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Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance
Hydrogen peroxide (H(2)O(2)) plays important roles in plant biotic and abiotic stress responses. However, the effect of H(2)O(2) stress on the bread wheat transcriptome is still lacking. To investigate the cellular and metabolic responses triggered by H(2)O(2), we performed an mRNA tag analysis of w...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236209/ https://www.ncbi.nlm.nih.gov/pubmed/22174904 http://dx.doi.org/10.1371/journal.pone.0028810 |
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author | Li, Aili Zhang, Rongzhi Pan, Lei Tang, Lichuan Zhao, Guangyao Zhu, Mingzhu Chu, Jinfang Sun, Xiaohong Wei, Bo Zhang, Xiangqi Jia, Jizeng Mao, Long |
author_facet | Li, Aili Zhang, Rongzhi Pan, Lei Tang, Lichuan Zhao, Guangyao Zhu, Mingzhu Chu, Jinfang Sun, Xiaohong Wei, Bo Zhang, Xiangqi Jia, Jizeng Mao, Long |
author_sort | Li, Aili |
collection | PubMed |
description | Hydrogen peroxide (H(2)O(2)) plays important roles in plant biotic and abiotic stress responses. However, the effect of H(2)O(2) stress on the bread wheat transcriptome is still lacking. To investigate the cellular and metabolic responses triggered by H(2)O(2), we performed an mRNA tag analysis of wheat seedlings under 10 mM H(2)O(2) treatment for 6 hour in one powdery mildew (PM) resistant (PmA) and two susceptible (Cha and Han) lines. In total, 6,156, 6,875 and 3,276 transcripts were found to be differentially expressed in PmA, Han and Cha respectively. Among them, 260 genes exhibited consistent expression patterns in all three wheat lines and may represent a subset of basal H(2)O(2) responsive genes that were associated with cell defense, signal transduction, photosynthesis, carbohydrate metabolism, lipid metabolism, redox homeostasis, and transport. Among genes specific to PmA, ‘transport’ activity was significantly enriched in Gene Ontology analysis. MapMan classification showed that, while both up- and down- regulations were observed for auxin, abscisic acid, and brassinolides signaling genes, the jasmonic acid and ethylene signaling pathway genes were all up-regulated, suggesting H(2)O(2)-enhanced JA/Et functions in PmA. To further study whether any of these genes were involved in wheat PM response, 19 H(2)O(2)-responsive putative defense related genes were assayed in wheat seedlings infected with Blumeria graminis f. sp. tritici (Bgt). Eight of these genes were found to be co-regulated by H(2)O(2) and Bgt, among which a fatty acid desaturase gene TaFAD was then confirmed by virus induced gene silencing (VIGS) to be required for the PM resistance. Together, our data presents the first global picture of the wheat transcriptome under H(2)O(2) stress and uncovers potential links between H(2)O(2) and Bgt responses, hence providing important candidate genes for the PM resistance in wheat. |
format | Online Article Text |
id | pubmed-3236209 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32362092011-12-15 Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance Li, Aili Zhang, Rongzhi Pan, Lei Tang, Lichuan Zhao, Guangyao Zhu, Mingzhu Chu, Jinfang Sun, Xiaohong Wei, Bo Zhang, Xiangqi Jia, Jizeng Mao, Long PLoS One Research Article Hydrogen peroxide (H(2)O(2)) plays important roles in plant biotic and abiotic stress responses. However, the effect of H(2)O(2) stress on the bread wheat transcriptome is still lacking. To investigate the cellular and metabolic responses triggered by H(2)O(2), we performed an mRNA tag analysis of wheat seedlings under 10 mM H(2)O(2) treatment for 6 hour in one powdery mildew (PM) resistant (PmA) and two susceptible (Cha and Han) lines. In total, 6,156, 6,875 and 3,276 transcripts were found to be differentially expressed in PmA, Han and Cha respectively. Among them, 260 genes exhibited consistent expression patterns in all three wheat lines and may represent a subset of basal H(2)O(2) responsive genes that were associated with cell defense, signal transduction, photosynthesis, carbohydrate metabolism, lipid metabolism, redox homeostasis, and transport. Among genes specific to PmA, ‘transport’ activity was significantly enriched in Gene Ontology analysis. MapMan classification showed that, while both up- and down- regulations were observed for auxin, abscisic acid, and brassinolides signaling genes, the jasmonic acid and ethylene signaling pathway genes were all up-regulated, suggesting H(2)O(2)-enhanced JA/Et functions in PmA. To further study whether any of these genes were involved in wheat PM response, 19 H(2)O(2)-responsive putative defense related genes were assayed in wheat seedlings infected with Blumeria graminis f. sp. tritici (Bgt). Eight of these genes were found to be co-regulated by H(2)O(2) and Bgt, among which a fatty acid desaturase gene TaFAD was then confirmed by virus induced gene silencing (VIGS) to be required for the PM resistance. Together, our data presents the first global picture of the wheat transcriptome under H(2)O(2) stress and uncovers potential links between H(2)O(2) and Bgt responses, hence providing important candidate genes for the PM resistance in wheat. Public Library of Science 2011-12-12 /pmc/articles/PMC3236209/ /pubmed/22174904 http://dx.doi.org/10.1371/journal.pone.0028810 Text en Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Li, Aili Zhang, Rongzhi Pan, Lei Tang, Lichuan Zhao, Guangyao Zhu, Mingzhu Chu, Jinfang Sun, Xiaohong Wei, Bo Zhang, Xiangqi Jia, Jizeng Mao, Long Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance |
title | Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance |
title_full | Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance |
title_fullStr | Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance |
title_full_unstemmed | Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance |
title_short | Transcriptome Analysis of H(2)O(2)-Treated Wheat Seedlings Reveals a H(2)O(2)-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance |
title_sort | transcriptome analysis of h(2)o(2)-treated wheat seedlings reveals a h(2)o(2)-responsive fatty acid desaturase gene participating in powdery mildew resistance |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236209/ https://www.ncbi.nlm.nih.gov/pubmed/22174904 http://dx.doi.org/10.1371/journal.pone.0028810 |
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