Cargando…
Complete genome sequence of “Enterobacter lignolyticus” SCF1
In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated “Enterobacter lignolyticus” SCF1 on minimal media with alkali lignin as the sole source of carbon. This organism was isolated anaerobically from tropical forest soils collected from the Short Cloud Forest site in...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Michigan State University
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236048/ https://www.ncbi.nlm.nih.gov/pubmed/22180812 http://dx.doi.org/10.4056/sigs.2104875 |
_version_ | 1782218680227069952 |
---|---|
author | DeAngelis, Kristen M. D’Haeseleer, Patrik Chivian, Dylan Fortney, Julian L. Khudyakov, Jane Simmons, Blake Woo, Hannah Arkin, Adam P. Davenport, Karen Walston Goodwin, Lynne Chen, Amy Ivanova, Natalia Kyrpides, Nikos C. Mavromatis, Konstantinos Woyke, Tanja Hazen, Terry C. |
author_facet | DeAngelis, Kristen M. D’Haeseleer, Patrik Chivian, Dylan Fortney, Julian L. Khudyakov, Jane Simmons, Blake Woo, Hannah Arkin, Adam P. Davenport, Karen Walston Goodwin, Lynne Chen, Amy Ivanova, Natalia Kyrpides, Nikos C. Mavromatis, Konstantinos Woyke, Tanja Hazen, Terry C. |
author_sort | DeAngelis, Kristen M. |
collection | PubMed |
description | In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated “Enterobacter lignolyticus” SCF1 on minimal media with alkali lignin as the sole source of carbon. This organism was isolated anaerobically from tropical forest soils collected from the Short Cloud Forest site in the El Yunque National Forest in Puerto Rico, USA, part of the Luquillo Long-Term Ecological Research Station. At this site, the soils experience strong fluctuations in redox potential and are net methane producers. Because of its ability to grow on lignin anaerobically, we sequenced the genome. The genome of “E. lignolyticus” SCF1 is 4.81 Mbp with no detected plasmids, and includes a relatively small arsenal of lignocellulolytic carbohydrate active enzymes. Lignin degradation was observed in culture, and the genome revealed two putative laccases, a putative peroxidase, and a complete 4-hydroxyphenylacetate degradation pathway encoded in a single gene cluster. |
format | Online Article Text |
id | pubmed-3236048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Michigan State University |
record_format | MEDLINE/PubMed |
spelling | pubmed-32360482011-12-16 Complete genome sequence of “Enterobacter lignolyticus” SCF1 DeAngelis, Kristen M. D’Haeseleer, Patrik Chivian, Dylan Fortney, Julian L. Khudyakov, Jane Simmons, Blake Woo, Hannah Arkin, Adam P. Davenport, Karen Walston Goodwin, Lynne Chen, Amy Ivanova, Natalia Kyrpides, Nikos C. Mavromatis, Konstantinos Woyke, Tanja Hazen, Terry C. Stand Genomic Sci Short Genome Reports In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated “Enterobacter lignolyticus” SCF1 on minimal media with alkali lignin as the sole source of carbon. This organism was isolated anaerobically from tropical forest soils collected from the Short Cloud Forest site in the El Yunque National Forest in Puerto Rico, USA, part of the Luquillo Long-Term Ecological Research Station. At this site, the soils experience strong fluctuations in redox potential and are net methane producers. Because of its ability to grow on lignin anaerobically, we sequenced the genome. The genome of “E. lignolyticus” SCF1 is 4.81 Mbp with no detected plasmids, and includes a relatively small arsenal of lignocellulolytic carbohydrate active enzymes. Lignin degradation was observed in culture, and the genome revealed two putative laccases, a putative peroxidase, and a complete 4-hydroxyphenylacetate degradation pathway encoded in a single gene cluster. Michigan State University 2011-09-23 /pmc/articles/PMC3236048/ /pubmed/22180812 http://dx.doi.org/10.4056/sigs.2104875 Text en http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Genome Reports DeAngelis, Kristen M. D’Haeseleer, Patrik Chivian, Dylan Fortney, Julian L. Khudyakov, Jane Simmons, Blake Woo, Hannah Arkin, Adam P. Davenport, Karen Walston Goodwin, Lynne Chen, Amy Ivanova, Natalia Kyrpides, Nikos C. Mavromatis, Konstantinos Woyke, Tanja Hazen, Terry C. Complete genome sequence of “Enterobacter lignolyticus” SCF1 |
title | Complete genome sequence of “Enterobacter lignolyticus” SCF1 |
title_full | Complete genome sequence of “Enterobacter lignolyticus” SCF1 |
title_fullStr | Complete genome sequence of “Enterobacter lignolyticus” SCF1 |
title_full_unstemmed | Complete genome sequence of “Enterobacter lignolyticus” SCF1 |
title_short | Complete genome sequence of “Enterobacter lignolyticus” SCF1 |
title_sort | complete genome sequence of “enterobacter lignolyticus” scf1 |
topic | Short Genome Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236048/ https://www.ncbi.nlm.nih.gov/pubmed/22180812 http://dx.doi.org/10.4056/sigs.2104875 |
work_keys_str_mv | AT deangeliskristenm completegenomesequenceofenterobacterlignolyticusscf1 AT dhaeseleerpatrik completegenomesequenceofenterobacterlignolyticusscf1 AT chiviandylan completegenomesequenceofenterobacterlignolyticusscf1 AT fortneyjulianl completegenomesequenceofenterobacterlignolyticusscf1 AT khudyakovjane completegenomesequenceofenterobacterlignolyticusscf1 AT simmonsblake completegenomesequenceofenterobacterlignolyticusscf1 AT woohannah completegenomesequenceofenterobacterlignolyticusscf1 AT arkinadamp completegenomesequenceofenterobacterlignolyticusscf1 AT davenportkarenwalston completegenomesequenceofenterobacterlignolyticusscf1 AT goodwinlynne completegenomesequenceofenterobacterlignolyticusscf1 AT chenamy completegenomesequenceofenterobacterlignolyticusscf1 AT ivanovanatalia completegenomesequenceofenterobacterlignolyticusscf1 AT kyrpidesnikosc completegenomesequenceofenterobacterlignolyticusscf1 AT mavromatiskonstantinos completegenomesequenceofenterobacterlignolyticusscf1 AT woyketanja completegenomesequenceofenterobacterlignolyticusscf1 AT hazenterryc completegenomesequenceofenterobacterlignolyticusscf1 |