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Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance

Hydrogen peroxide (H(2)O(2)) plays important roles in plant biotic and abiotic stress responses. However, the effect of H(2)O(2) stress on the bread wheat transcriptome is still lacking. To investigate the cellular and metabolic responses triggered by H(2)O(2), we performed an mRNA tag analysis of w...

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Autores principales: Li, Aili, Zhang, Rongzhi, Pan, Lei, Tang, Lichuan, Zhao, Guangyao, Zhu, Mingzhu, Chu, Jinfang, Sun, Xiaohong, Wei, Bo, Zhang, Xiangqi, Jia, Jizeng, Mao, Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236209/
https://www.ncbi.nlm.nih.gov/pubmed/22174904
http://dx.doi.org/10.1371/journal.pone.0028810
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author Li, Aili
Zhang, Rongzhi
Pan, Lei
Tang, Lichuan
Zhao, Guangyao
Zhu, Mingzhu
Chu, Jinfang
Sun, Xiaohong
Wei, Bo
Zhang, Xiangqi
Jia, Jizeng
Mao, Long
author_facet Li, Aili
Zhang, Rongzhi
Pan, Lei
Tang, Lichuan
Zhao, Guangyao
Zhu, Mingzhu
Chu, Jinfang
Sun, Xiaohong
Wei, Bo
Zhang, Xiangqi
Jia, Jizeng
Mao, Long
author_sort Li, Aili
collection PubMed
description Hydrogen peroxide (H(2)O(2)) plays important roles in plant biotic and abiotic stress responses. However, the effect of H(2)O(2) stress on the bread wheat transcriptome is still lacking. To investigate the cellular and metabolic responses triggered by H(2)O(2), we performed an mRNA tag analysis of wheat seedlings under 10 mM H(2)O(2) treatment for 6 hour in one powdery mildew (PM) resistant (PmA) and two susceptible (Cha and Han) lines. In total, 6,156, 6,875 and 3,276 transcripts were found to be differentially expressed in PmA, Han and Cha respectively. Among them, 260 genes exhibited consistent expression patterns in all three wheat lines and may represent a subset of basal H(2)O(2) responsive genes that were associated with cell defense, signal transduction, photosynthesis, carbohydrate metabolism, lipid metabolism, redox homeostasis, and transport. Among genes specific to PmA, ‘transport’ activity was significantly enriched in Gene Ontology analysis. MapMan classification showed that, while both up- and down- regulations were observed for auxin, abscisic acid, and brassinolides signaling genes, the jasmonic acid and ethylene signaling pathway genes were all up-regulated, suggesting H(2)O(2)-enhanced JA/Et functions in PmA. To further study whether any of these genes were involved in wheat PM response, 19 H(2)O(2)-responsive putative defense related genes were assayed in wheat seedlings infected with Blumeria graminis f. sp. tritici (Bgt). Eight of these genes were found to be co-regulated by H(2)O(2) and Bgt, among which a fatty acid desaturase gene TaFAD was then confirmed by virus induced gene silencing (VIGS) to be required for the PM resistance. Together, our data presents the first global picture of the wheat transcriptome under H(2)O(2) stress and uncovers potential links between H(2)O(2) and Bgt responses, hence providing important candidate genes for the PM resistance in wheat.
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spelling pubmed-32362092011-12-15 Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance Li, Aili Zhang, Rongzhi Pan, Lei Tang, Lichuan Zhao, Guangyao Zhu, Mingzhu Chu, Jinfang Sun, Xiaohong Wei, Bo Zhang, Xiangqi Jia, Jizeng Mao, Long PLoS One Research Article Hydrogen peroxide (H(2)O(2)) plays important roles in plant biotic and abiotic stress responses. However, the effect of H(2)O(2) stress on the bread wheat transcriptome is still lacking. To investigate the cellular and metabolic responses triggered by H(2)O(2), we performed an mRNA tag analysis of wheat seedlings under 10 mM H(2)O(2) treatment for 6 hour in one powdery mildew (PM) resistant (PmA) and two susceptible (Cha and Han) lines. In total, 6,156, 6,875 and 3,276 transcripts were found to be differentially expressed in PmA, Han and Cha respectively. Among them, 260 genes exhibited consistent expression patterns in all three wheat lines and may represent a subset of basal H(2)O(2) responsive genes that were associated with cell defense, signal transduction, photosynthesis, carbohydrate metabolism, lipid metabolism, redox homeostasis, and transport. Among genes specific to PmA, ‘transport’ activity was significantly enriched in Gene Ontology analysis. MapMan classification showed that, while both up- and down- regulations were observed for auxin, abscisic acid, and brassinolides signaling genes, the jasmonic acid and ethylene signaling pathway genes were all up-regulated, suggesting H(2)O(2)-enhanced JA/Et functions in PmA. To further study whether any of these genes were involved in wheat PM response, 19 H(2)O(2)-responsive putative defense related genes were assayed in wheat seedlings infected with Blumeria graminis f. sp. tritici (Bgt). Eight of these genes were found to be co-regulated by H(2)O(2) and Bgt, among which a fatty acid desaturase gene TaFAD was then confirmed by virus induced gene silencing (VIGS) to be required for the PM resistance. Together, our data presents the first global picture of the wheat transcriptome under H(2)O(2) stress and uncovers potential links between H(2)O(2) and Bgt responses, hence providing important candidate genes for the PM resistance in wheat. Public Library of Science 2011-12-12 /pmc/articles/PMC3236209/ /pubmed/22174904 http://dx.doi.org/10.1371/journal.pone.0028810 Text en Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Li, Aili
Zhang, Rongzhi
Pan, Lei
Tang, Lichuan
Zhao, Guangyao
Zhu, Mingzhu
Chu, Jinfang
Sun, Xiaohong
Wei, Bo
Zhang, Xiangqi
Jia, Jizeng
Mao, Long
Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance
title Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance
title_full Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance
title_fullStr Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance
title_full_unstemmed Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance
title_short Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance
title_sort transcriptome analysis of h(2)o(2)-treated wheat seedlings reveals a h(2)o(2)-responsive fatty acid desaturase gene participating in powdery mildew resistance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236209/
https://www.ncbi.nlm.nih.gov/pubmed/22174904
http://dx.doi.org/10.1371/journal.pone.0028810
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