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A kinetic model for quantitative evaluation of the effect of hydrogen and osmolarity on hydrogen production by Caldicellulosiruptor saccharolyticus

BACKGROUND: Caldicellulosiruptor saccharolyticus has attracted increased interest as an industrial hydrogen (H(2)) producer. The aim of the present study was to develop a kinetic growth model for this extreme thermophile. The model is based on Monod kinetics supplemented with the inhibitory effects...

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Autores principales: Ljunggren, Mattias, Willquist, Karin, Zacchi, Guido, van Niel, Ed WJ
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236304/
https://www.ncbi.nlm.nih.gov/pubmed/21914204
http://dx.doi.org/10.1186/1754-6834-4-31
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author Ljunggren, Mattias
Willquist, Karin
Zacchi, Guido
van Niel, Ed WJ
author_facet Ljunggren, Mattias
Willquist, Karin
Zacchi, Guido
van Niel, Ed WJ
author_sort Ljunggren, Mattias
collection PubMed
description BACKGROUND: Caldicellulosiruptor saccharolyticus has attracted increased interest as an industrial hydrogen (H(2)) producer. The aim of the present study was to develop a kinetic growth model for this extreme thermophile. The model is based on Monod kinetics supplemented with the inhibitory effects of H(2 )and osmotic pressure, as well as the liquid-to-gas mass transfer of H(2). RESULTS: Mathematical expressions were developed to enable the simulation of microbial growth, substrate consumption and product formation. The model parameters were determined by fitting them to experimental data. The derived model corresponded well with experimental data from batch fermentations in which the stripping rates and substrate concentrations were varied. The model was used to simulate the inhibition of growth by H(2 )and solute concentrations, giving a critical dissolved H(2 )concentration of 2.2 mmol/L and an osmolarity of 0.27 to 29 mol/L. The inhibition by H(2), being a function of the dissolved H(2 )concentration, was demonstrated to be mainly dependent on H(2 )productivity and mass transfer rate. The latter can be improved by increasing the stripping rate, thereby allowing higher H(2 )productivity. The experimentally determined degree of oversaturation of dissolved H(2 )was 12 to 34 times the equilibrium concentration and was comparable to the values given by the model. CONCLUSIONS: The derived model is the first mechanistically based model for fermentative H(2 )production and provides useful information to improve the understanding of the growth behavior of C. saccharolyticus. The model can be used to determine optimal operating conditions for H(2 )production regarding the substrate concentration and the stripping rate.
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spelling pubmed-32363042011-12-14 A kinetic model for quantitative evaluation of the effect of hydrogen and osmolarity on hydrogen production by Caldicellulosiruptor saccharolyticus Ljunggren, Mattias Willquist, Karin Zacchi, Guido van Niel, Ed WJ Biotechnol Biofuels Research BACKGROUND: Caldicellulosiruptor saccharolyticus has attracted increased interest as an industrial hydrogen (H(2)) producer. The aim of the present study was to develop a kinetic growth model for this extreme thermophile. The model is based on Monod kinetics supplemented with the inhibitory effects of H(2 )and osmotic pressure, as well as the liquid-to-gas mass transfer of H(2). RESULTS: Mathematical expressions were developed to enable the simulation of microbial growth, substrate consumption and product formation. The model parameters were determined by fitting them to experimental data. The derived model corresponded well with experimental data from batch fermentations in which the stripping rates and substrate concentrations were varied. The model was used to simulate the inhibition of growth by H(2 )and solute concentrations, giving a critical dissolved H(2 )concentration of 2.2 mmol/L and an osmolarity of 0.27 to 29 mol/L. The inhibition by H(2), being a function of the dissolved H(2 )concentration, was demonstrated to be mainly dependent on H(2 )productivity and mass transfer rate. The latter can be improved by increasing the stripping rate, thereby allowing higher H(2 )productivity. The experimentally determined degree of oversaturation of dissolved H(2 )was 12 to 34 times the equilibrium concentration and was comparable to the values given by the model. CONCLUSIONS: The derived model is the first mechanistically based model for fermentative H(2 )production and provides useful information to improve the understanding of the growth behavior of C. saccharolyticus. The model can be used to determine optimal operating conditions for H(2 )production regarding the substrate concentration and the stripping rate. BioMed Central 2011-09-13 /pmc/articles/PMC3236304/ /pubmed/21914204 http://dx.doi.org/10.1186/1754-6834-4-31 Text en Copyright ©2011 Ljunggren et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Ljunggren, Mattias
Willquist, Karin
Zacchi, Guido
van Niel, Ed WJ
A kinetic model for quantitative evaluation of the effect of hydrogen and osmolarity on hydrogen production by Caldicellulosiruptor saccharolyticus
title A kinetic model for quantitative evaluation of the effect of hydrogen and osmolarity on hydrogen production by Caldicellulosiruptor saccharolyticus
title_full A kinetic model for quantitative evaluation of the effect of hydrogen and osmolarity on hydrogen production by Caldicellulosiruptor saccharolyticus
title_fullStr A kinetic model for quantitative evaluation of the effect of hydrogen and osmolarity on hydrogen production by Caldicellulosiruptor saccharolyticus
title_full_unstemmed A kinetic model for quantitative evaluation of the effect of hydrogen and osmolarity on hydrogen production by Caldicellulosiruptor saccharolyticus
title_short A kinetic model for quantitative evaluation of the effect of hydrogen and osmolarity on hydrogen production by Caldicellulosiruptor saccharolyticus
title_sort kinetic model for quantitative evaluation of the effect of hydrogen and osmolarity on hydrogen production by caldicellulosiruptor saccharolyticus
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236304/
https://www.ncbi.nlm.nih.gov/pubmed/21914204
http://dx.doi.org/10.1186/1754-6834-4-31
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