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Selection-Independent Generation of Gene Knockout Mouse Embryonic Stem Cells Using Zinc-Finger Nucleases

Gene knockout in murine embryonic stem cells (ESCs) has been an invaluable tool to study gene function in vitro or to generate animal models with altered phenotypes. Gene targeting using standard techniques, however, is rather inefficient and typically does not exceed frequencies of 10(−6). In conse...

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Detalles Bibliográficos
Autores principales:	Osiak, Anna, Radecke, Frank, Guhl, Eva, Radecke, Sarah, Dannemann, Nadine, Lütge, Fabienne, Glage, Silke, Rudolph, Cornelia, Cantz, Tobias, Schwarz, Klaus, Heilbronn, Regine, Cathomen, Toni
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3237556/ https://www.ncbi.nlm.nih.gov/pubmed/22194948 http://dx.doi.org/10.1371/journal.pone.0028911

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