrid was 21.3 Å, indicating that the hippurate anion was successfully intercalated into the interlayer space of ZLH, and arranged in a monolayer fashion with the carboxylate group pointing toward the ZLH inorganic interlayers. A Fourier transform infrared study confirmed the formation of the nanohybrid, while thermogravimetry and differential thermogravimetry analyses showed that the thermal stability of the nanohybrid was markedly enhanced. The loading of hippurate in the nanohybrid was estimated to be about 38.7% (w/w), and the release of hippurate from the nanohybrid was of a controlled manner, and therefore the resulting material was suitable for use as a controlled-release formulation. HAN has synergistic properties with tamoxifen toward a HepG2 cell line, with an IC50 value of 0.35 compared with hippurate. In the antiproliferative assay, the ratio of viable cells account for cells treated by the combination tamoxifen with HAN to untreated cells was sharply reduced from 66% to 13% after 24 and 72 hours, respectively. CONCLUSION: The release of hippuric acid anions from HAN occurred in a controlled manner, and the resulting material is suitable for a controlled-release formulation.
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spelling pubmed-32350302011-12-12 Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines Ali, Samer Hasan Hussein Al Al-Qubaisi, Mothanna Hussein, Mohd Zobir Zainal, Zulkarnain Hakim, Muhammad Nazrul Int J Nanomedicine Original Research BACKGROUND: A new simple preparation method for a hippurate-intercalated zinc-layered hydroxide (ZLH) nanohybrid has been established, which does not need an anion-exchange procedure to intercalate the hippurate anion into ZLH interlayers. METHODS: The hippuric acid nanohybrid (HAN) was prepared by direct reaction of an aqueous suspension of zinc oxide with a solution of hippuric acid via a one-step method. RESULTS: The basal spacing of the nanohybrid was 21.3 Å, indicating that the hippurate anion was successfully intercalated into the interlayer space of ZLH, and arranged in a monolayer fashion with the carboxylate group pointing toward the ZLH inorganic interlayers. A Fourier transform infrared study confirmed the formation of the nanohybrid, while thermogravimetry and differential thermogravimetry analyses showed that the thermal stability of the nanohybrid was markedly enhanced. The loading of hippurate in the nanohybrid was estimated to be about 38.7% (w/w), and the release of hippurate from the nanohybrid was of a controlled manner, and therefore the resulting material was suitable for use as a controlled-release formulation. HAN has synergistic properties with tamoxifen toward a HepG2 cell line, with an IC50 value of 0.35 compared with hippurate. In the antiproliferative assay, the ratio of viable cells account for cells treated by the combination tamoxifen with HAN to untreated cells was sharply reduced from 66% to 13% after 24 and 72 hours, respectively. CONCLUSION: The release of hippuric acid anions from HAN occurred in a controlled manner, and the resulting material is suitable for a controlled-release formulation. Dove Medical Press 2011 2011-12-01 /pmc/articles/PMC3235030/ /pubmed/22163163 http://dx.doi.org/10.2147/IJN.S24510 Text en © 2011 Hussein Al Ali et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Ali, Samer Hasan Hussein Al
Al-Qubaisi, Mothanna
Hussein, Mohd Zobir
Zainal, Zulkarnain
Hakim, Muhammad Nazrul
Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines
title Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines
title_full Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines
title_fullStr Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines
title_full_unstemmed Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines
title_short Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines
title_sort preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on hepg2 cell lines
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3235030/
https://www.ncbi.nlm.nih.gov/pubmed/22163163
http://dx.doi.org/10.2147/IJN.S24510
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