Cargando…

IGF-1 Induction by Acylated Steryl β-Glucosides Found in a Pre-Germinated Brown Rice Diet Reduces Oxidative Stress in Streptozotocin-Induced Diabetes

BACKGROUND: The pathology of diabetic neuropathy involves oxidative stress on pancreatic β-cells, and is related to decreased levels of Insulin-like growth factor 1 (IGF-1). Acylated steryl β-glucoside (PR-ASG) found in pre-germiated brown rice is a bioactive substance exhibiting properties that enh...

Descripción completa

Detalles Bibliográficos
Autores principales: Usuki, Seigo, Tsai, Ying-Ying, Morikawa, Keiko, Nonaka, Shota, Okuhara, Yasuhide, Kise, Mitsuo, Yu, Robert K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3237479/
https://www.ncbi.nlm.nih.gov/pubmed/22194889
http://dx.doi.org/10.1371/journal.pone.0028693
_version_ 1782218903007526912
author Usuki, Seigo
Tsai, Ying-Ying
Morikawa, Keiko
Nonaka, Shota
Okuhara, Yasuhide
Kise, Mitsuo
Yu, Robert K.
author_facet Usuki, Seigo
Tsai, Ying-Ying
Morikawa, Keiko
Nonaka, Shota
Okuhara, Yasuhide
Kise, Mitsuo
Yu, Robert K.
author_sort Usuki, Seigo
collection PubMed
description BACKGROUND: The pathology of diabetic neuropathy involves oxidative stress on pancreatic β-cells, and is related to decreased levels of Insulin-like growth factor 1 (IGF-1). Acylated steryl β-glucoside (PR-ASG) found in pre-germiated brown rice is a bioactive substance exhibiting properties that enhance activity of homocysteine-thiolactonase (HTase), reducing oxidative stress in diabetic neuropathy. The biological importance of PR-ASG in pancreatic β-cells remains unknown. Here we examined the effects of PR-ASG on IGF-1 and glucose metabolism in β-cells exposed to oxidative stress. METHODOLOGY/PRINCIPAL FINDINGS: In the present study, a pre-germinated brown rice (PR)-diet was tested in streptozotocin (STZ)-induced diabetic rats. Compared with diabetic rats fed control diets, the PR-diet fed rats showed an improvement of serum metabolic and neurophysiological parameters. In addition, IGF-1 levels were found to be increased in the serum, liver, and pancreas of diabetic rats fed the PR-diet. The increased IGF-1 level in the pancreas led us to hypothesize that PR-ASG is protective for islet β-cells against the extensive injury of advanced or severe diabetes. Thus we examined PR-ASG to determine whether it showed anti-apoptotic, pro-proliferative effects on the insulin-secreting β-cells line, INS-1; and additionally, whether PR-ASG stimulated IGF-1 autocrine secretion/IGF-1-dependent glucose metabolism. We have demonstrated for the first time that PR-ASG increases IGF-1 production and secretion from pancreatic β-cells. CONCLUSION/SIGNIFICANCE: These findings suggest that PR-ASG may affect pancreatic β-cells through the activation of an IGF-1-dependent mechanism in the diabetic condition. Thus, intake of pre-germinated brown rice may have a beneficial effect in the treatment of diabetes, in particular diabetic neuropathy.
format Online
Article
Text
id pubmed-3237479
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-32374792011-12-22 IGF-1 Induction by Acylated Steryl β-Glucosides Found in a Pre-Germinated Brown Rice Diet Reduces Oxidative Stress in Streptozotocin-Induced Diabetes Usuki, Seigo Tsai, Ying-Ying Morikawa, Keiko Nonaka, Shota Okuhara, Yasuhide Kise, Mitsuo Yu, Robert K. PLoS One Research Article BACKGROUND: The pathology of diabetic neuropathy involves oxidative stress on pancreatic β-cells, and is related to decreased levels of Insulin-like growth factor 1 (IGF-1). Acylated steryl β-glucoside (PR-ASG) found in pre-germiated brown rice is a bioactive substance exhibiting properties that enhance activity of homocysteine-thiolactonase (HTase), reducing oxidative stress in diabetic neuropathy. The biological importance of PR-ASG in pancreatic β-cells remains unknown. Here we examined the effects of PR-ASG on IGF-1 and glucose metabolism in β-cells exposed to oxidative stress. METHODOLOGY/PRINCIPAL FINDINGS: In the present study, a pre-germinated brown rice (PR)-diet was tested in streptozotocin (STZ)-induced diabetic rats. Compared with diabetic rats fed control diets, the PR-diet fed rats showed an improvement of serum metabolic and neurophysiological parameters. In addition, IGF-1 levels were found to be increased in the serum, liver, and pancreas of diabetic rats fed the PR-diet. The increased IGF-1 level in the pancreas led us to hypothesize that PR-ASG is protective for islet β-cells against the extensive injury of advanced or severe diabetes. Thus we examined PR-ASG to determine whether it showed anti-apoptotic, pro-proliferative effects on the insulin-secreting β-cells line, INS-1; and additionally, whether PR-ASG stimulated IGF-1 autocrine secretion/IGF-1-dependent glucose metabolism. We have demonstrated for the first time that PR-ASG increases IGF-1 production and secretion from pancreatic β-cells. CONCLUSION/SIGNIFICANCE: These findings suggest that PR-ASG may affect pancreatic β-cells through the activation of an IGF-1-dependent mechanism in the diabetic condition. Thus, intake of pre-germinated brown rice may have a beneficial effect in the treatment of diabetes, in particular diabetic neuropathy. Public Library of Science 2011-12-14 /pmc/articles/PMC3237479/ /pubmed/22194889 http://dx.doi.org/10.1371/journal.pone.0028693 Text en Usuki et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Usuki, Seigo
Tsai, Ying-Ying
Morikawa, Keiko
Nonaka, Shota
Okuhara, Yasuhide
Kise, Mitsuo
Yu, Robert K.
IGF-1 Induction by Acylated Steryl β-Glucosides Found in a Pre-Germinated Brown Rice Diet Reduces Oxidative Stress in Streptozotocin-Induced Diabetes
title IGF-1 Induction by Acylated Steryl β-Glucosides Found in a Pre-Germinated Brown Rice Diet Reduces Oxidative Stress in Streptozotocin-Induced Diabetes
title_full IGF-1 Induction by Acylated Steryl β-Glucosides Found in a Pre-Germinated Brown Rice Diet Reduces Oxidative Stress in Streptozotocin-Induced Diabetes
title_fullStr IGF-1 Induction by Acylated Steryl β-Glucosides Found in a Pre-Germinated Brown Rice Diet Reduces Oxidative Stress in Streptozotocin-Induced Diabetes
title_full_unstemmed IGF-1 Induction by Acylated Steryl β-Glucosides Found in a Pre-Germinated Brown Rice Diet Reduces Oxidative Stress in Streptozotocin-Induced Diabetes
title_short IGF-1 Induction by Acylated Steryl β-Glucosides Found in a Pre-Germinated Brown Rice Diet Reduces Oxidative Stress in Streptozotocin-Induced Diabetes
title_sort igf-1 induction by acylated steryl β-glucosides found in a pre-germinated brown rice diet reduces oxidative stress in streptozotocin-induced diabetes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3237479/
https://www.ncbi.nlm.nih.gov/pubmed/22194889
http://dx.doi.org/10.1371/journal.pone.0028693
work_keys_str_mv AT usukiseigo igf1inductionbyacylatedsterylbglucosidesfoundinapregerminatedbrownricedietreducesoxidativestressinstreptozotocininduceddiabetes
AT tsaiyingying igf1inductionbyacylatedsterylbglucosidesfoundinapregerminatedbrownricedietreducesoxidativestressinstreptozotocininduceddiabetes
AT morikawakeiko igf1inductionbyacylatedsterylbglucosidesfoundinapregerminatedbrownricedietreducesoxidativestressinstreptozotocininduceddiabetes
AT nonakashota igf1inductionbyacylatedsterylbglucosidesfoundinapregerminatedbrownricedietreducesoxidativestressinstreptozotocininduceddiabetes
AT okuharayasuhide igf1inductionbyacylatedsterylbglucosidesfoundinapregerminatedbrownricedietreducesoxidativestressinstreptozotocininduceddiabetes
AT kisemitsuo igf1inductionbyacylatedsterylbglucosidesfoundinapregerminatedbrownricedietreducesoxidativestressinstreptozotocininduceddiabetes
AT yurobertk igf1inductionbyacylatedsterylbglucosidesfoundinapregerminatedbrownricedietreducesoxidativestressinstreptozotocininduceddiabetes