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Complete genome sequence of “Enterobacter lignolyticus” SCF1

In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated “Enterobacter lignolyticus” SCF1 on minimal media with alkali lignin as the sole source of carbon. This organism was isolated anaerobically from tropical forest soils collected from the Short Cloud Forest site in...

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Autores principales: DeAngelis, Kristen M., D’Haeseleer, Patrik, Chivian, Dylan, Fortney, Julian L., Khudyakov, Jane, Simmons, Blake, Woo, Hannah, Arkin, Adam P., Davenport, Karen Walston, Goodwin, Lynne, Chen, Amy, Ivanova, Natalia, Kyrpides, Nikos C., Mavromatis, Konstantinos, Woyke, Tanja, Hazen, Terry C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Michigan State University 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236048/
https://www.ncbi.nlm.nih.gov/pubmed/22180812
http://dx.doi.org/10.4056/sigs.2104875
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author DeAngelis, Kristen M.
D’Haeseleer, Patrik
Chivian, Dylan
Fortney, Julian L.
Khudyakov, Jane
Simmons, Blake
Woo, Hannah
Arkin, Adam P.
Davenport, Karen Walston
Goodwin, Lynne
Chen, Amy
Ivanova, Natalia
Kyrpides, Nikos C.
Mavromatis, Konstantinos
Woyke, Tanja
Hazen, Terry C.
author_facet DeAngelis, Kristen M.
D’Haeseleer, Patrik
Chivian, Dylan
Fortney, Julian L.
Khudyakov, Jane
Simmons, Blake
Woo, Hannah
Arkin, Adam P.
Davenport, Karen Walston
Goodwin, Lynne
Chen, Amy
Ivanova, Natalia
Kyrpides, Nikos C.
Mavromatis, Konstantinos
Woyke, Tanja
Hazen, Terry C.
author_sort DeAngelis, Kristen M.
collection PubMed
description In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated “Enterobacter lignolyticus” SCF1 on minimal media with alkali lignin as the sole source of carbon. This organism was isolated anaerobically from tropical forest soils collected from the Short Cloud Forest site in the El Yunque National Forest in Puerto Rico, USA, part of the Luquillo Long-Term Ecological Research Station. At this site, the soils experience strong fluctuations in redox potential and are net methane producers. Because of its ability to grow on lignin anaerobically, we sequenced the genome. The genome of “E. lignolyticus” SCF1 is 4.81 Mbp with no detected plasmids, and includes a relatively small arsenal of lignocellulolytic carbohydrate active enzymes. Lignin degradation was observed in culture, and the genome revealed two putative laccases, a putative peroxidase, and a complete 4-hydroxyphenylacetate degradation pathway encoded in a single gene cluster.
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spelling pubmed-32360482011-12-16 Complete genome sequence of “Enterobacter lignolyticus” SCF1 DeAngelis, Kristen M. D’Haeseleer, Patrik Chivian, Dylan Fortney, Julian L. Khudyakov, Jane Simmons, Blake Woo, Hannah Arkin, Adam P. Davenport, Karen Walston Goodwin, Lynne Chen, Amy Ivanova, Natalia Kyrpides, Nikos C. Mavromatis, Konstantinos Woyke, Tanja Hazen, Terry C. Stand Genomic Sci Short Genome Reports In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated “Enterobacter lignolyticus” SCF1 on minimal media with alkali lignin as the sole source of carbon. This organism was isolated anaerobically from tropical forest soils collected from the Short Cloud Forest site in the El Yunque National Forest in Puerto Rico, USA, part of the Luquillo Long-Term Ecological Research Station. At this site, the soils experience strong fluctuations in redox potential and are net methane producers. Because of its ability to grow on lignin anaerobically, we sequenced the genome. The genome of “E. lignolyticus” SCF1 is 4.81 Mbp with no detected plasmids, and includes a relatively small arsenal of lignocellulolytic carbohydrate active enzymes. Lignin degradation was observed in culture, and the genome revealed two putative laccases, a putative peroxidase, and a complete 4-hydroxyphenylacetate degradation pathway encoded in a single gene cluster. Michigan State University 2011-09-23 /pmc/articles/PMC3236048/ /pubmed/22180812 http://dx.doi.org/10.4056/sigs.2104875 Text en http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Genome Reports
DeAngelis, Kristen M.
D’Haeseleer, Patrik
Chivian, Dylan
Fortney, Julian L.
Khudyakov, Jane
Simmons, Blake
Woo, Hannah
Arkin, Adam P.
Davenport, Karen Walston
Goodwin, Lynne
Chen, Amy
Ivanova, Natalia
Kyrpides, Nikos C.
Mavromatis, Konstantinos
Woyke, Tanja
Hazen, Terry C.
Complete genome sequence of “Enterobacter lignolyticus” SCF1
title Complete genome sequence of “Enterobacter lignolyticus” SCF1
title_full Complete genome sequence of “Enterobacter lignolyticus” SCF1
title_fullStr Complete genome sequence of “Enterobacter lignolyticus” SCF1
title_full_unstemmed Complete genome sequence of “Enterobacter lignolyticus” SCF1
title_short Complete genome sequence of “Enterobacter lignolyticus” SCF1
title_sort complete genome sequence of “enterobacter lignolyticus” scf1
topic Short Genome Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236048/
https://www.ncbi.nlm.nih.gov/pubmed/22180812
http://dx.doi.org/10.4056/sigs.2104875
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