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Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes

Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in signaling and defense that orchestrate and execute the response i...

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Autores principales: Bazzini, Ariel A., Manacorda, Carlos A., Tohge, Takayuki, Conti, Gabriela, Rodriguez, Maria C., Nunes-Nesi, Adriano, Villanueva, Sofía, Fernie, Alisdair R., Carrari, Fernando, Asurmendi, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236191/
https://www.ncbi.nlm.nih.gov/pubmed/22174812
http://dx.doi.org/10.1371/journal.pone.0028466
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author Bazzini, Ariel A.
Manacorda, Carlos A.
Tohge, Takayuki
Conti, Gabriela
Rodriguez, Maria C.
Nunes-Nesi, Adriano
Villanueva, Sofía
Fernie, Alisdair R.
Carrari, Fernando
Asurmendi, Sebastian
author_facet Bazzini, Ariel A.
Manacorda, Carlos A.
Tohge, Takayuki
Conti, Gabriela
Rodriguez, Maria C.
Nunes-Nesi, Adriano
Villanueva, Sofía
Fernie, Alisdair R.
Carrari, Fernando
Asurmendi, Sebastian
author_sort Bazzini, Ariel A.
collection PubMed
description Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the relative level of metabolites across the viral infection, together with a miRs profiling using a micro-array based procedure. Systemic changes in metabolites were characterized by a biphasic response after infection. The first phase, detected at one dpi, evidenced the action of a systemic signal since no virus was detected systemically. Several of the metabolites increased at this stage were hormone-related. miRs profiling after infection also revealed a biphasic alteration, showing miRs alteration at 5 dpi where no virus was detected systemically and a late phase correlating with virus accumulation. Correlation analyses revealed a massive increase in the density of correlation networks after infection indicating a complex reprogramming of the regulatory pathways, either in response to the plant defense mechanism or to the virus infection itself. Our data propose the involvement of a systemic signaling on early miRs alteration.
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spelling pubmed-32361912011-12-15 Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes Bazzini, Ariel A. Manacorda, Carlos A. Tohge, Takayuki Conti, Gabriela Rodriguez, Maria C. Nunes-Nesi, Adriano Villanueva, Sofía Fernie, Alisdair R. Carrari, Fernando Asurmendi, Sebastian PLoS One Research Article Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the relative level of metabolites across the viral infection, together with a miRs profiling using a micro-array based procedure. Systemic changes in metabolites were characterized by a biphasic response after infection. The first phase, detected at one dpi, evidenced the action of a systemic signal since no virus was detected systemically. Several of the metabolites increased at this stage were hormone-related. miRs profiling after infection also revealed a biphasic alteration, showing miRs alteration at 5 dpi where no virus was detected systemically and a late phase correlating with virus accumulation. Correlation analyses revealed a massive increase in the density of correlation networks after infection indicating a complex reprogramming of the regulatory pathways, either in response to the plant defense mechanism or to the virus infection itself. Our data propose the involvement of a systemic signaling on early miRs alteration. Public Library of Science 2011-12-12 /pmc/articles/PMC3236191/ /pubmed/22174812 http://dx.doi.org/10.1371/journal.pone.0028466 Text en Bazzini et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bazzini, Ariel A.
Manacorda, Carlos A.
Tohge, Takayuki
Conti, Gabriela
Rodriguez, Maria C.
Nunes-Nesi, Adriano
Villanueva, Sofía
Fernie, Alisdair R.
Carrari, Fernando
Asurmendi, Sebastian
Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes
title Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes
title_full Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes
title_fullStr Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes
title_full_unstemmed Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes
title_short Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes
title_sort metabolic and mirna profiling of tmv infected plants reveals biphasic temporal changes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236191/
https://www.ncbi.nlm.nih.gov/pubmed/22174812
http://dx.doi.org/10.1371/journal.pone.0028466
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