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Size-dependent endocytosis of gold nanoparticles studied by three-dimensional mapping of plasmonic scattering images
BACKGROUND: Understanding the endocytosis process of gold nanoparticles (AuNPs) is important for the drug delivery and photodynamic therapy applications. The endocytosis in living cells is usually studied by fluorescent microscopy. The fluorescent labeling suffers from photobleaching. Besides, quant...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236302/ https://www.ncbi.nlm.nih.gov/pubmed/21167077 http://dx.doi.org/10.1186/1477-3155-8-33 |
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author | Wang, Sheng-Hann Lee, Chia-Wei Chiou, Arthur Wei, Pei-Kuen |
author_facet | Wang, Sheng-Hann Lee, Chia-Wei Chiou, Arthur Wei, Pei-Kuen |
author_sort | Wang, Sheng-Hann |
collection | PubMed |
description | BACKGROUND: Understanding the endocytosis process of gold nanoparticles (AuNPs) is important for the drug delivery and photodynamic therapy applications. The endocytosis in living cells is usually studied by fluorescent microscopy. The fluorescent labeling suffers from photobleaching. Besides, quantitative estimation of the cellular uptake is not easy. In this paper, the size-dependent endocytosis of AuNPs was investigated by using plasmonic scattering images without any labeling. RESULTS: The scattering images of AuNPs and the vesicles were mapped by using an optical sectioning microscopy with dark-field illumination. AuNPs have large optical scatterings at 550-600 nm wavelengths due to localized surface plasmon resonances. Using an enhanced contrast between yellow and blue CCD images, AuNPs can be well distinguished from cellular organelles. The tracking of AuNPs coated with aptamers for surface mucin glycoprotein shows that AuNPs attached to extracellular matrix and moved towards center of the cell. Most 75-nm-AuNPs moved to the top of cells, while many 45-nm-AuNPs entered cells through endocytosis and accumulated in endocytic vesicles. The amounts of cellular uptake decreased with the increase of particle size. CONCLUSIONS: We quantitatively studied the endocytosis of AuNPs with different sizes in various cancer cells. The plasmonic scattering images confirm the size-dependent endocytosis of AuNPs. The 45-nm-AuNP is better for drug delivery due to its higher uptake rate. On the other hand, large AuNPs are immobilized on the cell membrane. They can be used to reconstruct the cell morphology. |
format | Online Article Text |
id | pubmed-3236302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-32363022011-12-14 Size-dependent endocytosis of gold nanoparticles studied by three-dimensional mapping of plasmonic scattering images Wang, Sheng-Hann Lee, Chia-Wei Chiou, Arthur Wei, Pei-Kuen J Nanobiotechnology Research BACKGROUND: Understanding the endocytosis process of gold nanoparticles (AuNPs) is important for the drug delivery and photodynamic therapy applications. The endocytosis in living cells is usually studied by fluorescent microscopy. The fluorescent labeling suffers from photobleaching. Besides, quantitative estimation of the cellular uptake is not easy. In this paper, the size-dependent endocytosis of AuNPs was investigated by using plasmonic scattering images without any labeling. RESULTS: The scattering images of AuNPs and the vesicles were mapped by using an optical sectioning microscopy with dark-field illumination. AuNPs have large optical scatterings at 550-600 nm wavelengths due to localized surface plasmon resonances. Using an enhanced contrast between yellow and blue CCD images, AuNPs can be well distinguished from cellular organelles. The tracking of AuNPs coated with aptamers for surface mucin glycoprotein shows that AuNPs attached to extracellular matrix and moved towards center of the cell. Most 75-nm-AuNPs moved to the top of cells, while many 45-nm-AuNPs entered cells through endocytosis and accumulated in endocytic vesicles. The amounts of cellular uptake decreased with the increase of particle size. CONCLUSIONS: We quantitatively studied the endocytosis of AuNPs with different sizes in various cancer cells. The plasmonic scattering images confirm the size-dependent endocytosis of AuNPs. The 45-nm-AuNP is better for drug delivery due to its higher uptake rate. On the other hand, large AuNPs are immobilized on the cell membrane. They can be used to reconstruct the cell morphology. BioMed Central 2010-12-20 /pmc/articles/PMC3236302/ /pubmed/21167077 http://dx.doi.org/10.1186/1477-3155-8-33 Text en Copyright © 2010 Wang et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Wang, Sheng-Hann Lee, Chia-Wei Chiou, Arthur Wei, Pei-Kuen Size-dependent endocytosis of gold nanoparticles studied by three-dimensional mapping of plasmonic scattering images |
title | Size-dependent endocytosis of gold nanoparticles studied by three-dimensional mapping of plasmonic scattering images |
title_full | Size-dependent endocytosis of gold nanoparticles studied by three-dimensional mapping of plasmonic scattering images |
title_fullStr | Size-dependent endocytosis of gold nanoparticles studied by three-dimensional mapping of plasmonic scattering images |
title_full_unstemmed | Size-dependent endocytosis of gold nanoparticles studied by three-dimensional mapping of plasmonic scattering images |
title_short | Size-dependent endocytosis of gold nanoparticles studied by three-dimensional mapping of plasmonic scattering images |
title_sort | size-dependent endocytosis of gold nanoparticles studied by three-dimensional mapping of plasmonic scattering images |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236302/ https://www.ncbi.nlm.nih.gov/pubmed/21167077 http://dx.doi.org/10.1186/1477-3155-8-33 |
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