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Quantification Bias Caused by Plasmid DNA Conformation in Quantitative Real-Time PCR Assay

Quantitative real-time PCR (qPCR) is the gold standard for the quantification of specific nucleic acid sequences. However, a serious concern has been revealed in a recent report: supercoiled plasmid standards cause significant over-estimation in qPCR quantification. In this study, we investigated th...

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Detalles Bibliográficos
Autores principales:	Lin, Chih-Hui, Chen, Yu-Chieh, Pan, Tzu-Ming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3237602/ https://www.ncbi.nlm.nih.gov/pubmed/22194997 http://dx.doi.org/10.1371/journal.pone.0029101

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