Cargando…
Role of TRPM8 in dorsal root ganglion in nerve injury-induced chronic pain
BACKGROUND: Chronic neuropathic pain is an intractable pain with few effective treatments. Moderate cold stimulation can relieve pain, and this may be a novel train of thought for exploring new methods of analgesia. Transient receptor potential melastatin 8 (TRPM8) ion channel has been proposed to b...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3235975/ https://www.ncbi.nlm.nih.gov/pubmed/22111979 http://dx.doi.org/10.1186/1471-2202-12-120 |
_version_ | 1782218666961534976 |
---|---|
author | Su, Lin Wang, Chao Yu, Yong-hao Ren, Yong-ying Xie, Ke-liang Wang, Guo-lin |
author_facet | Su, Lin Wang, Chao Yu, Yong-hao Ren, Yong-ying Xie, Ke-liang Wang, Guo-lin |
author_sort | Su, Lin |
collection | PubMed |
description | BACKGROUND: Chronic neuropathic pain is an intractable pain with few effective treatments. Moderate cold stimulation can relieve pain, and this may be a novel train of thought for exploring new methods of analgesia. Transient receptor potential melastatin 8 (TRPM8) ion channel has been proposed to be an important molecular sensor for cold. Here we investigate the role of TRPM8 in the mechanism of chronic neuropathic pain using a rat model of chronic constriction injury (CCI) to the sciatic nerve. RESULTS: Mechanical allodynia, cold and thermal hyperalgesia of CCI rats began on the 4th day following surgery and maintained at the peak during the period from the 10th to 14th day after operation. The level of TRPM8 protein in L5 dorsal root ganglion (DRG) ipsilateral to nerve injury was significantly increased on the 4th day after CCI, and reached the peak on the 10th day, and remained elevated on the 14th day following CCI. This time course of the alteration of TRPM8 expression was consistent with that of CCI-induced hyperalgesic response of the operated hind paw. Besides, activation of cold receptor TRPM8 of CCI rats by intrathecal application of menthol resulted in the inhibition of mechanical allodynia and thermal hyperalgesia and the enhancement of cold hyperalgesia. In contrast, downregulation of TRPM8 protein in ipsilateral L5 DRG of CCI rats by intrathecal TRPM8 antisense oligonucleotide attenuated cold hyperalgesia, but it had no effect on CCI-induced mechanical allodynia and thermal hyperalgesia. CONCLUSIONS: TRPM8 may play different roles in mechanical allodynia, cold and thermal hyperalgesia that develop after nerve injury, and it is a very promising research direction for the development of new therapies for chronic neuroapthic pain. |
format | Online Article Text |
id | pubmed-3235975 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-32359752011-12-13 Role of TRPM8 in dorsal root ganglion in nerve injury-induced chronic pain Su, Lin Wang, Chao Yu, Yong-hao Ren, Yong-ying Xie, Ke-liang Wang, Guo-lin BMC Neurosci Research Article BACKGROUND: Chronic neuropathic pain is an intractable pain with few effective treatments. Moderate cold stimulation can relieve pain, and this may be a novel train of thought for exploring new methods of analgesia. Transient receptor potential melastatin 8 (TRPM8) ion channel has been proposed to be an important molecular sensor for cold. Here we investigate the role of TRPM8 in the mechanism of chronic neuropathic pain using a rat model of chronic constriction injury (CCI) to the sciatic nerve. RESULTS: Mechanical allodynia, cold and thermal hyperalgesia of CCI rats began on the 4th day following surgery and maintained at the peak during the period from the 10th to 14th day after operation. The level of TRPM8 protein in L5 dorsal root ganglion (DRG) ipsilateral to nerve injury was significantly increased on the 4th day after CCI, and reached the peak on the 10th day, and remained elevated on the 14th day following CCI. This time course of the alteration of TRPM8 expression was consistent with that of CCI-induced hyperalgesic response of the operated hind paw. Besides, activation of cold receptor TRPM8 of CCI rats by intrathecal application of menthol resulted in the inhibition of mechanical allodynia and thermal hyperalgesia and the enhancement of cold hyperalgesia. In contrast, downregulation of TRPM8 protein in ipsilateral L5 DRG of CCI rats by intrathecal TRPM8 antisense oligonucleotide attenuated cold hyperalgesia, but it had no effect on CCI-induced mechanical allodynia and thermal hyperalgesia. CONCLUSIONS: TRPM8 may play different roles in mechanical allodynia, cold and thermal hyperalgesia that develop after nerve injury, and it is a very promising research direction for the development of new therapies for chronic neuroapthic pain. BioMed Central 2011-11-23 /pmc/articles/PMC3235975/ /pubmed/22111979 http://dx.doi.org/10.1186/1471-2202-12-120 Text en Copyright ©2011 Su et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Su, Lin Wang, Chao Yu, Yong-hao Ren, Yong-ying Xie, Ke-liang Wang, Guo-lin Role of TRPM8 in dorsal root ganglion in nerve injury-induced chronic pain |
title | Role of TRPM8 in dorsal root ganglion in nerve injury-induced chronic pain |
title_full | Role of TRPM8 in dorsal root ganglion in nerve injury-induced chronic pain |
title_fullStr | Role of TRPM8 in dorsal root ganglion in nerve injury-induced chronic pain |
title_full_unstemmed | Role of TRPM8 in dorsal root ganglion in nerve injury-induced chronic pain |
title_short | Role of TRPM8 in dorsal root ganglion in nerve injury-induced chronic pain |
title_sort | role of trpm8 in dorsal root ganglion in nerve injury-induced chronic pain |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3235975/ https://www.ncbi.nlm.nih.gov/pubmed/22111979 http://dx.doi.org/10.1186/1471-2202-12-120 |
work_keys_str_mv | AT sulin roleoftrpm8indorsalrootganglioninnerveinjuryinducedchronicpain AT wangchao roleoftrpm8indorsalrootganglioninnerveinjuryinducedchronicpain AT yuyonghao roleoftrpm8indorsalrootganglioninnerveinjuryinducedchronicpain AT renyongying roleoftrpm8indorsalrootganglioninnerveinjuryinducedchronicpain AT xiekeliang roleoftrpm8indorsalrootganglioninnerveinjuryinducedchronicpain AT wangguolin roleoftrpm8indorsalrootganglioninnerveinjuryinducedchronicpain |